RPOC2_CERDE
ID RPOC2_CERDE Reviewed; 1390 AA.
AC A8SE78;
DT 04-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT 15-JAN-2008, sequence version 1.
DT 03-AUG-2022, entry version 51.
DE RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324};
OS Ceratophyllum demersum (Rigid hornwort) (Coontail).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Ceratophyllales; Ceratophyllaceae;
OC Ceratophyllum.
OX NCBI_TaxID=4428;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=18048334; DOI=10.1073/pnas.0708072104;
RA Moore M.J., Bell C.D., Soltis P.S., Soltis D.E.;
RT "Using plastid genome-scale data to resolve enigmatic relationships among
RT basal angiosperms.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:19363-19368(2007).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC Rule:MF_01324}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR EMBL; EF614270; ABQ81440.1; -; Genomic_DNA.
DR RefSeq; YP_001542437.1; NC_009962.1.
DR AlphaFoldDB; A8SE78; -.
DR GeneID; 5729432; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 1.
DR TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Metal-binding;
KW Nucleotidyltransferase; Plastid; Transcription; Transferase; Zinc.
FT CHAIN 1..1390
FT /note="DNA-directed RNA polymerase subunit beta''"
FT /id="PRO_0000353551"
FT BINDING 224
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 294
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 301
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 304
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ SEQUENCE 1390 AA; 157648 MW; 93BC82F96199A807 CRC64;
MEVLMAERAD LVFHNKAIDG TAMKRLISRL IDHFGMAYTS HILDQVKTLG FQQATATSIS
LGIDDLLTIP SKGWLVQDAE QQSLTLEKNH HYGNVHAVEK LRQSIEIWYA TSEYLRQEMN
PNFRMTDPSN PVHIMSFSGA RGNASQVHQL VGMRGLMSDP QGQMIDLPIQ SNLREGLSLT
EYIISCYGAR KGVVDTAVRT SDAGYLTRRL VEVVQHIVVR RTDCGTIRRI SVSPQNGMTE
KIFIQTLIGR VLADDIYIGP RCIATRNQDI GIGLINRFIT FRAQPIYIRT PFTCRSTSWI
CRLCYGRSPT HGDLVELGEA VGIIAGQSIG EPGTQLTLRT FHTGGVFTGG TAEHVRAPSN
GKIKFNEDLV HPTRTRHGHP AFLCYIDLYV TIESQSIIHN VNIPPKSLIL VQNDQYVESE
QVIAEIRTGA STFHFKERVR KHIYSDSQGE MHWSTDVYHA PEYTYGNVHL LPKTSHLWIL
SGGPCRSSIV PFSIQKDQDQ INVNSLFVEQ RYISDLSVTN DRVRHKIFSS DPSGQKGGRI
FDYDSGPDRI VSNSQFLYPA IPHENSDLLA KRRRNRFLIP FQYDQEQEKE LMCPYGISTE
IPINGILRRN SILAYFDDPR YRRSSSGITK YATIEVDSIV KKEDLIEYRG SKEFRPKYQT
KVDRFFFIPE EVHILPGSSS IMVRNNSIIG VDTRITLNIR SRVGGLVRVE RKKKRIELKI
FSGDIHFPGE TDKISRHSGI LIPPGVVKKN SKKSKKKWKN WIYIQRITPT KKKYFVSVRP
VVTYEIADGI NLPTLFPQDL LQERDNMQLQ VVNYIISGNG KPSRGIPQTS IQLVRTCLVL
NWDQDLKGSL EEIHASFVEV RTNDLIRDFI RIDLVKSPIS YTGKRNDTWG SGLNPDSISD
CTNINPFYSK ARLPSFTQPK GTVRTLRNKE YQSLIILSSS DCYQIGPFKD SKYNKTTNCH
NITKELIKED SPVPISNSLG PLGAVPKFLN FDSFSHLITH NQILLKKYLL LDNLKHTSQV
MKYFLLDENG KIHNTDPCRN IFFNPSHLNW YSLHHDFFYE TPITISLGQF ICENVYIFEY
GPHIKSGQVL IVQFDSLVIR SAKPHLATPG ATVHGHYGEI LYEGDTLVTF IYEKSRSGDI
TQGLPKVEQV LEVRSIDSIS LNLEKRVEGW NERVTRILGI PWGFLIGAEL TIAQSCISLV
NKIQKVYRSQ GVQIHNRHIE IVVRQITSKV LVSEDGMSNV FSPGELIGLL RAERTGRALE
EAIRYRAILV GITRASLNTQ SFISEASFQE TARVLAKAAL RGRIDWLKGL KENVVLGGMI
PVGTGFKGLV HHSRQHNNIP LEIKKRNLFE FEGEMGDILF HHRQSFGSCI QKTFHETSEQ
SFGFGRFNDS
