RPOC2_CHAGL
ID RPOC2_CHAGL Reviewed; 1373 AA.
AC Q8MA10;
DT 16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 70.
DE RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324};
OS Chaetosphaeridium globosum (Charophycean green alga) (Herposteiron
OS globosum).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Coleochaetophyceae; Coleochaetales;
OC Chaetosphaeridiaceae; Chaetosphaeridium.
OX NCBI_TaxID=96477;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=M1311;
RX PubMed=12161560; DOI=10.1073/pnas.162203299;
RA Turmel M., Otis C., Lemieux C.;
RT "The chloroplast and mitochondrial genome sequences of the charophyte
RT Chaetosphaeridium globosum: insights into the timing of the events that
RT restructured organelle DNAs within the green algal lineage that led to land
RT plants.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:11275-11280(2002).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC Rule:MF_01324}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR EMBL; AF494278; AAM96568.1; -; Genomic_DNA.
DR RefSeq; NP_683776.1; NC_004115.1.
DR AlphaFoldDB; Q8MA10; -.
DR PRIDE; Q8MA10; -.
DR GeneID; 860724; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 1.
DR TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Metal-binding;
KW Nucleotidyltransferase; Plastid; Transcription; Transferase; Zinc.
FT CHAIN 1..1373
FT /note="DNA-directed RNA polymerase subunit beta''"
FT /id="PRO_0000067918"
FT BINDING 220
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 293
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 300
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 303
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ SEQUENCE 1373 AA; 158518 MW; 3A6E0B7FA534F961 CRC64;
MSKKTKILYY NQVMDRSGMK NLISRLISYI GVTSASEVLD QLKTIGFNYA TKFGISLGID
DLLTTPFKGW FMQDAEKHAS ISEKHNRYGT IHAVEKLRQL IETWYTTSEY LKQEMSPNFK
ITDPYNPVHM MSFSGARGNA SQVHQLVGMR GLMADPQGQI IDLPIQSNFR EGLSLTEYII
SCYGARKGVV DTAVRTADAG YLTRRLVEVV QHIVVRKIDC GSIDGIQLKP LKEKKIKNLF
GSADRLVGRV LATDLFIDKR CIAKRNQDIG SELAINIINY QKKAILIRSP LTCKSKQWLC
QYCYGWSPCY GDMVELGEAV GIIAGQSIGE PGTQLTLRTF HTGGVFTGDI AEHVRAPFNG
IAFFSETNLQ ATRSRHGHPA WISKEDIKIT IQYEEIEHKL NIPANSLILI KTNQYVKSKQ
VLAEVRAKIA PLKEQIEKYI YSDLSGEIHW GFPVRHTSEY IYSNLHTVQN TGHIWVVSGN
IKNSNKNLLL FYKKGDYILK DFLNKKLNIN FQVYNKKLNF YQKSIRNSKK NINNLINSTE
FLNINSSAIF ATYRYKNTKM NENNPNIFSE TNLNKYSLKK FYNFVLEKNQ KFISNNTKIA
SSSIYKHHIE NCGIIKYGSI QIKPFPSNFD QDLNTIKEKI KPLKLKYELI NSGTIFWIPE
YVHTLVCSRS SILVQDGMFI EAGTEIFRGF FMPESGLVQI KSKTNKIHEI TIVPGKLLDS
IDIFDNLKKQ KFFFYPGEEI IPGIKAEVLT YLYLFGVNKQ KNKLLLRPAK EFFIEKLRKN
PINLIPIFGT KKDIKIALFE FIVYEDGQQV RSKGKIELVQ TCLITNIKKG PYSENAKFFS
IDVFFKNLRN RFLNFCFSNQ TNFEKMLSKR NFHKNLYFCF KTLNNKNKEN LANKKNKDSL
IQNKGYLQLL ETKNTINKNL LFVSRKNQIQ ILYNKITKED LKENYKDINT ISKIKKTKIY
HTDQQVFTIQ SFYLLNKNLG LLGNFYKNKL LFQFIFDYNK IKTYKKDLSL DINTNLLKNK
NWFLIDESNN FFQLKTRKNQ NVKQKKIEKD IRINLGNFFS QNVVLFNTRQ LPESGQILYI
EKKICVFRLC RPYLATKNAI IYGNYKQIIT SGDNFITIVY ERFKTRDIIQ GLPKVEQLLE
ARPLNNLSSN LDFGFLDWNT DISRLLGNPW GFFLSSLIII RQSQLELVNQ IQKVYQSQGV
QISDKHLEII VRQMTSRVLT TEENGPNAFL PGELVDLFKA QRMNQILENS LAYKPILLGI
TRASLNTYSF ISEASFQETT KVLTKAALQG RIDWLQGLKE NVILGGIIPA GTGYQKILNK
KNSNFLENKK EVSIFVQKQK NIKFNILPSN TEVNKKESIQ NLLKKLHITY TFQ
