RPOC2_CHLSC
ID RPOC2_CHLSC Reviewed; 1389 AA.
AC A6MMB2;
DT 04-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-JUL-2007, sequence version 1.
DT 03-AUG-2022, entry version 48.
DE RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324};
OS Chloranthus spicatus (Chulantree) (Nigrina spicata).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Chloranthales; Chloranthaceae; Chloranthus.
OX NCBI_TaxID=13006;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=17644003; DOI=10.1016/j.ympev.2007.06.004;
RA Hansen D.R., Dastidar S.G., Cai Z., Penaflor C., Kuehl J.V., Boore J.L.,
RA Jansen R.K.;
RT "Phylogenetic and evolutionary implications of complete chloroplast genome
RT sequences of four early-diverging angiosperms: Buxus (Buxaceae),
RT Chloranthus (Chloranthaceae), Dioscorea (Dioscoreaceae), and Illicium
RT (Schisandraceae).";
RL Mol. Phylogenet. Evol. 45:547-563(2007).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC Rule:MF_01324}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR EMBL; EF380352; ABQ43250.1; -; Genomic_DNA.
DR RefSeq; YP_001294088.1; NC_009598.1.
DR AlphaFoldDB; A6MMB2; -.
DR GeneID; 5236457; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Metal-binding;
KW Nucleotidyltransferase; Plastid; Transcription; Transferase; Zinc.
FT CHAIN 1..1389
FT /note="DNA-directed RNA polymerase subunit beta''"
FT /id="PRO_0000353552"
FT BINDING 220
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 290
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 297
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 300
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ SEQUENCE 1389 AA; 157297 MW; B9428D49C3AC313A CRC64;
MAERADLVFH NKVIDGTAMK RLISRLIDHF GMAYTSHILD QVKTLGFQQA TATSISLGID
DLLTIPSKGW LVQDAEQQSL ILEKYHHYGN VHAVEKLRQS IEIWYATSEY LRQEMNPNFR
MTDPSNPVHI MSFSGARGNA SQVHQLVGMR GLMSDPQGQM IDLPIQSNLR EGLSLTEYII
SCYGARKGVV DTAVRTSDAG YLTRRLVEVV QHIVVRRTDC GTIRGIFVSP QNGMAKRIFI
QTLIGRVLAD DIYMGPRCIA ARNQYIGIGL VNRFITFRAQ PISIRTPFTC RSTSWICRLC
YGRSPTHGDL VELGEAVGII AGQSIGEPGT QLTLRTFHTG GVFTGGTAEH VRAPSNGKIK
FNEDLVHPTR TRHGHPAFLC YIDLYVTIEN QDIIHNVNIP PKSFLLVQND QYVESEQVIA
EIRAGASTLN FKERVRKHIY SDSEGEMHWS TDVYHASEYT YGNVHLLPKT SHLWILSGSP
YRSSIVPFSL HKDQDQMNVH SLSVERGSIS DLSVTNDRVR HKLFSSDLSG KKRGGILDYS
GPDRIISNGH GHWNFIYPAI LHENSDLLAK RRRNRFIIPF QYDQEREKEL MPRSGISIEI
PINGIFRRKS ILAYFDDPRY RKSSSGITKY GTIEVDSIVK KEDLIEYRGA KEFRPKYQMK
VDRFFFIPEE VHILTGSSSI MVRNNSIIGV DTRIALNTRS RVGGLVRVEK KKKRIELKIF
SGDIHFPGET DKISRHSGIL IPPGTGKKNP KESKKWKNWI YVQQITPTKK KYFVSVRSVV
TYEIADGINL ATLFPQDLLQ ERDNVQLRVV NYILYGNGKP IRGISHTSIQ LVRTCLVLNW
DQDRNGSIEE AHASFVEVRA NALIRDFIRI HLVKSPISYT VKRNDTASSG LIPDNASDRP
NINPFDFKAR VQSLTQHQGT IRTLLNRNKE CQSLIILSSS NCSRLGPFNG SKYHNVTKES
IQTKDKEDPS ISIRNSLGPL GIVPKIANFY YLMTHNQILL NKYLLLDNLK QTFQVLQYYQ
VIKYSLMDEN GQIYNPDPYS NTILNPFDLN WRFLHHDYCE ETSTIISLGQ FICENGCISK
YGPHIESGQV LIVHVDSLVI RSAKPHLATP GATVHGHYGE ILYEGDTLVT FIYEKSRSGD
ITQGLPKVEQ VLEVRSIDSI SINLEKRVEG WNERITRIIG IPWGFLIGAE LTIAQSRISL
VNKIQKVYRS QGVQIHNRHI EIIVRQITSK VLVSEDGMSN VFSPGELIGL LRAERTGRAL
EEAICYRAIL LGITRTSLNT QSFISEASFQ ETARVLAKAA LRGRIDWLKG LKENVVLGGM
IPVGTGFKGL VHRSRQHNNS PLEIKKKNLF EGEMRDILFH HRELFGSCIP NHFHDTSEQS
FTFTGFHDS
