RPOC2_CITSI
ID RPOC2_CITSI Reviewed; 1403 AA.
AC Q09MI8;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 17-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 53.
DE RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324};
OS Citrus sinensis (Sweet orange) (Citrus aurantium var. sinensis).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Sapindales; Rutaceae; Aurantioideae; Citrus.
OX NCBI_TaxID=2711;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Osbeck var. Ridge Pineapple;
RX PubMed=17010212; DOI=10.1186/1471-2229-6-21;
RA Bausher M.G., Singh N.D., Lee S.-B., Jansen R.K., Daniell H.;
RT "The complete chloroplast genome sequence of Citrus sinensis (L.) Osbeck
RT var 'Ridge Pineapple': organization and phylogenetic relationships to other
RT angiosperms.";
RL BMC Plant Biol. 6:21-21(2006).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC Rule:MF_01324}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR EMBL; DQ864733; ABI49010.1; -; Genomic_DNA.
DR RefSeq; YP_740465.1; NC_008334.1.
DR AlphaFoldDB; Q09MI8; -.
DR GeneID; 4271233; -.
DR KEGG; cit:4271233; -.
DR OrthoDB; 731145at2759; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Metal-binding;
KW Nucleotidyltransferase; Plastid; Transcription; Transferase; Zinc.
FT CHAIN 1..1403
FT /note="DNA-directed RNA polymerase subunit beta''"
FT /id="PRO_0000277186"
FT BINDING 220
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 293
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 300
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 303
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ SEQUENCE 1403 AA; 158670 MW; 277E48C9DC6FB3A3 CRC64;
MAERVSLVFH NKMIDGTAIK RLISRLIDHF GMAYTSHILD QVKTLGFQQA TATSISLGID
DLLTIPSKRW LVQDAEQQSF ILEKHYHYGN VHAIEKLRQS IEIWYATSEY LRQEMNPNFR
MTEPFNPVHI MSFSGARGNA SQVHQLVGMR GLMSDPQGQM IDLPIQSNLR EGLSLTEYII
SCYGARKGVV DTAVRTSDAG YLTRRLVEVV QHIIVRRTDC GTVRGISVSP QNQNRMMSER
VFSQTLIGRV LADDIYMGQR SIAIRNQDIG IGLVNRLITL RTQAISIRTP FTCRSTSWIC
RLCYGRSPTH GDLVELGEAV GIIAGQSIGE PGTQLTLRTF HTGGVFTGGT AEHVRAPSNG
KIKFNEDLVH PTRTRHGHPA FLCSIDLDVI IEGEDIMHNV TIPPKSFLLV QNDQYVESEQ
VIAEIRAGAY TLNFKERVRK HIYSDSEGEM HWSTDVYHAP EFTYSNVHLL PKTSHLWILS
GGSRRSSVVS FSLHKDQDQM NIHSLSVERR EISSLSLSVN NDQTGHKFFS SDFSAKKKGR
ILEMSDYSGF NRIISTGHCN LIHPAILHAN SDLLAKRQRN GFLIPFHSIQ EQEKELMPHS
GISIEIPVRG IFRRNSILAY FDDPRYRRKS SGITKWGTLG AHSIVKKEDL IEYRGLKKIK
PKYQMKIDRL FFIPEEVHIF PESSYLMVRN NSIIGVDTQI TLNIRSRVGG LVRMERKRGG
VELKIFSGDI HFPGEIDKIS RHSGILIPPE SEKTKLKEAT KESKKLKKWI YVQRITPTKK
KSFVLVRPVV TYEIANGINL ATLFPQDPLR EKDNMQFRVV NYVLYGKGKA AGGISDTSLQ
LVRTCLVLNW DQDNKSSSVE EVCASFVEVR TNGLIRNFLR INLVKSNISY LRKRDHPSGS
GLISDNGSAR TNSNPFYSVF GKAGVEQSLR QNQGTIRTLL NKNKECQVLI ILSSSNYFRM
GPLNDVKYHN VIKQSIPIQK DSLTPIKTSL GPLGTFLQIA NFYSFYYLIT HNHISVTKYL
KLDNLKQPFQ VLKYYLMDET GEIYNPDTDS KILLNPFNLN WYFLQPNYCE EMSPIISLGQ
FLCENVRITK RGPYLKSGQV LIVQVNSVVI RSAKPYLATP GATVHGHYGA ILYEGDTLVT
FIYEKSRSGD ITQGLPKVEQ VLEVRSLDSI SMNLEKRVEG WNASITRILG IPWGFLIGAE
LTIVQSRISL VNKIQKVYRS QGVQIHNRHI EIIVRQITSK VLVSEDGMSN IFLPGELIGL
LRAERMGRAL EEVICYRAIL LGITRASLNT QSFISEASFQ ETTRVLAKAA LRGRIDWLKG
LKENVVLGGI IPVGTGFKGL VHCSRQHNNI LLERQKGNLF GGEMRDIFLH HRELFDSCIS
TTFHDTSEHN CLEGLMSPRS EFF