ID   RPOC2_CUSRE             Reviewed;        1379 AA.
AC   A7M956;
DT   04-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT   02-OCT-2007, sequence version 1.
DT   03-AUG-2022, entry version 48.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE   AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE            Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN   Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324};
OS   Cuscuta reflexa (Southern Asian dodder).
OG   Plastid.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Convolvulaceae; Cuscuteae; Cuscuta;
OC   Cuscuta subgen. Monogynella.
OX   NCBI_TaxID=4129;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=17714582; DOI=10.1186/1471-2229-7-45;
RA   Funk H.T., Berg S., Krupinska K., Maier U.-G., Krause K.;
RT   "Complete DNA sequences of the plastid genomes of two parasitic flowering
RT   plant species, Cuscuta reflexa and Cuscuta gronovii.";
RL   BMC Plant Biol. 7:45-45(2007).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01324}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC       Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01324}.
CC   -!- SUBCELLULAR LOCATION: Plastid.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
CC   -!- CAUTION: Young tissue from this organism is photosynthetic and contains
CC       some thylakoids, although the photosynthetic activity does not exceed
CC       the light compensation point. {ECO:0000305}.
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DR   EMBL; AM711640; CAM98384.1; -; Genomic_DNA.
DR   RefSeq; YP_001430098.1; NC_009766.1.
DR   AlphaFoldDB; A7M956; -.
DR   PRIDE; A7M956; -.
DR   GeneID; 5536697; -.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0009536; C:plastid; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 1.10.132.30; -; 1.
DR   Gene3D; 1.10.274.100; -; 1.
DR   HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR   InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR   InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR   InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR   InterPro; IPR007083; RNA_pol_Rpb1_4.
DR   InterPro; IPR007081; RNA_pol_Rpb1_5.
DR   InterPro; IPR038120; Rpb1_funnel_sf.
DR   PANTHER; PTHR19376; PTHR19376; 1.
DR   Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR   Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR   TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Metal-binding; Nucleotidyltransferase;
KW   Plastid; Transcription; Transferase; Zinc.
FT   CHAIN           1..1379
FT                   /note="DNA-directed RNA polymerase subunit beta''"
FT                   /id="PRO_0000353557"
FT   BINDING         220
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT   BINDING         291
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT   BINDING         298
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT   BINDING         301
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ   SEQUENCE   1379 AA;  155934 MW;  A0BF91E6C05CCB89 CRC64;
     MAERINLVFH NKVLDGTAMK RLISRLIDHF GMAYTSHILD QVKTLGFQQA TATSISLGID
     DLLTIPSKGW LVQDAEQQSF VFEKYQHSGN VHAVEKLRQS IEIWYATSEY LRQEMHLNFR
     MTDPLNPVHI MSFSGARGNA SQVHQLVGMR GLMSDPQGQM IDLPIQSNLR EGLSLTEYII
     SCYGARKGVV DTAVRTSDAG YLTRRLVEVV QHIVVRRADC GTARGISVSP HNGMMPERIF
     IQTLIGRVLA DDIYIGPRCI ATRNQNIGVG LVNKLLNFRA QPISIRTPFT CRSTSWICRL
     CYGRSPTHGD LVELGEAVGI IAGQSIGEPG TQLTLRTFHT GGVFTGGTAE HVRAPSNGKI
     RFTEDLVHPT RTRHGYPAFF CSIYLYVTIQ SQDILHHVKI PPKSFILVQN DQYVESEQVI
     AETRAGASTL NYKEKIRKHI YSDSGGEMHW STNVYHAPEF TYGNVHLLSK TSHLWILLGE
     PCHSSLVSTS IHRDQDQMSA QSLSVKRRYT SKLSETNDEA KQKIASEDLI ADYSDVNPSR
     CTDHYNLVYP AILPILDENS YFFSNCLSKR RRNQFIIPLQ SIQEHKNQLM PCSSISMKIP
     PNANGIFCAN SILAYFDDPR YRRNNSGSTK YGTLEMYSTI KKEDFIQYRG VNEFRLKSKV
     ERFFFIPEEV HILPGSSSIM VRNQSLIGVD TQITLNLRSR VGGLVWVERK KNRIELKIFF
     GDIYFPGGAD NLSRHSGVLI PPGTEIKTNY KESKKVKNWI YVQRITLSKK KFFFLVRPLV
     TYEIMDGIPL ATLFPPDLLQ QRENAQLRVV NYILHGNGKP IRGNYDASIQ LVRTCLVFKR
     NQDKKSSYSE AARASVVEIR TNYLIRHFLR IDFVKAPISY IGKRYDPLGL GLLAEDWTHK
     NPYSKARIHQ NLNQNKGTIH TFFNRNKGSQ SLIILSSSNC SRMDPANGAK SNNVIQESKK
     EEYPMLQISN SLGPLGTYPP IANCDSFNRL LTHNQILVTN YFHLDNVKPP FQVFKFKYYF
     IAENLKICNY NPCSNLRLNA FYLNLNFLHP NSCAETSKIM SLGQFICQNV CIDKTRPPFK
     SGQVIFIQVD SVVIRLAKPY LATPGATVHG LYGETFFGGD TVVTFNYEKS RSGDITQGLP
     KVEQVLEVRS VDSISMNLEG RVEGWGKCIT GILGIPWGFL IGAELTIVQS RISLVNKIQK
     VYRFQGVHIH NRHIEIIVRQ ITSKVLVSED GMSNVFLPGE LIGLLRAERM GRALEEPIHY
     RSVFLGITKA SLNTQSFISE ASFQETARVL SKAALGGRID WLKGLKENVV LGGVIPAGTG
     FRGLVDPSKQ YNKNNIPLKN NLFEGGMTDL LVHHRKLFDS FLNTLIYHHH RIDRFYNDS