RPOC2_DAUCA
ID RPOC2_DAUCA Reviewed; 1390 AA.
AC Q0G9X2;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 56.
DE RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324};
OS Daucus carota (Wild carrot).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; campanulids; Apiales; Apiaceae; Apioideae; Scandiceae; Daucinae;
OC Daucus; Daucus sect. Daucus.
OX NCBI_TaxID=4039;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Danvers Half-long;
RX PubMed=16945140; DOI=10.1186/1471-2164-7-222;
RA Ruhlman T., Lee S.-B., Jansen R.K., Hostetler J.B., Tallon L.J., Town C.D.,
RA Daniell H.;
RT "Complete plastid genome sequence of Daucus carota: implications for
RT biotechnology and phylogeny of angiosperms.";
RL BMC Genomics 7:222-222(2006).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC Rule:MF_01324}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR EMBL; DQ898156; ABI32414.1; -; Genomic_DNA.
DR RefSeq; YP_740107.1; NC_008325.1.
DR AlphaFoldDB; Q0G9X2; -.
DR GeneID; 4266717; -.
DR OrthoDB; 731145at2759; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Metal-binding;
KW Nucleotidyltransferase; Plastid; Transcription; Transferase; Zinc.
FT CHAIN 1..1390
FT /note="DNA-directed RNA polymerase subunit beta''"
FT /id="PRO_0000277188"
FT BINDING 224
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 295
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 302
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 305
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ SEQUENCE 1390 AA; 157778 MW; 58E7AC3A23023ADF CRC64;
MEVLMAERAN LVFHNKVIDG TAMKRLISRL IDHFGMAYTS HILDQVKTLG FQQATATSIS
LGIDDLLTIP SKRWLVQDAE EQSFILEKHH HSGNVHAVEK LRQSIEIWYA TSEFLRQEMN
PNFRMTDPFN PVHIMSFSGA RGNASQVHQL VGMRGLMSDP QGQMIDLPIQ SNLREGLSLT
EYIISCYGAR KGVVDTAVRT SDAGYLTRRL VEVVQHIVVR RKDCGTARGI SVSLGNGMMS
ENIFIQTLIG RVLADDIYMG TRCIATRNQD IGSGLVNQFI TFRAQPIYIR TPFTCRSTSW
ICQLCYGRSP THGDLVELGE AVGIIAGQSI GEPGTQLTLR TFHTGGVFTG GIAEHVRAPS
NGKIKFNEDL VHPTRTRHGH PAFVCSIDLY VTIESEDILH NVNIPPKSFL LVQNDQYVES
EQVIAEIRAG TSTLNFKEKV RKHIYSDSEG EMHWNTDVYH APEFTYGNVH LLPKTSHLWI
LLGEPRRSDL ISLSIHKDQD QMNARSFSVK KRSISNLSVT NDQVRHKFFS SDFFGKKEEE
HPDYSELNRI VRCNLRYPTI PYADYDLLAK RRRKRFIIPL QSIQERENEL MPPSGISIEI
PINGIFRINS ILAFFDDPRY RRKSSGITKY GTIEVDPIAK KEDLIEYRGV KEFKPKYQIK
VDRFFFIPEE MHILPGSSSI MVRNNSIIGI DTQIALTTRS RVGGLVRVEI KKKRIELKIF
SGDIHFPGET DKISRHSGVL IPPGTGKTNS KESKKGKNWI YVQRITPTNK KYFVLVRPVV
TYEITDGINL VRLFPPDLLQ EMDNVQLRVV NYILYGNGKP IREIYNTSIQ LVRTCLVLNC
TQDKKSSYIE ETRTSFVEIG INGLSRDFIK IDLVKSPISY TAKRNDPSRS GLIYENGSDC
TNINPFSSRF FYYSNARIKE SLNQNQGTIH TLFNRNKEYQ SLIILSSSNC FRMGPFNNVK
YHNVIKESIK KDPPIPIRNL LGPLGTALKT ANFYPFSHLI TYNQILVINY LQLDNLKQTF
QVIKYFLMDE IGKIYNYDLC SNIILNPFNL NWYFLQHNYC EGMSTIMSLG QFICENVCIA
KNGPHLKSGQ VLIVKMDSVV IRSAKPYLAT PGATVHGHYG EILYEGDTLV TFIYEKSRSG
DITQGLPKVE QVLEVRSIDS ISMNLEKRVE GWHECITRTL GIPWGFLIGA ELTIVQSRIA
LVNKIQKVYR SQGVQIHNRH IEIIVRQITS KVLVSEDGMS NVFLPGELIG LLRAERMGRA
LEEAICYRAV LLGITKASLN TQSFISEASF QETARVLAKA ALRGRIDWLK GLKENVVLGG
MIPVGTGFEG LVHSSRQHTN LSLETKNNNI FEGEMRDILF HHRKFFDSCF SKNFHDTSEQ
SFIGIGFNDS