RPOC2_DIOEL
ID RPOC2_DIOEL Reviewed; 1382 AA.
AC A6MMJ7;
DT 04-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-JUL-2007, sequence version 1.
DT 03-AUG-2022, entry version 49.
DE RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324};
OS Dioscorea elephantipes (Elephant's foot yam) (Testudinaria elephantipes).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Dioscoreales; Dioscoreaceae;
OC Dioscorea.
OX NCBI_TaxID=145284;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=17644003; DOI=10.1016/j.ympev.2007.06.004;
RA Hansen D.R., Dastidar S.G., Cai Z., Penaflor C., Kuehl J.V., Boore J.L.,
RA Jansen R.K.;
RT "Phylogenetic and evolutionary implications of complete chloroplast genome
RT sequences of four early-diverging angiosperms: Buxus (Buxaceae),
RT Chloranthus (Chloranthaceae), Dioscorea (Dioscoreaceae), and Illicium
RT (Schisandraceae).";
RL Mol. Phylogenet. Evol. 45:547-563(2007).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC Rule:MF_01324}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR EMBL; EF380353; ABR01420.1; -; Genomic_DNA.
DR RefSeq; YP_001294342.1; NC_009601.1.
DR AlphaFoldDB; A6MMJ7; -.
DR PRIDE; A6MMJ7; -.
DR GeneID; 5236619; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Metal-binding;
KW Nucleotidyltransferase; Plastid; Transcription; Transferase; Zinc.
FT CHAIN 1..1382
FT /note="DNA-directed RNA polymerase subunit beta''"
FT /id="PRO_0000353559"
FT BINDING 224
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 294
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 301
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 304
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ SEQUENCE 1382 AA; 157124 MW; E9798B65F2F59B43 CRC64;
MGVFMTERAD LVFHNKVIDG TAMKRLISRL IDHFGMGYTS HILDQSKILG FQQATATSIS
LGIDDLLTIP SKEWLVQDTE QQRFILEKHY HYANVHAVEK LRQSIEIWYA TSEYLRQEMN
PNFRMTDPSN PVHLMSFSGA RGNASQVHQL VGMRGLMSDP QGQMIDLPIQ SNLREGLSLT
EYIISCYGAR KGVVDTAVRT SDAGYLTRRL VEVVQHIIVR RTDCGTIRSI SVSPRKGMTE
KIFVQTLIGR VLADDIYMGL RCIAVRKQEI GIGLANRLIT FRARPIYIRT PFTCRSTSWI
CQLCYGRSPT HGDLVELGEA VGIIASQSIG EPGTQLTLRT FHTGGVFTGG TAEHVRAPSS
GKIKFNEDLV HRTRTRHGHP AFLCSIGLYV TLEGRDIIHN LNIPPKGLIL VQNGQYVESE
QLIAEIRAGA FTLNFKERVR KHIYSESEGE MHWSTNVYHA PEYPYGNIHL LLKTSHLWIL
AGASRRSSIV SFSLHKDQDQ MNVHSFSVEE RYIYIFDLSM TNHRVKHKLL DTSGKKDREI
LEYSISDRII SNGHWNLISP SILQDNLDFL AKRRRNRFII PLQYDQEREK ELIPCFDISI
EIPINGILRG NSILAYFDDP VYRRSSSGIT KYGTLEVDSI VKKEDLIEYR GTKEFNQIQI
KVDRFFFIPE EVHILPGSSS IMVRNNSLIG VHTRLTLNTR SKIGGLVRVE RKKKSIELKI
FSGNIHFPGE TDKISRHSGI LIPPGTGKKN FNQSKKELKN WIYVQRITPT KKKYFVSVRP
VVTYEIADGI NLATLFPQDL LQEKDNVQLR VVNYILYGNG KSIRGIYHTS SQLVRTCLVL
NWDQEQNDSI EEVHTSFIEV RANDLIRDFI RIDLIKSTIS YTGKRNDTGS SGLIPDNGLN
STNINPFYSK SKIQSLTQDQ ETIGMGTLLN RNKECQSLII LSSSNCSQIG PFNGSKYKNV
TKESENIIPI MDFLGSLGTI VPKIANFYSS YHLITHNQIL LKRFLLLDNS KQTFQVLKYG
LIDENSRIYS PDPCSNIILN PFRLNWCFLR HNYCEETSTI ISLGQFFCEN ICLFKYGPNI
NIKKSGQIII VHVDSLVIRS AKPYLVTLGA TVHGHYGKIL YEGDRLVTFI YERSRSGDIT
QGLPKVEQVL EVRSIDSISM NLEKRVNSWN ECISRILGRP WGFMIGSELT IAQSRISLVN
RIQKVYRSQG VQIHNRHIEI IVRQVTSRVL VSEDGMSNVF SPGELIGLLR AERAGRALDE
AICYRAILLG ITRASLNTQS FISEASFQET TRVLAKAALR GRIDWLKGLK ENVVLGGIIP
VGTGFQKLVH RSRQDKNIHL EIKKNNLFDL EMRDILLHHR ELFCSCIQDN LYETSEQSFT
NS
