RPOC2_DRIGR
ID RPOC2_DRIGR Reviewed; 1381 AA.
AC Q06H07;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 31-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 50.
DE RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324};
OS Drimys granadensis.
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Magnoliidae; Canellales; Winteraceae; Drimys.
OX NCBI_TaxID=224735;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=17020608; DOI=10.1186/1471-2148-6-77;
RA Cai Z., Penaflor C., Kuehl J.V., Leebens-Mack J., Carlson J.E.,
RA dePamphilis C.W., Boore J.L., Jansen R.K.;
RT "Complete plastid genome sequences of Drimys, Liriodendron, and Piper:
RT implications for the phylogenetic relationships of magnoliids.";
RL BMC Evol. Biol. 6:77-77(2006).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC Rule:MF_01324}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR EMBL; DQ887676; ABH88287.1; -; Genomic_DNA.
DR RefSeq; YP_784376.1; NC_008456.1.
DR AlphaFoldDB; Q06H07; -.
DR PRIDE; Q06H07; -.
DR GeneID; 4363642; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Metal-binding;
KW Nucleotidyltransferase; Plastid; Transcription; Transferase; Zinc.
FT CHAIN 1..1381
FT /note="DNA-directed RNA polymerase subunit beta''"
FT /id="PRO_0000277189"
FT BINDING 224
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 296
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 303
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 306
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ SEQUENCE 1381 AA; 156784 MW; 78E52FCC2641B119 CRC64;
MEVLMAERAD LVFHNKVIDG TAMKRLISRL IDHFGMAYTS HILDQVKTMG FQQATATSIS
LGIDDLLTIP SKGWLVQDAE QQSLILEKHH HYGNVHAVEK LRQSIEIWYA TSEYLRQEMH
PNFRMTDPSN PVHIMSFSGA RGNASQVHQL VGMRGLMSDP QGQMIDLPIQ SNLREGLSLT
EYLISCYGAR KGVVDTAVRT SDAGYLTRRL VEVVQHIVVR RTDCGTIRGI SVSPRNGIGM
TEKMLIQTLI GRVLADDIYM GLRCIAARNQ DIGVGLVNRF IAFRAQSIYI RTPFICRSTS
WICRLCYGRS PTHGDLVELG EAVGIIAGQS IGEPGTQLTL RTFHTGGVFT GGTAEHVRAP
FNGKIKFNED LVHPTRTRHG HPAFLCYIDL YVTIESQDIL HNVNIPPKSF LLVQNDQYVE
SEQVIAEIRA RTSTFNFKER VRKHIYSDSE GEMHWSTDVY HAPEYTYGNV HLLPKTSHLW
ILSGGPRRSS LVPFSLHKDQ DQMNIHSLSV EQRESSDLSV TNDRARHKLF SSDPSGKKEG
KILDYSGPAR IISNGHWNFI YPAILHENSY LLAKRRRNRF IIPFQYDQER EKELMPRSGI
SIEIPINGIL RRNSILAYFD DPRYRRSSSG ITKYGTVEVD SIVKKEDLIE YRGAKEFSPK
YQMKVDRFFF IPEEVHILPG SSSIMVRNNS IIGVDTRITL NTRSRIGGLV RVERKKKRIE
LKIFSGDIHF PGEADKISRH SGILIPPGTG KKNSKESKKL QNWIYVQRIT PTKKKYFVSV
RPVVTYEIAD GINLATLFPQ DLLQERDNVK FRVVNSILYR NGKPIRGIYY TSIQLVRTCL
VLNWDQDRNG SIEKVKASIV EVRANDLIRD FIRIDLVKSP ISYTGKRNDM AGSGLIPDNG
SDHTNINPFY SKVRRIQSLT QHQGTIRTLL NRNKECQSFL ILSSSNCSRI GPFNGSKSHN
VTKESIQIKE DPMIPIRNSL GPLGTVPKIA NFDSPYYLIT HNQILLNKYL LLDNLKQTFQ
VLKYYLMDEN GRIYNPYPCR NIIFHPFDLT WCFLHHDYCE KTSTIIVLGQ FICENENVCI
SKYGPQIKSG QVLIVHVDSL VIRSAKPHLA TPGATVHGHY GEILYEGDTL VTFIYEKSRS
GDITQGLPKV EQVLEVRSVD SISMNLEKRV EGWNEHIKRI LGIPWGFLIG AELTIAQSRI
SLVNKIQKVY RSQGVQIHNR HIEIIVRQIT SKVLVSEDGM SNVFSPGELI GLLRAERTGR
SLEEAICYRA ILWGITRASL NTQSFISEAS FQETARVLAK AALRGRIDWL KGLKENVVLG
GMIPVGTGFK GLVHRSRQDN NIPLEIKKKN LFEGEIRDIL FHHRELFGSC IPNNFHNTPE
Q
