RPOC2_EMIHU
ID RPOC2_EMIHU Reviewed; 1267 AA.
AC Q4G3A5;
DT 07-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT 30-AUG-2005, sequence version 1.
DT 03-AUG-2022, entry version 55.
DE RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324};
OS Emiliania huxleyi (Coccolithophore) (Pontosphaera huxleyi).
OG Plastid; Chloroplast.
OC Eukaryota; Haptista; Haptophyta; Prymnesiophyceae; Isochrysidales;
OC Noelaerhabdaceae; Emiliania.
OX NCBI_TaxID=2903;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CCMP373 / CSIRO-CS-57 / BT6;
RX PubMed=16303746; DOI=10.1093/dnares/12.2.151;
RA Sanchez-Puerta M.V., Bachvaroff T.R., Delwiche C.F.;
RT "The complete plastid genome sequence of the haptophyte Emiliania huxleyi:
RT a comparison to other plastid genomes.";
RL DNA Res. 12:151-156(2005).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC Rule:MF_01324}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR EMBL; AY741371; AAX13861.1; -; Genomic_DNA.
DR RefSeq; YP_277362.1; NC_007288.1.
DR AlphaFoldDB; Q4G3A5; -.
DR PRIDE; Q4G3A5; -.
DR GeneID; 3562438; -.
DR Proteomes; UP000013827; Unassembled WGS sequence.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 4.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Metal-binding;
KW Nucleotidyltransferase; Plastid; Reference proteome; Transcription;
KW Transferase; Zinc.
FT CHAIN 1..1267
FT /note="DNA-directed RNA polymerase subunit beta''"
FT /id="PRO_0000225332"
FT BINDING 222
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 290
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 297
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 300
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ SEQUENCE 1267 AA; 142493 MW; 58BD3CE6C8BF32B4 CRC64;
MQPDFKKIHS FENNLINKRS LKDLMYQAFL NYGIVKSSII ADRVKNLTFH FATQSGVSLS
VEDLRVPAKK RELIGLTRNE VETTQKRYEI GSITSVEKFQ KTIDIWNNAN NFLKDDVLTY
FRESDPLNPL YIMAFSGARG NISQVRQLVG MRGLMSNPQG QIIDLPIKSN FREGLTVTEY
IISSYGARKG LVDTALRTAD SGYLTRRLVD VAQDIIVRES DCGTTEGLWA TELASNNFLN
LRGRLLAEPI FTQEGNLFAP ANTEITETFL QTLKTLKDHS PIKVRSSLTC TSTRSVCQNC
YGWHLSHSKL VDLGEAVGIV AAQSIGEPGT QLTMRTFHTG GVFSGDLTRQ VRSPMEGKIE
YWEGHKASLF RTMHGKMGFR LKEEVNLIIK NSFGTTISFE IPAESLLLVN TDQYVYENEI
IAEIQKDTNL ILEEEQKEIY SETSGEVFFN ALDVKVQADK QGNTYKTNNK AGLIWILQGS
FYELPAFSET LLKPGLNLSK NTLLSRTPLY NKYPGWVQID KSDNGTGICV QNFSVTLTNA
FINEKVENSS RLQIKTDNQD FQFQLTTEPN SVLKQGDTIA VLDDNTYRTT TGGIVTYSLE
NPQASKKRKS VKNLFKGYFY WIPEETFKFS TLDEVRLSFS KNESIVGVGE EIMPNTFSKV
GGFFQIKEAE QEVTIKPGEL FELTSAESQI FDKSDRFVKP GNFIVPGKII TQQLVYLEFF
ELNEKQYILA RPVQVFEVPK EEDLSLSQSF FPYNSHEHIK LKVIKRVFYK NWEKIISNNG
INLLQTFIVF DFKQEQQGLE PRLEFTPSKV PGKSFLKIAL YEVIKTFENN RKSSHSSLPT
TTRNFVSNKQ YVASNTLIGQ IETTAGLVNK LAAINPHLNS GNMKEVLILN PSDLKKVFCP
NVELLKKVSV GDLVRIGTLL NDNVKSPYSG QILEIFEDHI LIRLSQPYLI SAGTILHATS
NNLVKAGDIL ATLVYETIKT TDIVQGLPKV EELLEARKVL HNALLAPCPG YAYVRFNKHG
ESTVQLIGLD NEVQTLALKP GIKTKFNSGD FVEVSSALTD GLISPHTKLE TLFSYFKNRY
TSFEACKLSF KLLQLFLIGE IQRTYRSQGV DIADKHVELI VKQMTSKVCI EDSGTTTFLP
GEVLNFQKMA DIALVAENKG EIPPSYVPLL LGITKASLNS DSFISAASFQ ETTRVLTEAA
IEGRKDWLTG LKENVIIGRL IPAGTGFNYL ENQERLAREK GEIDATFHRE AKPLSENILD
LRLVKKE
