RPOC2_EUCGG
ID RPOC2_EUCGG Reviewed; 1392 AA.
AC Q49L08;
DT 07-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2005, sequence version 1.
DT 03-AUG-2022, entry version 51.
DE RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324};
OS Eucalyptus globulus subsp. globulus (Tasmanian blue gum).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Myrtales; Myrtaceae; Myrtoideae; Eucalypteae; Eucalyptus.
OX NCBI_TaxID=71271;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16303753; DOI=10.1093/dnares/dsi006;
RA Steane D.A.;
RT "Complete nucleotide sequence of the chloroplast genome from the Tasmanian
RT blue gum, Eucalyptus globulus (Myrtaceae).";
RL DNA Res. 12:215-220(2005).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC Rule:MF_01324}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR EMBL; AY780259; AAX21019.1; -; Genomic_DNA.
DR RefSeq; YP_636289.1; NC_008115.1.
DR AlphaFoldDB; Q49L08; -.
DR SMR; Q49L08; -.
DR GeneID; 4108360; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Metal-binding;
KW Nucleotidyltransferase; Plastid; Transcription; Transferase; Zinc.
FT CHAIN 1..1392
FT /note="DNA-directed RNA polymerase subunit beta''"
FT /id="PRO_0000225333"
FT BINDING 224
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 295
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 302
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 305
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ SEQUENCE 1392 AA; 158502 MW; 09142D85AD2D4D17 CRC64;
MEVLMAERVN LVFHNKVIDG TAIKRLISRL IDHFGMAYTS HILDQVKTLG FQQATATSIS
LGIDDLLTIP SKGWLVQDAE QQSLILEKHH HYGNVHAVEK LRQSIEVWYA TSEYLRQEMN
PNFRMTDPFN PVHIMSFSGA RGNASQVHQL VGMRGLMSDP QGQMIDLPIQ SNLREGLSLT
EYIISCYGAR KGVVDTAVRT SDAGYLTRRL VEVVQHIVVR RTDCGTIRGV SVSPRNGMMP
ERIFIQTLIG RVLADDIYMG PRCIAIRNQD IGIGLVNRFI TFRTQPISIR TPFTCRSTSW
ICRLCYGRSP THGDLVELGE AVGIIAGQSI GEPGTQLTLR TFHTGGVFTG GTAEHVRAPS
NGKIKFNDDL VHPTRTRHGH PAFLCSIDLY VTIESEDILH NVTIPPKSFL LVQNNQYVES
EQVIAEIRAG TYTFNLKERV RKHIYSESEG EMHWSTDVYH APEFTYSNVH LLPKTSHLWI
LSGGSCRSSR VPFSLYKDQD QMNLRSTERE RRYISSLSVN NDQMRYELCS SDFSGKIKED
RIPDYSELNR IISIVHCNLK YPTTFDENSD LLAKRRRNRF LIPLQSIQER KKELMPHSGI
SIELPINGIL RRNSILAYFD DPRYRRKSSG IIKYGTLGVH SVVKKEDLIE YRGIKEFKQK
CQMKLDPFFF IPEEVHIFPE SSSIMVRNNS LIGVDTRIAL NTRSRVGGLV RVERKKKRIE
LKIFSGDIHF PGETDKISRH SGILIPPGTG KTNFKESKKW KNWIYVQRIT PTKKKYFVLV
RPVVTYEVLD GINLATLFPP DLLQERDNMQ LRVVNYIVYR NGKPIRGISD TSIQLVRTCL
ILNWDQDKKS SSIEEAHTSF VEVSTNGLIR DFLRIDLVKF PISYLRKRND PSGSGLISDS
DNVSDHTNSN PFYSKTKIQQ LLSQNQGTIR TLLNKNKECP SLIILSSSNC FRMGPFNAGK
YHNVIKESIK KDPIIKIRNS IGPLGTVLQF VNFYSFYYLI THNPILVTKY LQLENLKQTF
QVINYYLMDE NGRILNPDSC SNIVLNSFNL NWYFLHHNYY HNYFEERSTI ISLGQFICEN
VCISKNGPHL KSGQVLIVQV DSVVIRSAKP YLATPGATVH GHYGEILYEG DTLVTFIYEK
SRSGDITQGL PKVEQVLEVR SIDSISMNLE KRIEGWNEHI KKILGIPWGF LIGAELTIVQ
SRISLVNKIQ KVYRSQGVQI HNRHIEIIVR QITSKVLVSE DGMSNVFLPG ELIGLLRAAR
TGRALEESIC YRAILLGITR ASLNTQSFIS EASFQETARV LAKAALRGRI DWLKGLKENV
VIGGMIPVGT GFKGLVHRSR QHKNIPLKTK KKNFFEGEIG DILFHHRELF DSSISKKFHD
TSEQSFRGFN DS
