RPOC2_EUGGR
ID RPOC2_EUGGR Reviewed; 830 AA.
AC P23581;
DT 01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1991, sequence version 1.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=DNA-directed RNA polymerase subunit beta'';
DE EC=2.7.7.6;
DE AltName: Full=PEP;
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta'';
DE Short=RNA polymerase subunit beta'';
GN Name=rpoC2;
OS Euglena gracilis.
OG Plastid; Chloroplast.
OC Eukaryota; Discoba; Euglenozoa; Euglenida; Spirocuta; Euglenophyceae;
OC Euglenales; Euglenaceae; Euglena.
OX NCBI_TaxID=3039;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Z / UTEX 753;
RX PubMed=2110656; DOI=10.1093/nar/18.7.1869;
RA Yepiz-Plascencia G.M., Radebaugh C.A., Hallick R.B.;
RT "The Euglena gracilis chloroplast rpoB gene. Novel gene organization and
RT transcription of the RNA polymerase subunit operon.";
RL Nucleic Acids Res. 18:1869-1878(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Z / UTEX 753;
RX PubMed=8346031; DOI=10.1093/nar/21.15.3537;
RA Hallick R.B., Hong L., Drager R.G., Favreau M.R., Monfort A., Orsat B.,
RA Spielmann A., Stutz E.;
RT "Complete sequence of Euglena gracilis chloroplast DNA.";
RL Nucleic Acids Res. 21:3537-3544(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 586-830.
RC STRAIN=Z / UTEX 753;
RX PubMed=6277930; DOI=10.1016/s0021-9258(19)81105-5;
RA Orozco E.M., Hallick R.B.;
RT "Euglena gracilis chloroplast transfer RNA transcription units. II.
RT Nucleotide sequence analysis of a tRNAVal-tRNAAsn-tRNAArg-tRNALeu gene
RT cluster.";
RL J. Biol. Chem. 257:3265-3275(1982).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000250|UniProtKB:P0A8T7};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000250|UniProtKB:P0A8T7};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000250|UniProtKB:P0A8T7};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC subfamily. {ECO:0000305}.
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DR EMBL; X17191; CAA35054.1; -; Genomic_DNA.
DR EMBL; X70810; CAA50136.1; -; Genomic_DNA.
DR EMBL; M22010; AAA84228.1; -; Genomic_DNA.
DR PIR; S19259; RNEGB2.
DR RefSeq; NP_041949.1; NC_001603.2.
DR AlphaFoldDB; P23581; -.
DR GeneID; 807500; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 4.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Metal-binding;
KW Nucleotidyltransferase; Plastid; Transcription; Transferase; Zinc.
FT CHAIN 1..830
FT /note="DNA-directed RNA polymerase subunit beta''"
FT /id="PRO_0000067923"
FT BINDING 219
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:P0A8T7"
FT BINDING 291
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:P0A8T7"
FT BINDING 298
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:P0A8T7"
FT BINDING 301
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000250|UniProtKB:P0A8T7"
FT CONFLICT 593
FT /note="I -> II (in Ref. 3; AAA84228)"
FT /evidence="ECO:0000305"
FT CONFLICT 688
FT /note="L -> R (in Ref. 3; AAA84228)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 830 AA; 94757 MW; BEF9A964B70F68B8 CRC64;
MNFGNIVCFN RVFDKNEIRN LISWFLSNYG SIRTKELLDK MKNFGFTYAT TTGLSLGLGD
LKIPSSKANL VKSSEKILFK TRNRYKNGMI NFINVLDEER DIWNVVNENL KKESINNLRQ
SDFLNPLYSM TLSGARGSIT QVKQLIGMRG LMSDSQGNVI PFPIKTNFKE GLNITEYFVS
CYGARKGLID TALKTANAGY LTRRMIYTAQ NLIVKRSDCF TKYNTCVLLQ NQDKEELKFL
KEKLIGRVLA KTIVNAKTGD ILVSAGQDIC NYTFKKIINF PEVYIRTPFN CVLMEGICQI
CYGWNLACGK MVELGECVGI LAAQSIGEPG TQLTMRTFHT GGVFSAKAKE VITSPLNGKI
WYDLNTGLRR VYNKFKEKAF LTLQEKKIVI YENDVSKSIM FLPSSTLLYV KPGRKIFDKQ
IIGESVNSNL KNDVFGIEEI RDVKAKISGQ IFFPQINSKQ FGKIFWIISS IIMSFTSLFF
HLTKKFSFKN KLICPTTNVH KKELFVNRKK ELFPRKFGKL FINIRNVNSL VDKSKVLKKR
SSHIITCILD QDKVIMLRNL KKQKRIFGNF KIGCFLKTGQ IISNFKLLHS SQIIQERKEF
SIFRKVMPFS TNDDTLMRIE DKPFIRKNQL LYRVNFVREK TYDIVQGLPK VEKLLEARMT
SSLKEIINNP HDILTESFFT FLDDYENLVA ARKSFEVIQK YLIDGVQTVY KSQGVKIADK
HIELIVKQIT SKVIVTNPGD SSFMVGDFLD LNLVEVLNKR LVNSIVYEPI IMGLTRFSLS
SQSFIAQASF QETTRVLTKA ALQGRADWLS GLKENLVLGN IIPAGTGFKN
