RPOC2_FAGEA
ID RPOC2_FAGEA Reviewed; 1365 AA.
AC B2XWN9;
DT 04-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-2008, sequence version 1.
DT 03-AUG-2022, entry version 43.
DE RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324};
OS Fagopyrum esculentum subsp. ancestrale (Wild buckwheat).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC Caryophyllales; Polygonaceae; Polygonoideae; Fagopyreae; Fagopyrum.
OX NCBI_TaxID=180217;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=18492277; DOI=10.1186/1471-2229-8-59;
RA Logacheva M.D., Samigullin T.H., Dhingra A., Penin A.A.;
RT "Comparative chloroplast genomics and phylogenetics of Fagopyrum esculentum
RT ssp. ancestrale - a wild ancestor of cultivated buckwheat.";
RL BMC Plant Biol. 8:59-59(2008).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC Rule:MF_01324}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR EMBL; EU254477; ABY79722.1; -; Genomic_DNA.
DR RefSeq; YP_001936507.1; NC_010776.1.
DR AlphaFoldDB; B2XWN9; -.
DR SMR; B2XWN9; -.
DR GeneID; 6336004; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Metal-binding;
KW Nucleotidyltransferase; Plastid; Transcription; Transferase; Zinc.
FT CHAIN 1..1365
FT /note="DNA-directed RNA polymerase subunit beta''"
FT /id="PRO_0000353561"
FT BINDING 224
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 295
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 302
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 305
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ SEQUENCE 1365 AA; 154900 MW; E1511AF02D1E582A CRC64;
MEVLMAKGAN LVFHNKVIDG IAMKRLISRF IDHFGMAYTS HILDQVKTLG FQQATATSIS
LGIDDLLTIP SKGWLVQDAE QQSFILEKQH HYGNIHAVEK LRQSIEIWYA TSEHLRQEMN
PNFRMTDPYN PVHMMSFSGA RGNASQVHQL VGMRGLMSDP QGQMIDLPIQ SNLREGLSLT
EYIISCYGAR KGVVDTAVRT SDAGYLTRRL VEVVQHVVVR RRDCGTTRGI SVSPQTGTIP
EILFIQTLIG RVLADDIYKG PRCIAARNQD IGVGLVNRLI TFRVQTISIR TPFTCRSTSW
ICRLCYGRSP THGDLVELGE AVGIIAGQSI GEPGTQLTLR TFHTGGVFTG GTAVHVRAPS
NGKIKFNEDL VHPTRTRHGH PAFLCYIDLA VTIESEDIIH NVNIPPKSFL LVQKDQYVES
EQVIAEIRAG TSTLNFKEKV RKHIYSDSQG EMHWSTDVDH APEFTYGNVH LLPKTSHLWI
LAGRPHKSRV ATRSLHKDQD QTNAHFLSVE QRYISNSSVI NAYMRHTFFS SDLSSTNSNK
GDSISDYSER KRIVRTRHSN LIYPAKNSYL LGKRRRNRFM TTFQSIQELE KELRAPSGIS
IDIPLNGSFR RNSILAYFDD PRYRRESAGI TKYGTIEAHS IKKKEGLIEY RGVNEFRAKY
QMQVDRFFFI PEEVHILPRS SAIMVRNNSI IGVDTQITLT TRSRVGGLVR VEKKKKKIGL
KIFSGNIHFP GKTDKISRHR GILIPPGRGQ TKLKNWSYVQ RITPTKKRYF VLIRPAITYE
ITDGISLSTL FPQDLLQERD KVQLRVVNYI LSGNGKPIRG ISDTSIQLVR TCLVLNWDHD
KKSSSIEESR ASFVEVKTND MMRDFLRIDL VKSTISYAGK RNDPSGSGLL SDNESDRMNP
FYSIYIYSKA RLQQPFSQNN ETVRTLLNRT KEFQSFIILS SANCFRMGPF KGLKYYKELD
PIIPIKNSSG PFGTSLQIAN FYSFYRLITQ DKILVTNYLQ LDNLKQTFWV MKYYFIDDNR
KIYNPDPVSN IVLNSFKLNW YCLHHNYYEE TSARMGLGQF LCENVCLAKN GPRLKAGQVL
IVQVDSVVIR AAKPYLATPG ATVHGHYGEI IYEGDTLVTF IYEKSRSGDI TQGLPKVEQV
LEVRSLESIS MNLERRIDAW NERITGILGL PWGFVVGAEL TIVQSRISLV NKIQKVYRSQ
GVQIHNRHLE IIVHQITSKV LVSEDGMSNV FLPGELIGLL RAERTGRALE EVISYRAVLV
GITRASLNTH SFISEASFQE TARVLAKAAL RGRIDWLKGL KENVVLGGKI PVGTGFKGLV
HPSSQHKSIP LKNKKKNLFE GEINDILFYY REFFNIEFLC FKNYQ
