ID   RPOC2_GALSU             Reviewed;          85 AA.
AC   P35018;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   03-AUG-2022, entry version 76.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta'';
DE            EC=2.7.7.6;
DE   AltName: Full=PEP;
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta'';
DE            Short=RNA polymerase subunit beta'';
DE   Flags: Fragment;
GN   Name=rpoC2;
OS   Galdieria sulphuraria (Red alga).
OG   Plastid; Chloroplast.
OC   Eukaryota; Rhodophyta; Bangiophyceae; Cyanidiales; Cyanidiaceae; Galdieria.
OX   NCBI_TaxID=130081;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=14-1-1 / Isolate 107.79/Goettingen;
RX   PubMed=8219057; DOI=10.1007/bf00021420;
RA   Kostrzewa M., Zetsche K.;
RT   "Organization of plastid-encoded ATPase genes and flanking regions
RT   including homologues of infB and tsf in the thermophilic red alga Galdieria
RT   sulphuraria.";
RL   Plant Mol. Biol. 23:67-76(1993).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6;
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC       subfamily. {ECO:0000305}.
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DR   EMBL; X67814; CAA48017.1; -; Genomic_DNA.
DR   AlphaFoldDB; P35018; -.
DR   SMR; P35018; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR   PANTHER; PTHR19376; PTHR19376; 1.
PE   3: Inferred from homology;
KW   Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW   Transcription; Transferase.
FT   CHAIN           <1..85
FT                   /note="DNA-directed RNA polymerase subunit beta''"
FT                   /id="PRO_0000067924"
FT   NON_TER         1
SQ   SEQUENCE   85 AA;  10115 MW;  DB038EE3763E21B8 CRC64;
     SFQETRRVLT KAAFENHIDW LKGLKENVII GRLIPAGTGF KQFYLYEYTK KNCEENIISL
     DPYNFEDNII YKILTNQLEQ NKRLN