RPOC2_GOSBA
ID RPOC2_GOSBA Reviewed; 1391 AA.
AC A0ZZ25;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 1.
DT 03-AUG-2022, entry version 46.
DE RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324};
OS Gossypium barbadense (Sea-island cotton) (Egyptian cotton).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Malvales; Malvaceae; Malvoideae; Gossypium.
OX NCBI_TaxID=3634;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=17159292; DOI=10.1266/ggs.81.311;
RA Ibrahim R.I.H., Azuma J., Sakamoto M.;
RT "Complete nucleotide sequence of the cotton (Gossypium barbadense L.)
RT chloroplast genome with a comparative analysis of sequences among 9 dicot
RT plants.";
RL Genes Genet. Syst. 81:311-321(2006).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC Rule:MF_01324}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR EMBL; AP009123; BAF41237.1; -; Genomic_DNA.
DR RefSeq; YP_913177.1; NC_008641.1.
DR AlphaFoldDB; A0ZZ25; -.
DR GeneID; 4575247; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Metal-binding;
KW Nucleotidyltransferase; Plastid; Transcription; Transferase; Zinc.
FT CHAIN 1..1391
FT /note="DNA-directed RNA polymerase subunit beta''"
FT /id="PRO_0000277190"
FT BINDING 220
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 291
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 298
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 301
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ SEQUENCE 1391 AA; 158398 MW; FA904D9F706D48A5 CRC64;
MAERANLVFH NKVIDGTAIK RLISRLIDHF GMAYTSHILD QVKALGFQQA TATSISLGID
DLLTIPSKGW LVQDAEQQSL ILEKHHHFGN VHAVEKLRQS IEIWYATSEY LRQEMNPNFR
MTDPFNPVHI MSFSGARGNA SQVHQLVGMR GLMSDPQGQM IDLPIQSNLR EGLSLTEYII
SCYGARKGVV DTAVLTSDAG YLTRRLVEVV QHIVVRRTDC GTTRGISVSP QKRTLPERIF
IQTLIGRVLA DDIYMGPRCI AIRNQDIGLG LVDRFRAFRT QPISIRTPFT CRSTSWICRL
CYGRSPTHGD LVELGEAVGI IAGQSIGEPG TQLTLRTFHT GGVFTGGTAE HVRAPFNGKI
KFNEDLVHPT RTRHGHPAFL CYRDLYVIIE SEDIIHKVTI PPKSFLLVQN DQYVESEQVI
AEIRAGTYTL NLKERVRKHI YSDSEGEMHW STDVYHSPEY TYSNVHLLPK TSHLWILSGG
SYKFSVVPFS LHKDQDQINI HYLSAERRYI SRFSVNNDQV RHNLFSSDFS DEKEERIYDY
SELNRIIGTG HCDFIYSAIL HENADLLAKR RRNRFIIPFQ LIQDQEKELM LHSHSGISME
IPINGIFRRK GILAFFDDPR YRRKSSGITK YGTLGAHSIV KREDVIEYRG VKKVKPKYQM
KVDRFFFIPE EVHILSESSS IMVRNNSIIG VDTPITLNTR SQVGGLVRVE RKKKRIELKI
FSGNIYFPGE RDKISRHSGI LIPPGTGKTN SKESKKLKNW IYVQRITPTK KKYFVLVRPV
TPYEIPDGLN LATLFPQDPF QEKDNMQLRA VNYILYGNGK PTRRISDTSI QLVRTCLVLS
WDQDNKSSFA EEVCASFVEV RTNGLIRDFL RIDLVKSHIF YIRKRNDPSG SELISDNRSD
RTNKNPFYSI YSNARIQQSF SQNHGTIHTL LNRNKESQSL IILSASNCFR MGPFNDVKYH
NVIKQSIKKD PLIPIKNLLG PLGTAPKIAN FYSSFYPLIT HNQTSVAKYF ELDNLKQAFQ
VLNYYLIAEN GRIYNFDPCR NIFLNAVNLN WYFPHHHYNY CEETSTIISL GQFICENVCI
AKSGPRLKSG QVFIVQADSI VIRSAKPYLA TPGATVHGHY GETLYEGDTL VTFIYEKSRS
GDITQGLPKV EQVLEVRSID SISMNLEKRI EGWNECITRI LGIPWGFVIG AELTIVQSRL
SLVNKIQKVY RSQGVQIHNR HIEIIVRQIT SKVLVSEDGM SNVFLPGELI GLLRAERTGR
ALEEAICYRA VLLGITRASL NTQSFISEAS FQETARVLAK AALRGRIDWL KGLKENVVLG
GMIPAGTGFK GLVHRSRQHN NILLETKKKN FFGGEMRDIF FHHRELFDSC FSNNLHDTSG
RSFIGIEFND S
