RPOC2_GUIAB
ID RPOC2_GUIAB Reviewed; 1381 AA.
AC B2LMI2;
DT 04-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT 10-JUN-2008, sequence version 1.
DT 03-AUG-2022, entry version 41.
DE RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324}; OrderedLocusNames=GuabCp010;
OS Guizotia abyssinica (Niger) (Ramtilla).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; campanulids; Asterales; Asteraceae; Asteroideae;
OC Heliantheae alliance; Millerieae; Guizotia.
OX NCBI_TaxID=4230;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. PI 508077;
RA Kane N.C., Dempewolf H., Stewart M.L., Cronk Q., Rieseberrg L.H.;
RT "Guizotia abyssinica chloroplast sequenced using Solexa.";
RL Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC Rule:MF_01324}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; EU549769; ACB86516.1; -; Genomic_DNA.
DR RefSeq; YP_001837349.1; NC_010601.1.
DR AlphaFoldDB; B2LMI2; -.
DR GeneID; 6219084; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Metal-binding;
KW Nucleotidyltransferase; Plastid; Transcription; Transferase; Zinc.
FT CHAIN 1..1381
FT /note="DNA-directed RNA polymerase subunit beta''"
FT /id="PRO_0000353562"
FT BINDING 224
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 295
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 302
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 305
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ SEQUENCE 1381 AA; 156795 MW; 5C87621A39C122AE CRC64;
MEVLMAERPT QVFHNKVIDG TAMKRLISRF IDHYGIGYTS HILDQVKTLG FRQATAASIS
LGIDDLLTIP SKRWLVQDAE QQSFILEKHH HYGNVHAVEK LRQSIEIWYA TSEYLRQEMT
PNFRMTDPFN PVHIMSFSGA RGNASQVHQL VGMRGLMSDP QGQMIDLPIQ SNLREGLSLT
EYIISCYGAR KGVVDTAIRT SDAGYLTRRL VEVVQHIVVR RTDCGTVRGI SVSPRNGMMA
DRIFIQTLIG RVLADDIYIG SRCIATRNQD IGVGLVNRFI TFRAQPISIR TPFTCRSTSW
ICQLCYGRSP AHDDLVELGE AVGIIAGQSI GEPGTQLTLR TFHTGGVFTG GTAEHVRAPS
NGKIKFNEDL VHPTRTRHGH PAFLCSRDLY VTIESEDIIH NVCIPPKSFL LVQNDQYVES
EQVIAEIRAR TSTLNLKEKV RKHIYSDSEG EMHWNTDVYH APEFTYGNIH LLPKTSHLWI
LLGEPWRSSL GPCSIHKDQD QMNAYSLSVK RRYISNPSVT NNQVRHKFFS SYFSGKNQKG
DRIPDYSELN RMTCTGRCNL RYPGILDGNS DLLAKRRRNR VIIPLDSIQE GENQLIPSSG
ISIEIPRNGI LRRNSILAYF DDPRYIRKSS GLTKYETREL NSIVNEEDLI EYRGVKVFWP
KYQKEVNPFF FIPVEIHILA ESSSIMVRHN SIIGVDTQIT LNRRSRVGGL VRVKKKAEKI
KLIIFSGDIH FPEKTNKAFR LIPPGGGKKN SKEYKKLKNW LYIQRMKLSR YEKKYFVLVQ
PVVPYKKTDG INLGRLFPAD LLQESDNLQL RVVNYILYYD PILEIWDTSI QLVRTCLVLN
WDQDKKIEKA CASFVEIRTN GLIRDFLRID LAKSPISYTG KRNDLPGSGL IYENGSDRAN
VNPFSSIYSS SKARIQESLN PNQGTIHTLL NRNKESQSLI ILSSSNCSRI GPFNDVKSPN
VIKESIKKDP LIPIRNSLGP LGTGFPISNF DLFSHLITHN KILVTNYLQL DNLKHIFQIL
KYYLLDENGK IYNPYSCSNI ILNPFNLNWY FLHYNYCEET STIVSLGQFL CENVCIAKKG
PHLKSGQVLI LQVDSVVIRS AKPYLATPGA TVHGHYGEIL YEGDTLVTFI YEKSRSGDIT
QGLPKVEQVL EVRSIDSISM NLEKRIEGWN KCITRILGIP WAFFIGAELT IVQSRISLVN
KVQKVYRSQG VQIHNRHIEI IVRQITSKVL VSEDEMSNVF SPGELIGLLR AERMGRALEE
AICYQAVLLG ITRASMNTQS FISEASFQET ARVLAKAALL GRIDWLKGLK ENVVLGGMIP
VGSGFKTPSS EPNNIPNNIA FELKKENLLE GEMKDILFYH RKFFDSCLSK NFHDTQEQSF
F
