RPOC2_HELAN
ID RPOC2_HELAN Reviewed; 1362 AA.
AC Q1KXX0;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2006, sequence version 1.
DT 03-AUG-2022, entry version 55.
DE RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324};
OS Helianthus annuus (Common sunflower).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; campanulids; Asterales; Asteraceae; Asteroideae;
OC Heliantheae alliance; Heliantheae; Helianthus.
OX NCBI_TaxID=4232;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. HA383;
RA Timme R.E., Kuehl J.V., Boore J.L., Jansen R.K.;
RT "A comparison of the first two published chloroplast genomes in Asteraceae:
RT Lactuca and Helianthus.";
RL Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC Rule:MF_01324}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR EMBL; DQ383815; ABD47135.1; -; Genomic_DNA.
DR RefSeq; YP_588106.1; NC_007977.1.
DR AlphaFoldDB; Q1KXX0; -.
DR GeneID; 4055574; -.
DR KEGG; han:4055574; -.
DR OrthoDB; 731145at2759; -.
DR PhylomeDB; Q1KXX0; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Metal-binding;
KW Nucleotidyltransferase; Plastid; Transcription; Transferase; Zinc.
FT CHAIN 1..1362
FT /note="DNA-directed RNA polymerase subunit beta''"
FT /id="PRO_0000277192"
FT BINDING 224
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 295
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 302
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 305
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ SEQUENCE 1362 AA; 154464 MW; 0083CA88CE236A78 CRC64;
MEVLMAERPT QVFHNKVIDG TAMKRLISRF IDHYGIGYTS HILDQVKTLG FRQATAASIS
LGIDDLLTIP SKRWLVQDAE QQSVLLEKHH HYGNVHAVEK LRQSIEIWYA TSEYLRQEMT
PNFRMTDPFN PVHIMSFSGA RGNASQVHQL VGMRGLMSDP QGQMIDLPIQ SNLREGLSLT
EYIISCYGAR KGVVDTAIRT SDAGYLTRRL VEVVQHIVVR RTDCGTVRGI SVSPRNGMMA
DRIFIQTLIG RVLADDIYIG SRCIATRNQD IGVGLVNRFI TFRAQPISIR TPFTCRSTSW
ICQLCYGRSP AHDDLVELGE AVGIIAGQSI GEPGTQLTLR TFHTGGVFTG GTAEHVRAPF
NGKIKFNEDL VHPTRTRHGH PAFLCSRDLY VTIESEDIIH NVCIPPKSFL LVQNDQYVES
EQVIAEIRAR TSTLNLKEKV RKHIYSDSEG EMHWNTDVYH APEFTYGNIH LLPKTSHLWI
LLGEPWRSSL GPCSIHKDQD QMNAYSLSVK RRYISNPSVT NNQVRHKFFS SYFSGKNKKG
DRIPDYSELN RMTCTGRCNL RYPGILDGNS DLLAKRRRNR VIIPLDSIQE GENQLIPSSG
ISIEIPRNGI LRRNSILAYF DDPRYIRKSS GLTKYETREL NSIVNEENLV EYRGVKVFWP
KYQKEVNPFF FIPVEVHILA ESSSIMVRHN SIIGVDTQIT LNRRSRVGGL VRVKKKAEKI
KLIIFSGDIH FPGKTNKAFR LIPPGGGKQN SKEYKKLKNW LYIQRMKLSR YEKKYFVLVQ
PVVPYKKTDG INLGRLFPAD LLQESDNLQL RVVNYILYYD PILEIWDTSI QLVRTCLVLN
WDQDKKIEKA CASFVEIRTN GLIRDFLRID LAKSPISYTG KRNDLPGSGL ISENGSDRAN
GNPFSSIYSY SKATIQESLN PNQGTIHTLL NRNKESQSLI ILSSSNCSRI GPFNDVKYPN
VIKDSIKKDP LIPIRNSLGP LGTGFPIYHF DLFSHLITHN QILVTNYLQL DNLKQIFQIL
KYYLLDENGK IYNPYSCSNI ILNPFKLNWY FLHYNYCEET STIVSLGQFL CENVCIAKKG
PHLKSGQVLI LQVDSVVIRS AKPYLATPGA TVHGHYGEIL YEGDTLVTFI YEKSRSGDIT
QGLPKVEQVL EVRSIDSISM NLEKRIEGWN KCITRILGIP WAFFIGAKLT IVQSRISLVN
KVQKVYRSQG VQIHNRHIEI IVRQITSKVL VSEDEMSNVF SPGELIGLLR AERMGRALEE
AICYQAVLLG ITRASMNTQS FISEASFQET ARVLAKAALL GRIDWLKGLK ENVVLGGMIP
VGSGFKTPSS EPNNIPNNIA FELKKKNLLE GEMKDILFYH RK
