RPOC2_ILLOL
ID RPOC2_ILLOL Reviewed; 1385 AA.
AC A6MMT4;
DT 04-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-JUL-2007, sequence version 1.
DT 03-AUG-2022, entry version 53.
DE RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324};
OS Illicium oligandrum (Star anise).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Austrobaileyales; Schisandraceae; Illicium.
OX NCBI_TaxID=145286;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=17644003; DOI=10.1016/j.ympev.2007.06.004;
RA Hansen D.R., Dastidar S.G., Cai Z., Penaflor C., Kuehl J.V., Boore J.L.,
RA Jansen R.K.;
RT "Phylogenetic and evolutionary implications of complete chloroplast genome
RT sequences of four early-diverging angiosperms: Buxus (Buxaceae),
RT Chloranthus (Chloranthaceae), Dioscorea (Dioscoreaceae), and Illicium
RT (Schisandraceae).";
RL Mol. Phylogenet. Evol. 45:547-563(2007).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC Rule:MF_01324}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR EMBL; EF380354; ABQ52509.1; -; Genomic_DNA.
DR RefSeq; YP_001294260.1; NC_009600.1.
DR AlphaFoldDB; A6MMT4; -.
DR GeneID; 5236773; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Metal-binding;
KW Nucleotidyltransferase; Plastid; Transcription; Transferase; Zinc.
FT CHAIN 1..1385
FT /note="DNA-directed RNA polymerase subunit beta''"
FT /id="PRO_0000353564"
FT BINDING 224
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 294
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 301
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 304
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ SEQUENCE 1385 AA; 157076 MW; 46844DF598EB07E4 CRC64;
MEVLMAERAN LVFHNKVIDG TAMKRLISRL IDHFGMAYTS HILDQVKTLG FQQATATSIS
LGIDDLLTIS SKGWLVQDAE QQSLLLEKHH RYGNVHAVEK LRQSIEIWYA TSEYLRQEMN
PNFRMTDPSN PVHIMSFSGA RGNVSQVHQL VGMRGLMSDP QGQMIDLPIQ SNLREGLSLT
EYTISCYGAR KGVVDTAVRT SDAGYLTRRL VEVVQHVVVR RTDCGTIRGI SISIRNGMTG
RILIQTLIGR VLADDVYMGL RCIAARNQDI GIGLVNRFIT SRAQPIYIRT PFTCRSTSWI
CRLCYGRSPT HGDLVELGEA VGIIAGQSIG EPGTQLTLRT FHTGGVFTGG TAEHVRAPFN
GKIKFNEDLV HPTRTRHGHP AFLCYIDLYV TIESQDILHN VNIPPKSFIL VQNDQYVESE
QVIAEIRART STFSFKEKVR KHIYSDSEGE IHWSTDVYHV PEYTHGNVHL LPKTTHLWIL
SGGPGPCRRS IVPFSLHKDQ DQMNLQSLSV EQRYISNLSV NNDRVRHKLF DLDPFWKKKG
GVGYSGPDRI LSNGHWDFIY PAILHENSNL LAKRRRNRFI ISFQHDQERE KKLPPRSGIK
IEIPINGILR RNSILAYFDD PRYRRSSSGI TKYGTIEVDS IVKKDDLIEY RGAKELRPKY
QMKVDPFFFI PEEVHILPGS SPIMVRNNSI IGVDTRITLN TRSRIGGLVR VERKKKRIEL
KIFSGDIHFP GETDKISRHS GILIPPETRK KNSKESKSKQ LKNWIYVQRI TPIKKKYFVS
VRAVVIYEIA DGIHLATLFP QDLLQERDNL QLRVVNYFLY GNGKPIRGIS HKSIQLVRTC
LVLNWDQDQN GSIEEVYASF VKVRANDLIR DFIRIDLVKS PISKRNDTAG SGLIPVNGSD
RTNINPFCSK ARIQSLSQHQ GTVRTFLNRN KEGESFLVLS SSNCSRIGPF NGLKYHNLTK
KESIQIKEDP MIPIRNSLGP FGTVPKIANF YSSYQLITHN EILLNKYLLL DNPKQTFEAL
KYYFMDENGI IYNPDPCSNI ILNPFDSNWY FPHQDYCEET CTIISLGQFI CENVCISKHG
PRIKSGQVLI VHVDSLVIRS AKPHLATPGA TVHGHYGEIL YEGNTLVTFI YEKSRSGDIT
QGLPKVEQVL EVRSIDSISM NLEKRFEGWG ERMTGILGIP WGFLIGAELT IAQSHFSLVN
KIQRVYRSQG VQIHNRHIEI IVRQITSKVL VSEDGMSNVF SPGELIGLLR AERTGRALEE
AICYRAVLLG ITRASLNTQS FISEASFQET TRVLAKAALR GRIDWLKGLK ENVVLGGMIP
VGTGFKGLVH HSREHNNIPF EIQKKSRFEG EMRDILFPHR ELFCSCLETN FHDTSEQSFM
GLNDS
