ID   RPOC2_IPOPU             Reviewed;        1401 AA.
AC   A7Y3B4;
DT   04-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT   23-OCT-2007, sequence version 1.
DT   03-AUG-2022, entry version 50.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE   AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE            Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN   Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324};
OS   Ipomoea purpurea (Common morning glory) (Pharbitis purpurea).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Convolvulaceae; Ipomoeeae; Ipomoea.
OX   NCBI_TaxID=4121;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=17956636; DOI=10.1186/1471-2229-7-57;
RA   McNeal J.R., Kuehl J.V., Boore J.L., dePamphilis C.W.;
RT   "Complete plastid genome sequences suggest strong selection for retention
RT   of photosynthetic genes in the parasitic plant genus Cuscuta.";
RL   BMC Plant Biol. 7:57-57(2007).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01324}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC       Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01324}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC       Rule:MF_01324}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR   EMBL; EU118126; ABV02338.1; -; Genomic_DNA.
DR   RefSeq; YP_001468298.1; NC_009808.1.
DR   AlphaFoldDB; A7Y3B4; -.
DR   GeneID; 5601317; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.132.30; -; 1.
DR   Gene3D; 1.10.274.100; -; 1.
DR   HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR   InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR   InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR   InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR   InterPro; IPR007083; RNA_pol_Rpb1_4.
DR   InterPro; IPR007081; RNA_pol_Rpb1_5.
DR   InterPro; IPR038120; Rpb1_funnel_sf.
DR   PANTHER; PTHR19376; PTHR19376; 1.
DR   Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR   Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR   TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE   3: Inferred from homology;
KW   Chloroplast; DNA-directed RNA polymerase; Metal-binding;
KW   Nucleotidyltransferase; Plastid; Transcription; Transferase; Zinc.
FT   CHAIN           1..1401
FT                   /note="DNA-directed RNA polymerase subunit beta''"
FT                   /id="PRO_0000353565"
FT   BINDING         224
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT   BINDING         295
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT   BINDING         302
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT   BINDING         305
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ   SEQUENCE   1401 AA;  158226 MW;  E2A643FC2B2F344A CRC64;
     MEVLMAERAN LVFHNKVIDG TAMKRLISRL INHFGMAYTS HILDQVKTLG FQQATATSIS
     LGIDDLLTIP SKGWLVQDAE QQSLILEKHH HYGNVHAVEK LRQSIEIWYA TSEYLRQEMN
     PNFRMTDPLN PVHIMSFSGA RGNASQVHQL VGMRGLMSDP QGQMIDLPIQ SNLREGLSLT
     EYIISCYGAR KGVVDTAVRT SDAGYLTRRL VEVVQHIVVR RADCGTVRGV SVSPQNGMMP
     ERILIQTLIG RVLADDIYIG PRCIATRNQN IGVGLVNRFI TFRAQPISIR TPFTCRSTSW
     ICRLCYGRSP THGDLVELGE AVGIIAGQSI GEPGTQLTLR TFHTGGVFTG GTAEHVRAPS
     NGKIRFHEDL VHPTRTRHGH PAFLCSIDLY VTIESEDILH NVNIPPKSFL LVQNDQYVES
     EQVIAEIRAG TSTLNLKEKV RKHIYSDSDG EMHWSTDVYH APEFTYGNVH LLSKTSHLWI
     LLGEPCRSSL VSLSIHRDQD QKSAHSLFVK RRSISNLSET NDRARQKIFS AHFSGQKEDR
     IADYSDLNRI ICTDHSNLVY PAILPILAEN SDFLSNLLSK RRRNQFIIPL QSIQERKKEL
     MPCSGISMKI PTNGIFCGNS IIAYFDDPRY RRMSSGSTKY GTLEMHSIVK KEDLIQYRGV
     REFRPKHQKK VDRFFFIPEE VHILPGSSSI SIMVRNNSII GVDTQITLNI RSRVGGLVRV
     EKKKKRIELK IFSGDIYFPG RADKISRHSG VLIPPGTRKT NEKESKKVKN WIYVQRITPS
     KKKFFVLVRP VVTYEIMDGI TLATLFPPDL LQERDNVQLR VVNYILYGNG KPIRGISDTS
     IQLVRTCLVL NWNQDKKSSS SEAARASVVE LRTNDLIRHF LRIDFVKSPI SYIGKRNDPW
     GLGLLADNGL DWTNINPYSK ARIQQTLNQN QGTIHTFLNR NKGCQSLSIL SSSNCSRMDP
     VNGAKYHNVI QESIQEDPLI PIRNSLGPLG TSLPIANFYS FDRLLTHNQI LVTNYLQLDN
     LKQPFQVLKL KYYLIAENGK IYNPNPRSKI LLNPLNLNWY FLHHNYCAET SKIMSLGQFI
     CENVFIAKKR PPLKSGQVIL VQVDSVVIRS AKPYLATPGA TVRGLYGETF YEGDTLVTFH
     YEKSRSGDIT QGLPKVEQVL EVRSVDSISM NLERRVEGWN KCLTRILGIP WGFLIGAELT
     IAQSRISLVN KIQKVYRSQG VQIHNRHIEI IVRQITSNVL VSEDGMSNVF SPGELIRLLR
     AKRMGRALEE AICYRALLLG ITKASLNTQS FISEASFQET ARVLAKAALQ GRIDWLKGLK
     ENVVLGGVIP AGTGFRGLVH PSKQYNNLSL ETTKKNLFEG GVRDILSHHR KLLDSSLSKK
     FHNTSHNTIH QTIIYRIYND S