ID   RPOC2_JASNU             Reviewed;        1397 AA.
AC   Q06RE1;
DT   06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   31-OCT-2006, sequence version 1.
DT   03-AUG-2022, entry version 50.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE   AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE            Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN   Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324}; ORFNames=JNC0181;
OS   Jasminum nudiflorum (Winter jasmine).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Lamiales; Oleaceae; Jasmineae; Jasminum.
OX   NCBI_TaxID=126431;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=17329229; DOI=10.1093/molbev/msm036;
RA   Lee H.-L., Jansen R.K., Chumley T.W., Kim K.-J.;
RT   "Gene relocations within chloroplast genomes of Jasminum and Menodora
RT   (Oleaceae) are due to multiple, overlapping inversions.";
RL   Mol. Biol. Evol. 24:1161-1180(2007).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01324}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC       Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01324}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC       Rule:MF_01324}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR   EMBL; DQ673255; ABG74618.1; -; Genomic_DNA.
DR   RefSeq; YP_778480.1; NC_008407.1.
DR   AlphaFoldDB; Q06RE1; -.
DR   GeneID; 4319742; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.132.30; -; 1.
DR   Gene3D; 1.10.274.100; -; 1.
DR   HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR   InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR   InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR   InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR   InterPro; IPR007083; RNA_pol_Rpb1_4.
DR   InterPro; IPR007081; RNA_pol_Rpb1_5.
DR   InterPro; IPR038120; Rpb1_funnel_sf.
DR   PANTHER; PTHR19376; PTHR19376; 1.
DR   Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR   Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR   TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE   3: Inferred from homology;
KW   Chloroplast; DNA-directed RNA polymerase; Metal-binding;
KW   Nucleotidyltransferase; Plastid; Transcription; Transferase; Zinc.
FT   CHAIN           1..1397
FT                   /note="DNA-directed RNA polymerase subunit beta''"
FT                   /id="PRO_0000277193"
FT   REGION          502..521
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         224
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT   BINDING         295
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT   BINDING         302
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT   BINDING         305
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ   SEQUENCE   1397 AA;  158717 MW;  4DB8AC55303B6005 CRC64;
     MEVLMAERAN LVFHNKVIDG TAMKRLINRL IEHFGMAYTS HILDQVKALG FHQATTTSIS
     LGIDDLLTIP SKRWLVQDAE QQSLVLEKHH HYGNVHAVEK LRQSVEIWYA TSEYLRQEMN
     PNFRMTDPFN PVHIMSFSGA RGNASQVHQL IGMRGLMSDP QGQMIDLPIQ SNLREGLSLT
     EYIISCYGAR KGVVDTAVRT SDAGYLTRRL VEVVQHIVVR RTDCGTIRGI SLSPRNGMMP
     ERVFIQTLMG RVLADDIYIG TRCLATRNQD IGIGLVNRFI TFRAQPISIR TPFTCRSASW
     ICRFCYGRSP THGDLVELGE AVGIIAGQSI GEPGTQLTLR TFHTGGVFTG GTAEHVRAPS
     NGKIKFNEDL VHPTRTRHGH PAFLCSIDLY VTIESEDILH NVNIPPKSFL LVQNDQYVES
     EQVIAEICVG TSTLNFKEKV RKHIFSDSDG EMHWSTDVYH APEFTYGNVH LLPKTSHLWI
     LLGGPCRSSR VSLSFHKDQD QMNAHSPSIK RRYTSNLSET NDPERQKLFS SYFYGKKKKK
     NRISDYSNLN QILCTGHCNF ISPTTLHQNS DLFSKRRKNK FLIPLQSIQE PENKLMPRSS
     ISIEIPQNGT FRGNSILAYF DDPRYRRKGS GIPQYGTIEM HSIVKKEDLV EYRGVKAFRP
     KYQMKVDRLF FIPEEVHILP RSSSIMVRNN SLIGVDTQLT LNIRSRVGGF VRVERKKKRI
     ELKIFSGDIH FPGETNKISR HSDVLIPPGK PNSKESKKWK NGIYVQRITP RKKKFFVLVR
     PVITYEITDG ININLATLFP PDLLQERDNV QLRVVNYILY ENGNPIRGFS HTDSQLVRTC
     LVLNWDQDKK KSASSEEARA SFVEIRTNGL IRHFLRIDLV KSPISYIGKR NDPSGSGLPS
     DNGSGWTNMN PFSSIYSKAR IQQSLNQNQG TIHTLLNRNT GFQSLRILSS SNCSRMGPFN
     DVIKYHKESI PITLITKDPF IPIKNFKNSL GPFGTAFPIA NFDSFSHLIT QNRILVNNYL
     ELDNFKQTFQ VIQYYLMDEN PKIYNPDLGS NILLNPFNWN WYFYHHTYCQ ETSPIISLGQ
     FICENVCIAK NAPHLKSGQV ILVQVASVVI RSAKPYLATP GTTVHGHYGE ILYEGDTLIT
     FLYEKSRSSD ITQGLPKVEE VFEVRSIDSI SMNLEKRFKG WNKRITRILG MPWGFLIGAE
     LTIVQSRISL VNKIQRVYRS QGVQIHNRHI EIIVRQITSK VLVSEDGMSN VFSPGELIGL
     LRAERMGRAL EEAVYYRAIL LGITRASLNT QSFISEASFQ ETARVLAKAA LRGRIDWLKG
     LKENVVLGGM IPLGTGFKGL VPPSKQDNNS SLETKKKNLF EGEMRDIFFH HRKLFDSCRS
     KNFHDTSEQS LLGFHDS