RPOC2_LACSA
ID RPOC2_LACSA Reviewed; 1381 AA.
AC Q56P11; Q1KXP1; Q332Y9;
DT 21-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 24-JAN-2006, sequence version 2.
DT 03-AUG-2022, entry version 63.
DE RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324}; ORFNames=PSC014;
OS Lactuca sativa (Garden lettuce).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; campanulids; Asterales; Asteraceae; Cichorioideae; Cichorieae;
OC Lactucinae; Lactuca.
OX NCBI_TaxID=4236;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=15917497; DOI=10.1093/molbev/msi174;
RA Kim K.-J., Choi K.-S., Jansen R.K.;
RT "Two chloroplast DNA inversions originated simultaneously during the early
RT evolution of the sunflower family (Asteraceae).";
RL Mol. Biol. Evol. 22:1783-1792(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Cisco;
RX PubMed=16604461; DOI=10.1007/s11248-005-3997-2;
RA Kanamoto H., Yamashita A., Asao H., Okumura S., Takase H., Hattori M.,
RA Yokota A., Tomizawa K.;
RT "Efficient and stable transformation of Lactuca sativa L. cv. Cisco
RT (lettuce) plastids.";
RL Transgenic Res. 15:205-217(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Salinas;
RA Timme R.E., Kuehl J.V., Boore J.L., Jansen R.K.;
RT "A comparison of the first two published chloroplast genomes in Asteraceae:
RT Lactuca and Helianthus.";
RL Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC Rule:MF_01324}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR EMBL; AY865171; AAX58144.1; -; Genomic_DNA.
DR EMBL; AP007232; BAE47583.1; -; Genomic_DNA.
DR EMBL; DQ383816; ABD47222.1; -; Genomic_DNA.
DR RefSeq; YP_398318.1; NC_007578.1.
DR AlphaFoldDB; Q56P11; -.
DR EnsemblPlants; rna-gnl|WGS:NBSK|LSAT_9X5941_mrna; cds-PLY79379.1; gene-LSAT_9X5941.
DR GeneID; 3772824; -.
DR Gramene; rna-gnl|WGS:NBSK|LSAT_9X5941_mrna; cds-PLY79379.1; gene-LSAT_9X5941.
DR KEGG; lsv:3772824; -.
DR OrthoDB; 731145at2759; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Metal-binding;
KW Nucleotidyltransferase; Plastid; Transcription; Transferase; Zinc.
FT CHAIN 1..1381
FT /note="DNA-directed RNA polymerase subunit beta''"
FT /id="PRO_0000067927"
FT BINDING 224
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 295
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 302
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 305
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT CONFLICT 1369..1381
FT /note="SNNFHDTQEQSFF -> QIISMIHKNNHFL (in Ref. 1;
FT AAX58144)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1381 AA; 156921 MW; EC55859E6B2659D8 CRC64;
MEVLMAERPT QVFHNKVIDG TAMKRLISRF IDHYGIGYTS HILDQVKTLG FRQATAASIS
LGIDDLLTIP SKRWLVQDAE QQSFILEKHH HYGNVHAVEK LRQSIEIWYA TSEYLRQEMN
PNFRMTDPFN PVHIMSFSGA RGNASQVHQL VGMRGLMSDP QGQMIDLPIQ SNLREGLSLT
EYIISCYGAR KGVVDTAIRT SDAGYLTRRL VEVVQHIVVR RTDCGTVRGI SVSPRNGMMT
DRIFIQTLIG RVLADDIYIG SRCIATRNQD IGVGLVSRFI TFRAQPISIR TPFTCRSTSW
ICQLCYGRSP AHDDLVELGE AVGIIAGQSI GEPGTQLTLR TFHTGGVFTG GTAEHVRAPS
NGKIKFNEDL VHPTRTRHGH PAFLCSRDLY VTIESEDIIH NVCIPPKSFL LVQNDQYVES
EQVIAEIRAR TSTLNLKEKV RKHIYSDSEG EMHWNTDVYH APEFTYGNIH LLPKTSHLWI
LLGEPWRYSL GPCSIHKDQD QMNAYSLSVK PRYIANPSVT NNQVRHKFFS SYFSGKNQKG
DRIPDCSELN RMTCTDHSNL RYPAILDGNS DLLAKRRRNR FIIPLESIQE GENQLIPSSG
ISMEIPRNGI LRRNSILAYF DDPRYIRKSS GLTKYETREL NSIVNEENLI EYRGVKVFWP
KYQKEVNPFF FIPVEVHILS ESSSIMVRHN SIIGADTQIT FNRRSRVGGL VRVKKKAEKM
KLIIFSGDIH FPGKTNKAFR LIPPGGGKPN SKEYKKLKNW LYIQRMKLSR YEKKYFVLVQ
PVVPYKKTDG INLGRLFPPD LLQESDNLQL RVVNYILYYD PILEIWDTSI QLVRTSLVLN
WDQDKKIEKA CASFVEIRTN GLLRYFLRID LAKSPISYTG KRNDLSGSGL ISENGSDRAN
VNPFSSIYSY SKSRIKESLN PNQGTIHTLL NRNKESQSLI ILSSSNCFRI GPFNDVKSPN
VIKESIKKNP LIPIRNSLGP LGTGFPIYNF DLFSHLITHN QILVTNYLQL DNFKQIFQIL
KYYLLDENGQ IYNPYSCSNI ILNPFHLNWY FLHYNYCEET SPIVSLGQFL CENVCIAKKG
PHLKSGQVLI VQVDSVVIRS AKPYLATPGA TVHGHYGEIL YEGDTLVTFI YEKSRSGDIT
QGLPKVEQVL EVRSIDSISM NLEKRIEGWN KSITRILGIP WAFLIGAELT IVQSRISLVN
KVQKVYRSQG VQIHNRHIEI IVRQITSKVL VSEDEMSNVF SPGELIGLLR AERMGRALEE
AICYQAVLLG ITRASMNTQS FISEASFQET ARVLAKAALL GRIDWLKGLK ENVVLGGMIP
VGSGFKTPSS EPNNIPNNIA FELQKKNLLE GEMKDILFYH RKLFDSCLSN NFHDTQEQSF
F
