RPOC2_LEMMI
ID RPOC2_LEMMI Reviewed; 1366 AA.
AC A9L986;
DT 04-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT 05-FEB-2008, sequence version 1.
DT 03-AUG-2022, entry version 49.
DE RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324};
OS Lemna minor (Common duckweed).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Araceae; Lemnoideae; Lemna.
OX NCBI_TaxID=4472;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=18463914; DOI=10.1007/s00239-008-9091-7;
RA Mardanov A.V., Ravin N.V., Kuznetsov B.B., Samigullin T.H., Antonov A.S.,
RA Kolganova T.V., Skyabin K.G.;
RT "Complete sequence of the Duckweed (Lemna minor) chloroplast genome:
RT structural organization and phylogenetic relationships to other
RT angiosperms.";
RL J. Mol. Evol. 66:555-564(2008).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC Rule:MF_01324}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR EMBL; DQ400350; ABD48485.1; -; Genomic_DNA.
DR RefSeq; YP_001595498.1; NC_010109.1.
DR AlphaFoldDB; A9L986; -.
DR GeneID; 5787539; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Metal-binding;
KW Nucleotidyltransferase; Plastid; Transcription; Transferase; Zinc.
FT CHAIN 1..1366
FT /note="DNA-directed RNA polymerase subunit beta''"
FT /id="PRO_0000353566"
FT BINDING 220
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 290
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 297
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 300
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ SEQUENCE 1366 AA; 154138 MW; 10712571B1DB2EF3 CRC64;
MAERADLVFH NKVIDGAAMK RLISRLIDHF GMAYTSHILD QVKTLGFQQA TATSISLGID
DLLTIPSKGW LVQDAEQQSL ILEKHHHYGN VHAVEKLRQS IEIWYATSEY LRQEMNPNFR
MTDPSNPVHL MSFSGARGNA SQVHQLVGMR GLMSDPQGQM IDLPIQSNLR EGLSLTEYII
SCYGARKGVV DTAVRTSDAG YLTRRLVEVV QHIIVRRTDC GTIRSISVSP RNGVPERIFI
QTLIGRVLAD DIYIGPRCIA ARNQDIGIGL VNRFISFRAQ PIHIRTPFTC RSTSWICQLC
YGRSPTHGDL VELGEAVGII AGQSIGEPGT QLTLRTFHTG GVFTGGTAEH VRAPFNGKIK
FNEDLVHPTR TRHGHPAFLC SIDLYVTIES QDIIHNVNIP PKSLILVQND QYVESEQVIA
EIRAGAATLN FKEKVRKHIY SESEGEMHWS TDVYHAPEYT YGNVHLLPKT SHLWILAVGT
CRYSIVSFSL HKDQDQINAH SLSLEERYIS DLSVTKDLAR HQLFSSDSSS KKRGGTFDYA
RPDGSISNGH WNLQYPFILH ENSDFLAKRR RNKFIIPLQY DQERDKELIP HFGVSIEIPI
NGILRRNSIL AYFDDPRYRR NSSGITKYGA VEVDSILKKE DLIEYRGTKE LSPKYQTKVD
RFFFIPEEVH VLPGSSLIMV RNNSIIGVDT PLTLNIRSRV GGLVRVERKK KSIELKIFSG
DIHFPGETDK ISRHSGILIP PGTGKMTSKE SKKLKNWIYV QRITPTKKKY FVSVRPVVTY
EIADGINLAT FFPKDLLQEG DNIQLRVVNY ILYGNGKPIR GISHTSIQLV RTCLVLNWHQ
EEKSSIEDSH ASFVEIKTND LTCNFIRIEL GKSPLLYTGK RNSVVSSGLI NHNGLDYLNS
NPFYSKAKIP SLTKQKGTGG TLLNGNKECQ SLKILLSSNY SRIGPFNGLK YNTVTKQSIK
ADPVTPIRNC LGLLGTVVLK IANFYSSCHL ITYNKTLLKK YFLIENLSQT FQGIIFFLID
ENRGIYNLDP CSKTVFNPFD LNWRFFHHDY CDETSTLITL GQIFCENVCL FKYGSQKKSG
QVIIVHVDYL VIRSAKPYLA TPGATVHGHY GETLHEGDTL VTFIYEKSRS GDITQGLPKV
EQVLEVRSID SISTNLEKRV KAWNERISRI LGNPWGFLIS TELTIAQSRI SLVNKIQKVY
RSQGVQIHNR HLEIIVRQVT SKVLVSEDGM SNVFLPGELI GLLRAERAGR ALDEAISYRA
ILLGITRASL NTQSFISEAS FQETARVLAK AALRGRIDWL KGLKENVVLG GIIPVGTGFK
KLVHHSRQHK NIHLEIKNKN LFEGKMRDIL FHHREFLSSC IPNNFQ
