RPOC2_LIRTU
ID RPOC2_LIRTU Reviewed; 1382 AA.
AC Q0G9M9;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 51.
DE RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324};
OS Liriodendron tulipifera (Tuliptree) (Tulip poplar).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Magnoliidae; Magnoliales; Magnoliaceae;
OC Liriodendron.
OX NCBI_TaxID=3415;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=17020608; DOI=10.1186/1471-2148-6-77;
RA Cai Z., Penaflor C., Kuehl J.V., Leebens-Mack J., Carlson J.E.,
RA dePamphilis C.W., Boore J.L., Jansen R.K.;
RT "Complete plastid genome sequences of Drimys, Liriodendron, and Piper:
RT implications for the phylogenetic relationships of magnoliids.";
RL BMC Evol. Biol. 6:77-77(2006).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC Rule:MF_01324}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR EMBL; DQ899947; ABI32499.1; -; Genomic_DNA.
DR RefSeq; YP_740192.1; NC_008326.1.
DR AlphaFoldDB; Q0G9M9; -.
DR GeneID; 4266600; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Metal-binding;
KW Nucleotidyltransferase; Plastid; Transcription; Transferase; Zinc.
FT CHAIN 1..1382
FT /note="DNA-directed RNA polymerase subunit beta''"
FT /id="PRO_0000277194"
FT BINDING 224
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 294
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 301
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 304
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ SEQUENCE 1382 AA; 156911 MW; 9A3F4B79DAC2B62E CRC64;
MEVLMAERAD LVFHNKVIDA TAMKRLISRL IDHFGMAYTS HILDQVKTLG FQQATATSIS
LGIDDLLTIP SKGWLVQDAE QQSLILEKHH HYGNVHAVEK LRQSIEIWYA TSEYLRQEMH
PNFRMTDPSN PVHIMSFSGA RGNASQVHQL VGMRGLMSDP QGQMIDLPIQ SNLREGLSLT
EYIISCYGAR KGVVDTAVRT SDAGYLTRRL VEVVQHIVVR RTDCGTIRGI SVSPRNGMTE
KILIQTLIGR VLADDIYMGL RCIATRNQDI GIGLVNRFIT FRAQSIYIRT PFICRSTSWI
CRLCYGRSPT HGDLVELGEA VGIIAGQSIG EPGTQLTLRT FHTGGVFTGG TAEHVRAPSN
GKIKFNECLV HPTRTRHGHP AFLCYIDLYV TIESQDIIHN VNIPPKSFLL VQNDQYVESE
QVIAEIRAGT STFNFKERVR KHIYSDSEGE MHWSTDVYHA PEYRYGNVHL LPKTSHLWIL
SGGPCRSSIV PFSLHKDQDQ MNVHSLSVER RYISDLSVTN DRVRHKLFSS DPSGKKKERI
LDYSGPDRIV SNGHWNFLYP AILHENSDLL AKRRRNRFII PFQYDQEREK ELMPRSGISI
EIPINGILRR DTILAYFDDP RYRRSSSGIT KYGTIEVDSI VKKEDLIEYR GAKEFRPKYQ
MKVDRFFFIP EEVHILPGSS PIMVRNNSII GVDTRIALNT RSRVGGLVRV ERKKKKIELK
IFSGDIHFPG ETDKISRHSG ILIPPGTGKK NSKESKKWKN WIYVQRITPT KKKYFVSVRP
VVTYEIADGI NLGTLFPQDL LQERDNVQLR VVNYILYGNG KPIRGIYHTS IQLVRTCLVL
NWDQDRNGSI EEVHASFVEV GTNDLIRDFI RIDLVKSPIS YIGKRDDTTG SGLIPDNESD
RTNINTFYSK TRIQSLTQHQ GTIRTFLNRN KECQSFLILS SSDCSRIGPF NGSKSHKVTK
ESIKEDPMIP IRNSLGPLGT VPKIANFYSS YYLITHNQIL LNKYLLLDNL KQTFQVLKYY
LMDENGRIYN PNLHSNIIFN PFDLNWCFLR HDYCEETSTI ISLGQFICEN VCISKYGPHI
KSGQVLIVHV DSLVIRSAKP HLATPGATVH GHYGEILSEG DTLVTFIYEK SRSGDITQGL
PKVEQVLEVR SIDSISMNLE KRIEGWNEHI TRILGIPWGF LIGAELTIAQ SRISLVNKIQ
KVYRSQGVQI HNRHIEIIVR QITSKVLVSE DGMSNVFSPG ELIGLLRAER TGRALEEGIC
YRAILLGITR ASLNTQSFIS EASFQETARV LAKAALRGRI DWLKGLKENV VLGGMIPVGT
GFKGLVHRSR QHNNIPLEIK KKNLFEGEMR DILFHHRELL SSCIPKNFHD TSEQSFTGFN
DS
