RPOC2_LOTJA
ID RPOC2_LOTJA Reviewed; 1332 AA.
AC Q9BBS7;
DT 10-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 78.
DE RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324};
OS Lotus japonicus (Lotus corniculatus var. japonicus).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; robinioid clade; Loteae; Lotus.
OX NCBI_TaxID=34305;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Miyakojima MG-20;
RX PubMed=11214967; DOI=10.1093/dnares/7.6.323;
RA Kato T., Kaneko T., Sato S., Nakamura Y., Tabata S.;
RT "Complete structure of the chloroplast genome of a legume, Lotus
RT japonicus.";
RL DNA Res. 7:323-330(2000).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC Rule:MF_01324}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR EMBL; AP002983; BAB33196.1; -; Genomic_DNA.
DR RefSeq; NP_084798.1; NC_002694.1.
DR AlphaFoldDB; Q9BBS7; -.
DR GeneID; 802880; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Metal-binding;
KW Nucleotidyltransferase; Plastid; Transcription; Transferase; Zinc.
FT CHAIN 1..1332
FT /note="DNA-directed RNA polymerase subunit beta''"
FT /id="PRO_0000067928"
FT BINDING 220
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 291
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 298
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 301
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ SEQUENCE 1332 AA; 151853 MW; BDA32FF920B2B8C1 CRC64;
MAERANLVFH NKVIGGTAIK RIISRLIDHF GMAYTSHILD QVKTLGFRQA TTTSISLGID
DLLTIPSKGW LVQDAEQQSL ILEKHHHYGN VHAVEKLRQS IEIWYATSEY LRQEMNPNFS
MTDPFNPVHI MSFSGARGNA SQVHQLVGMR GLMSDPQGQM IDLPIQSNLR EGLSLTEYII
SCYGARKGVV DTAVRTSDAG YLTRRLVEVV QHIVVRRTDC GTVRGISVNT RNRMMSERIL
IQTLIGRVLA DDIYIGSRCI VVRNQDIGIG LINRFINFQT QPIFIRTPFT CRNTSWICRL
CYGRSPIHGN LVELGEAVGI IAGQSIGEPG TQLTLRTFHT GGVFTGGTAE YVRSPSNGKI
KFNENSAYPT RTRHGHPAFL CYIDLYVTIE SNDIMHNVII PPKSFLLVQN DQYVKSEQII
AEIRAGTYTL NLKEKVRKHI FSDSEGEMHW STNIYHVSEF AYSNVHILPK TSHLWILSGN
SHKSDTVSLS LLKDQDQMST HSLPTAKRNT SNFLVSNNQV RLCPDHCHFM HPTISPDTSN
LLAKKRRNRF IIPFLFRSIR ERNNELMPDI SVEIPIDGII HKNSILAYFD DPQYRTQSSG
IAKYKTIGIH SIFQKEDLIE YRGIREFKPK YQIKVDRFFF IPQEVHILSE SSSIMVRNNS
IIGVNTPITL NKKSRVGGLV RVEKNKKKIE LKIFSGDIHF PGEIDKISQH SAILIPPEMV
KKKNSKESKK KTNWRYIQWI TTTKKKYFVL VRPVILYDIA DSINLVKLFP QDLFKEWDNL
ELKVLNFILY GNGKSIRGIL DTSIQLVRTC LVLNWNEDEK SSSIEEALAS FVEVSTNGLI
RYFLRIDLVK SHISYIRKRN DPSSSGLISY NESDRININP FFSIYKENIQ QSLSQKHGTI
RMLLNRNKEN RSFIILSSSN CFQMGPFNNV KYHNGIKEEI NQFKRNHKIP IKISLGPLGV
APQIANFFSF YHLITHNKIS SIKKNLQLNK FKETFQVIKY YLMDENERIY KPDLYNNIIL
NPFHLNWDFI HPNYCEKTFP IISLGQFICE NVCIVQTKNG PNLKSGQVIT VQMDFVGIRL
ANPYLATPGA TIHGHYGEML YEGDILVTFI YEKSRSGDIT QGLPKVEQVL EVRSIDSISM
NLEKRIDAWN ERITGILGIP WRFLIGAELT IAQSRISLVN KIQRVYRSQG VHIHNRHIEI
IVRQITSKVL VSEDGMSNVF SPGELIGLLR AQRTGRALEE SICYRTLLLG ITKTSLNTQS
FISEASFQET ARVLAKAALR GRIDWLKGLK ENLVLGGIIP VGTGFKKIGD RSSARQDTKI
TLETIKIIRG RN
