RPOC2_MESVI
ID RPOC2_MESVI Reviewed; 1223 AA.
AC Q9MUS7;
DT 27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 85.
DE RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324};
OS Mesostigma viride (Green alga).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Mesostigmatophyceae;
OC Mesostigmatales; Mesostigmataceae; Mesostigma.
OX NCBI_TaxID=41882;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NIES-296 / KY-14 / CCMP 2046;
RX PubMed=10688199; DOI=10.1038/35001059;
RA Lemieux C., Otis C., Turmel M.;
RT "Ancestral chloroplast genome in Mesostigma viride reveals an early branch
RT of green plant evolution.";
RL Nature 403:649-652(2000).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC Rule:MF_01324}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR EMBL; AF166114; AAF43824.1; -; Genomic_DNA.
DR RefSeq; NP_038383.1; NC_002186.1.
DR AlphaFoldDB; Q9MUS7; -.
DR SMR; Q9MUS7; -.
DR GeneID; 800897; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 4.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Metal-binding;
KW Nucleotidyltransferase; Plastid; Transcription; Transferase; Zinc.
FT CHAIN 1..1223
FT /note="DNA-directed RNA polymerase subunit beta''"
FT /id="PRO_0000067931"
FT BINDING 233
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 307
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 314
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 317
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ SEQUENCE 1223 AA; 138049 MW; 4044E90805FC472A CRC64;
MGRMKKIFFN QKSDLNSMKL FFCNRIMNKG EIRRIIAWFL FNHGTSRTAH MVDRLKILGF
YHATKAGISL SIDDLSIPPT KKWLLEDAEQ EIAKTQKNYS IGKITAVERF QKVIDTWHST
SETLKNEVVQ YFEQTNPLNP VYMMAFSGAR GNLSQVSQLV GMRGLMSDPQ GQIIDLPIRS
NFREGLTVTE YVISCYGARK GLVDTALRTA DSGYLTRRLV DVAQDMIIRE TDCGTKSGIL
LGPVKDEQKV VFSLQERLIG RVLAENIYSP KQKQYIAKKN QDISASLSDK ICEIKRTNIL
VRSPLTCKSI RSVCQLCYGW SLAHGNLVDL GEAVGIIAAQ SIGEPGTQLT MRTFHTGGVF
TGNIAKQIRS SIDGKIIYSL TNTIPMRTRH GEIALITQSN INICIQGKNK EFNIEVPINT
ILLVNNGSMV RAKQLIAEVS APVNAQSVEI ASKYVASDFS GEIHFENLLL QEKKSAYGHK
MNRAGQYGGL LWILSGQVYK FSLDVPLMFD TADYIHNGSC MAEHNIISQY GGILQTSENN
LTIDNSNSYL YVLNSSMTII NAYLSHIKNA RSEAYILTDD LNQKFFMQVE SNNVIKNNQI
IAKFVTKTYL TNTGGILKYS SEIEVDEYDI VTQSYPVRKG GIIMWIPEET HLINKDASLL
MVRDCEYVEA GTQIIKDLIC HSSGLAQVSQ TNDILREVVI KPGKIYRPID FSQVLDKHQT
LIQAGEEICD GIIAEELVYL ENVNLSSGSA LLIRPVVQFL IPNSTELKKL EAQYQLKQNI
SIQPSIHICY KDGQRIHAWQ SMEIAKIFLR LEIDSHLHKL NVTLNLIENK LLQIKMFEYL
MIDLNQIIDF QSYQNVTRLV RKFNNYIIPG TILAKTEIIA KDHGEVRQSN KNFEEQKSFL
IMNEHDQMTL FVKNAYEFFQ LGDLVHKGKE IVPGILAPQS GQVIQLQANR VTLRLARPYL
VSSEAVLYVD DGDFVKSGDT LVMLIFEQSK TGDIVQGLPR IEELLEARRT KGLKPLPNNL
HDLLQSFFDE ATEKYGIQKA AKKSFEKLQL FLVNEVQSVY KAQGVHISDK HIEVIVRQMT
SKVMIEEGGD TTLLPGELIE LHRIENMNRN VSVHAKYKPI VLGITKASLN TESFISAASF
QETTRVLTKA AIEGKTDWLR GLKENVIIGR LIPAGTGFNV HDKMYSRDLS LSNSYNLAYS
SNSQHKRIEQ KNAEYNFEDI IFD
