RPOC2_NANDO
ID RPOC2_NANDO Reviewed; 1377 AA.
AC Q09FX1;
DT 04-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT 17-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 55.
DE RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324};
OS Nandina domestica (Heavenly bamboo).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Ranunculales; Berberidaceae; Nandinoideae;
OC Nandina.
OX NCBI_TaxID=41776;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16934154; DOI=10.1186/1471-2229-6-17;
RA Moore M.J., Dhingra A., Soltis P.S., Shaw R., Farmerie W.G., Folta K.M.,
RA Soltis D.E.;
RT "Rapid and accurate pyrosequencing of angiosperm plastid genomes.";
RL BMC Plant Biol. 6:17-17(2006).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC Rule:MF_01324}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR EMBL; DQ923117; ABI49853.1; -; Genomic_DNA.
DR RefSeq; YP_740640.1; NC_008336.1.
DR AlphaFoldDB; Q09FX1; -.
DR GeneID; 4271570; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Metal-binding;
KW Nucleotidyltransferase; Plastid; Transcription; Transferase; Zinc.
FT CHAIN 1..1377
FT /note="DNA-directed RNA polymerase subunit beta''"
FT /id="PRO_0000353571"
FT BINDING 220
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 291
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 298
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 301
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ SEQUENCE 1377 AA; 156205 MW; 049B4BF15FC7587A CRC64;
MAERADLVFH NKAIDGTAMK RLISRLIDHF GMAYTSHILD QIKTLGFQQA TATSISLGID
DLLTIPSKGW LVQDAEQQSL ILEKHHHYGN VHAVEKLRQS IEIWYATSEY LRQEMNPNFR
MTDPSNPVHI MSFSGARGNA SQVHQLVGMR GLMSDPQGQM IDLPIQSNLR EGLSLTEYII
SCYGARKGVV DTAVRTSDAG YLTRRLVEVV QHIVVRRTDC GTLRGSSVSP RNGTITERIF
IQTLIGRVLA DDIYIGSRCI AIRNQDIGIG LVNRFITFRA QPISIRTPFT CKSTSWICRL
CYGRSPTHGD LVELGEAVGI IAGQSIGEPG TQLTLRTFHT GGVFTGGTAE HVRAPSNGKI
KFKEDLVHPT RTRHGHPAFL CYIDLYVTIE GQDIRHNVTI PPKSFLLVQN DQYVESEQVI
AEIRAGTSTL NFKERVRKHI YSDSEGEMHW STAVYHAPEY TYGNVHLLPK TSHLWILSGG
PYRSSIVSFS LHKDQDQMNV HSLSAEQKSI SNLLVTNNQV RHKFLSSEPS GKKGERILDY
SGPNRIICNG HCNFIYPAIL HENSYLLAKR RRNRFIIPFQ YNQEQEKELI TRSGISIEIP
INGIFRRNSI LAYFDDPRYR RNSSGITKYG TIEAHSIVKK EDLIEYRKRP KYQTKIDRFF
FIPEEVHILP GSSSVMVRNN SIIGVNTRIT FKTRSRVGGL VRVEKKKKKI ELKIFSGDIH
FPGETDKISW HSGILIPPGT GKKNSKESKN FKNWIYVQRI TPTKKKYFVL VRPVVTYEIA
DGINLATLFP QDPLQERDNV QLRVVNYILY GNGKPIRGIS HTNIQLVRTC LVLNWDQEKN
GSSMEEVHAS FVEVRVNNLI RDFIRMDLVK SPISYTRKRN APAGSELILA NGSDYTNLNP
FYSKARIQQS LTQHQGTIRT LLNRNKECQS LIFLSSSNCF RIGPFKGLKY NNMTKKSNKR
DSLLLIRNSL GPLGIVPKIA NFSSFYYLIT HNQILINKYL LLDNLKQTFQ KLKYYLMDEN
GRIYNPDPCS NMILNPFNWN WYFLHHDYCE ETSTIISLGQ FFCENVCISK YGPHLKSGQV
IIVHVDSLVI RSAKPYLATP GATVHGHYGE ILYKGDTLVT FIYEKSRSGD ITQGLPKVEQ
VLEVRSIDSI SMNLEKRVEG WNERITRILG IPWGFLIGAE LTIAQSRVSL VNKIQKVYRS
QGVQIHNRHI EIIVRQITSK VLVSEDGMSN VFSPGELIGL LRAERTGRAL EEAICYRAVL
LGITRASLNT QSFISEASFQ ETARVLAKAA LRGRIDWLKG LKENVVLGGM IPVGTGFKGL
VHRSSQHSNI SLEIQNTNLF EGGMRDILFH HRELFYSCIP KNFHDTSEQK FTGFIDS
