ID   RPOC2_NICTO             Reviewed;        1392 AA.
AC   Q33C48;
DT   07-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT   06-DEC-2005, sequence version 1.
DT   03-AUG-2022, entry version 54.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE   AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE            Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN   Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324};
OS   Nicotiana tomentosiformis (Tobacco).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4098;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16435119; DOI=10.1007/s00438-005-0092-6;
RA   Yukawa M., Tsudzuki T., Sugiura M.;
RT   "The chloroplast genome of Nicotiana sylvestris and Nicotiana
RT   tomentosiformis: complete sequencing confirms that the Nicotiana sylvestris
RT   progenitor is the maternal genome donor of Nicotiana tabacum.";
RL   Mol. Genet. Genomics 275:367-373(2006).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01324}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC       Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01324}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC       Rule:MF_01324}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR   EMBL; AB240139; BAE47987.1; -; Genomic_DNA.
DR   RefSeq; YP_398849.2; NC_007602.1.
DR   AlphaFoldDB; Q33C48; -.
DR   GeneID; 3776380; -.
DR   KEGG; nto:3776380; -.
DR   OrthoDB; 731145at2759; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.132.30; -; 1.
DR   Gene3D; 1.10.274.100; -; 1.
DR   HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR   InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR   InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR   InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR   InterPro; IPR007083; RNA_pol_Rpb1_4.
DR   InterPro; IPR007081; RNA_pol_Rpb1_5.
DR   InterPro; IPR038120; Rpb1_funnel_sf.
DR   PANTHER; PTHR19376; PTHR19376; 1.
DR   Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR   Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR   TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE   3: Inferred from homology;
KW   Chloroplast; DNA-directed RNA polymerase; Metal-binding;
KW   Nucleotidyltransferase; Plastid; Transcription; Transferase; Zinc.
FT   CHAIN           1..1392
FT                   /note="DNA-directed RNA polymerase subunit beta''"
FT                   /id="PRO_0000225335"
FT   BINDING         224
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT   BINDING         295
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT   BINDING         302
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT   BINDING         305
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ   SEQUENCE   1392 AA;  157231 MW;  3B6CABE4357878E9 CRC64;
     MEVLMAERAN LVFHNKAIDG TAMKRLISRL IDHFGMAYTS HILDQVKTLG FQQATATSIS
     LGIDDLLTIP SKGWLVQDAE QQSLILEKHH HYGNVHAVEK LRQSIEIWYA TSEYLRQEMN
     PNFRMTDPFN PVHIMSFSGA RGNASQVHQL VGMRGLMSDP QGQMIDLPIQ SNLREGLSLT
     EYIISCYGAR KGVVDTAVRT SDAGYLTRRL VEVVQHIVVR RTDCGTARGI SVSPRNGMMP
     ERFFIQTLIG RVLADDIYMG PRCIATRNQD IGIGLVNRFI TFRAQPISIR TPFTCRSTSW
     ICRLCYGRSP THGDLVELGE AVGIIAGQSI GEPGTQLTLR TFHTGGVFTG GTAEHVRAPS
     NGKIKFNEDL VHPTRTRHGH PAFLCSIDLY VTIESEDILH NVNIPPKSLL LVQNDQYVES
     EQVIAEIRAG ISTLNFKEKV RKHIYSDSDG EMHWSTDVYH APEFTYGNVH LLPKTSHLWI
     LLGGPCRSSL VYLSIHKDQD QMNAHFLSGK RRYTSNLSVT NDQARQKLFS SDFSVKKEDR
     IPDYSDLNRI ICAGQYNLVY SPILHENSDL LSKRRRNKFI IPLHSIQELE NELMPCSGIS
     IEIPVNGIFR RNSILAYFDD PRYRRKSSGI IKYGTVETHS VIKKEDLLEY RGVKEFSPKY
     QMKVDLFFFI PEEVHILPGS SSIMVRNNSI VGVDTQITLN LRSRVGGLVR VERKKKQIEL
     KIFSGDIHFP GETDKISRHT GVLIPPGTGK RNSKESKKVK NWIYVQRITP SKKRFFVLVR
     PVVTYEITDG INLATLFPPD PLQERDNVQL RIVNYILYGN GKPIRGISDT SIQLVRTCLV
     LNWNQDKKSS SCEEARASFV EIRTNGLIRH FLRINLVKSP ISYIGKRNDP SGSGLLSDNG
     SDCTNINPFS SIYSYSKAKI QQSLNQPQGT IHTLLNRNKE CQSLIILSAA NCSRMGPFKD
     VKYHSVIKES IKKDPLIPIR NSLGPLGTSL PIENFYSSYH LITHNQILVT NYLQLDNLKQ
     TFQVIKFHYY LMDENGKIFN PDPCRNIILN PFNLNWYFLH HNYCEETSKI ISLGQFICEN
     VCIAKNGPPL KSGQVILVQV DSIVIRSAKP YLATPGATVH GHYGETLYEG DTLVTFIYEK
     SRSGDITQGL PKVEQVLEVR SIDSISMNLE KRIEGWNKCI TRILGIPWGF LIGAELTIAQ
     SRISLVNKIQ QVYRSQGVQI HNRHIEIIVR QITSKVLVSE DGMSNVFSPG ELIGLLRAER
     MGRALEEAIC YRVVLLGITR ASLNTQSFIS EASFQETARV LAKAALRGRI DWLKGLKENV
     VLGGVIPVGT GFKGLVHPSK QHNNIPLETK KKNLFEGEMR DILFHHRKLF DSCLSKNFHD
     IPEQSFIGFN DS