RPOC2_NOSS1
ID RPOC2_NOSS1 Reviewed; 1355 AA.
AC P22705;
DT 01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2002, sequence version 2.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=DNA-directed RNA polymerase subunit beta' {ECO:0000255|HAMAP-Rule:MF_01324};
DE Short=RNAP subunit beta' {ECO:0000255|HAMAP-Rule:MF_01324};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=RNA polymerase subunit beta' {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=Transcriptase subunit beta' {ECO:0000255|HAMAP-Rule:MF_01324};
GN Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324}; OrderedLocusNames=alr1596;
OS Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576).
OC Bacteria; Cyanobacteria; Nostocales; Nostocaceae; Nostoc.
OX NCBI_TaxID=103690;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PCC 7120 / SAG 25.82 / UTEX 2576;
RX PubMed=11759840; DOI=10.1093/dnares/8.5.205;
RA Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A.,
RA Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M.,
RA Takazawa M., Yamada M., Yasuda M., Tabata S.;
RT "Complete genomic sequence of the filamentous nitrogen-fixing
RT cyanobacterium Anabaena sp. strain PCC 7120.";
RL DNA Res. 8:205-213(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-63.
RX PubMed=1904436; DOI=10.1128/jb.173.11.3446-3455.1991;
RA Bergsland K.J., Haselkorn R.;
RT "Evolutionary relationships among eubacteria, cyanobacteria, and
RT chloroplasts: evidence from the rpoC1 gene of Anabaena sp. strain PCC
RT 7120.";
RL J. Bacteriol. 173:3446-3455(1991).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- SUBUNIT: In cyanobacteria the RNAP catalytic core is composed of 2
CC alpha, 1 beta, 1 beta', 1 gamma and 1 omega subunit. When a sigma
CC factor is associated with the core the holoenzyme is formed, which can
CC initiate transcription. {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB77962.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; BA000019; BAB77962.1; ALT_INIT; Genomic_DNA.
DR EMBL; M60831; AAA22034.1; -; Genomic_DNA.
DR PIR; AF2005; AF2005.
DR PIR; C42361; C42361.
DR RefSeq; WP_044521003.1; NZ_RSCN01000041.1.
DR AlphaFoldDB; P22705; -.
DR SMR; P22705; -.
DR STRING; 103690.17135416; -.
DR EnsemblBacteria; BAB77962; BAB77962; BAB77962.
DR KEGG; ana:alr1596; -.
DR eggNOG; COG0086; Bacteria.
DR OMA; IEGKSDW; -.
DR OrthoDB; 4373at2; -.
DR Proteomes; UP000002483; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR007066; RNA_pol_Rpb1_3.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 4.
DR Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Metal-binding; Nucleotidyltransferase;
KW Reference proteome; Transcription; Transferase; Zinc.
FT CHAIN 1..1355
FT /note="DNA-directed RNA polymerase subunit beta'"
FT /id="PRO_0000067902"
FT REGION 1331..1355
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 219
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 293
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 300
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 303
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ SEQUENCE 1355 AA; 147583 MW; 761EE9D92870C961 CRC64;
MTNEKMIFRN RVVDKGQLRN LISWAFTHYG TARTAVMADK LKDLGFRYAT RAGVSISVDD
LMVPPSKRSL LEAAEEEIRA TEVRYQRGEI TEVERFQKVI DTWNGTSEAL KDEVVTHFKQ
TNPLNSVYMM AFSGARGNIS QVRQLVGMRG LMADPQGEII DLPIKTNFRE GLTVTEYIIS
SYGARKGLVD TALRTADSGY LTRRLVDVSQ DVIIREIDCG TTRGIPVRPM TEGSKTLIKL
STRLLGRVVG EDVIHPKTKE VIAPRNTPIS DDLAKEIEKA GVAEVVVRSP LTCEAARSVC
QHCYGWSLAH AKMVDLGEAV GIIAAQSIGE PGTQLTMRTF HTGGVFTGEV AQQVRSKMDG
TIKLPRKLRT RTHRTRHGED ALFVESNGIM ILEPRKEGSE TPAPQEIHVT QGSTIYIVDG
QQVKKGQLLA EVALGGRTTR TNTEKAVKDV ASDLAGEVKF AEVVPEQKTD RQGNTTTTAA
RGGLIWILSG EVYNLPPGAE LVVKNGDRVE TNGVLAETKL TTIHGGVVRL PEATPGKSTR
EIEIITASVV LDQATVTVQS SQGRNNYLIT TGNNQVFNLR ATPGTKVQNG QVVAELIDDR
YRTTTGGFLK FAGVEVQKKG KAKLGYEVVQ GGTLLWIPEE THEVNKDISL LLVEDGQYVE
AGTEVVKDIF CQNSGVVEVT QKNDILREVV VKPGELLMVD DPEAVIGRDN TLLQPGEELL
GQVATELRYI QYVESPEGPA LLSRPVVEFA VPSNPDVPST TSVSQQTGRS IQMRAVQRLP
YKDSERVKSV EGVELLRTQL VLEIEQEGEQ EHNASPLAAD IELIPDLEDA DVQRLQLVIL
ESLVLRRDIA ADATQGSTQT SLEVKDGDTI VPGSVVARTQ ILSKEGGIVR GVQKGSEAVR
RCLVLRHSDM ATLNISAKPK VKAGDLIVAG TELAPGIFAE ESGQIVGVKN AGESTTTQDA
ALSTQNYAVT IRAGRPYRVS PGAVLQIEDG DLVQRGDNLV LLVFERAKTG DIIQGLPRIE
ELLEARKPKE ACILAKRGGE VKVVYGDGDE AIAIKVIESN GVVTDYPLGP GQNLAMPDGS
VVPAGQPLSD GPSNPHEILE VFFSLGSEDG VYACASHALQ KVQTFLVNEV QMVYQSQGID
IADKHIEVIV RQMTNKVRID DGGDTTMLPG ELVELRQVEQ VNEAMAITGG ARAQYTPVLL
GITKASLNTD SFISAASFQE TTRVLTEAAI EGKSDWLRGL KENVIIGRLI PAGTGYNTYD
EPGMLEDYST LETTSVLDET DDPLDMVLDD RTARAYNLDS PGLAETGFNN RRSILDDDEL
IADEIHDLVE AEVEVDDEVD DDYEDDDEDD DDYED
