RPOC2_OENAR
ID RPOC2_OENAR Reviewed; 1383 AA.
AC B0Z4M7;
DT 04-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT 08-APR-2008, sequence version 1.
DT 03-AUG-2022, entry version 51.
DE RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324};
OS Oenothera argillicola (Appalachian evening primrose).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Myrtales; Onagraceae; Onagroideae; Onagreae; Oenothera.
OX NCBI_TaxID=3940;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Douthat 1;
RX PubMed=18299283; DOI=10.1093/nar/gkn081;
RA Greiner S., Wang X., Rauwolf U., Silber M.V., Mayer K., Meurer J.,
RA Haberer G., Herrmann R.G.;
RT "The complete nucleotide sequences of the five genetically distinct plastid
RT genomes of Oenothera, subsection Oenothera: I. Sequence evaluation and
RT plastome evolution.";
RL Nucleic Acids Res. 36:2366-2378(2008).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC Rule:MF_01324}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR EMBL; EU262887; ABW98705.1; -; Genomic_DNA.
DR RefSeq; YP_001687138.1; NC_010358.2.
DR AlphaFoldDB; B0Z4M7; -.
DR GeneID; 5951922; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Metal-binding;
KW Nucleotidyltransferase; Plastid; Transcription; Transferase; Zinc.
FT CHAIN 1..1383
FT /note="DNA-directed RNA polymerase subunit beta''"
FT /id="PRO_0000353574"
FT BINDING 220
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 289
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 296
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 299
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ SEQUENCE 1383 AA; 156577 MW; 9778FB422B32813D CRC64;
MDEGVNLVFP KKVIDGTAIK RLISRLIDHF GMAHTSHILD QVKTLGFQQA TATSISLGID
DLLTIPSKGW LVQDAEQQSL SLEKHHHYGN VHAVEKLRQS IEVWYATSEY LRQEMNPNFR
MTDPFNPVHI MSFSGARGNA SQVHQLVGMR GLMSDPQGQM IDLPIQSNLR EGLSLTEYII
SCYGARKGVV DTAVRTSDAG YLTRRLVEVV QHIVVRRTDC GTLRGISVSP RRMPERIFIQ
TLIGRVLADD IYIGSRCIAI RNQDIGIGLV NRFITFRIQP ISIRTPFTCR STSWICRLCY
GRSPTHGDLV ELGEAVGIIA GQSIGEPGTQ LTLRTFHTGG VFTGGTAEHV RAPSNGKIKF
NFNEALVHPA RTRHGHPALL CSMDLDVTIE SEDILHNLTI PPKSFLLVQN NQYVESEQVI
AEICAGTSTF HFKERVRKHI YSDSEGEMHW STDVYHAPEF TYSNVHLLPK TSHLWILSGG
SCRSRGAPFS LHKDQDQMNP RSTERERRYL SSLSANNDQI RYKFFSSSFS GKKKDDRSPG
YSEMNRIICT LPCNLIYPSI LRENSDLLAK RRRNRLVIPV QSSQEREKEL IPHSGISIEL
PINGIFRKKS ILAFFDDPRY RTKSSGITQY ETMGMHSIVK KEGLVDYRGI NEFKPKYQMT
IDRFFFIPEE VHILPESSSI MVRNNSLIGV DTRIALNTRS RAGGLVRVER KKRGIALQIF
SGTIHFPGET DKISWDSGIL IPPGTGKRNS KESKKWKNGI YVQRITPTKK KHFVLFRPVV
TYEIADGLNL ARLFPPDLCQ EKDNMQLQIV NYIVYGNGKP IREISDTSIQ LVRTWFILNW
DQGKKSASAE AAHASFVEVR AKGLIRDFLR IDLVKSPILD PRKRNDPSGS GLISDNVSDH
TNINPFYSKP KMKQSPRQNH GTIRTLLNQN KECPSLMILS ASNCFRMGPF NDVKSQNVIK
ESIKKDAIIQ IRNSIGPLGT ALQVVNFDSF YYFITHNQVL LTKYLQVENL KQTFQVLQYY
LMDESGRIYN PDPRSNIVLN SFNLSWYFLP HNNYENSCEE ISTIVSLGQF ICENGCIAKN
GPYLRSGQVL IVRLDSVVIR SAKPYLATPG ATVHGHYGEI LYDGDTVVTF LYEKSRSGDI
TQGLPKVEQV LEVRSVDSIS VNLEKRVENW NEHITRILGF PWGFLIGAEL TIVQSRISLV
NKIQKVYRSQ GVQIHNRHIE IIVRQITSKV LVSEDGMSNV FLPRELIGLL RAERTGRALE
ESICYKAFLL GITRTSLNTQ SFISEASFQE TARVLAKAAL RGRIDWLKGL KENVVIGGMI
PVGTGFKGLV HCSKQHKSIP KNKHFFEGEI RDILFHHREL FDSCISKNFH DTPEQSFRVF
NDS
