ID   RPOC2_OLTVI             Reviewed;        1859 AA.
AC   Q20EX3;
DT   04-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT   18-APR-2006, sequence version 1.
DT   03-AUG-2022, entry version 56.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE   AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE            Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN   Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324};
OS   Oltmannsiellopsis viridis (Marine flagellate) (Oltmannsiella viridis).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Chlorophyta; Ulvophyceae; Oltmannsiellopsidales;
OC   Oltmannsiellopsidaceae; Oltmannsiellopsis.
OX   NCBI_TaxID=51324;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16472375; DOI=10.1186/1741-7007-4-3;
RA   Pombert J.-F., Lemieux C., Turmel M.;
RT   "The complete chloroplast DNA sequence of the green alga Oltmannsiellopsis
RT   viridis reveals a distinctive quadripartite architecture in the chloroplast
RT   genome of early diverging ulvophytes.";
RL   BMC Biol. 4:3-3(2006).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01324}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC       Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01324}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC       Rule:MF_01324}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR   EMBL; DQ291132; ABB81940.1; -; Genomic_DNA.
DR   RefSeq; YP_635872.1; NC_008099.1.
DR   AlphaFoldDB; Q20EX3; -.
DR   GeneID; 4100143; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.132.30; -; 1.
DR   Gene3D; 1.10.274.100; -; 1.
DR   HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR   InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR   InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR   InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR   InterPro; IPR007083; RNA_pol_Rpb1_4.
DR   InterPro; IPR007081; RNA_pol_Rpb1_5.
DR   InterPro; IPR038120; Rpb1_funnel_sf.
DR   PANTHER; PTHR19376; PTHR19376; 4.
DR   Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR   Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR   TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE   3: Inferred from homology;
KW   Chloroplast; DNA-directed RNA polymerase; Metal-binding;
KW   Nucleotidyltransferase; Plastid; Transcription; Transferase; Zinc.
FT   CHAIN           1..1859
FT                   /note="DNA-directed RNA polymerase subunit beta''"
FT                   /id="PRO_0000353579"
FT   BINDING         286
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT   BINDING         359
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT   BINDING         366
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT   BINDING         369
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ   SEQUENCE   1859 AA;  205417 MW;  DEB82C70C1F2823C CRC64;
     MNLNFSLACE TQASSFYGGF RPFIKYPSLQ HSPGSVKEGD NKGANRSLKK ATPWLRVSLE
     RPYKYRTTLN ARPVFFNRCF DKGRFKSLIL WCILNFGEQK TVELVEQLKD IGYHQATLAG
     VSLGIDDLKI PNSKAQLVAE AQLENQLILQ EDLRGNVTSI EKFQRLIDTW HLTSETLKDN
     VIKAFRNTDI LNPVYMMAFS GARGNVSQVR QLVGMRGLMA DPQGQIINFP IQSNFREGLT
     LTEYVISCYG ARKGVVDTAL RTATSGYLTR RLVDVAQHVI VYQFDCQTTR GILLNTIKVG
     NTTSISLQKR LVGRVSAEDI YNSKTNTKEV SRNQEITPIL AEQISQNHNQ VLVRSALTCQ
     AKHGVCQLCY GWSLAQGSLV SLGEAVGVVA AQSIGEPGTQ LTMRTFHTGG VFSGDVMEQI
     QAPHNGIVEF QTALQGTLIR TSHGKIAFLT YTKGELTLKN TTKAQRGLNH HLSSSLTQTV
     VGETKIIFPS YTILYVRNGE AVKHKQFIAE YSSLVGENQS IKSSQKVNAE MTGEVFFENV
     FLRVESSEES EKTYRSFKFS DIWILSCVTN TLKFKSNFSL TKPGDRINTA TVINQVLLKK
     TDEGVATQQQ SKKALSLPLN TSMEVLGEAN KVDFSSKKTC KGLLQTDYKN LGYFYSFQNK
     LTKKAKSFDH STSHLSRKGT VSLVQKNNTK SVNELFFKYG KPNLSGGSDL FKGSTSKGSP
     EGATGSLPVS SLNKDPFVTW YSSLFKAKYS GFYTPSTAPF TDTRMSQPAF RLAKKTANKK
     PPTLPCKYWG KGSFLLRNLS EVELFSNRNP GVFKRLESMT GLLSQQVYQD CYANQKILLT
     PFSPNTLQHS SPNTPKGVLR DGKSVVGRSN PPQHFHGSVG GGCPSKQVSA GPKKGLRAKH
     SEACFAIDFG ETKTKTITKG RYVSQLNCKK EKRLLAKTSR MFSTLKVEFW LFENLTLSLV
     THQHFPQYSY RSVEGPPTGM LGLWLLNNSF VLKQLVTNLE TVASVGLPFG VVLNQNTNQN
     LNGSELLSQS LLNSSKKVEG PGRSASSGVL RAGSVGVDWL YFSSANTYLG KVSSGFENNK
     TQLIKSGQGV KQNFDQSLIS QETILLASAL KQLSNKQKTW PSLAFYNTLT FKNNNELGQS
     QLFNTGELDN NASNKSSVFS TGKSLLCHNP VLSKICNTTT FITPFNIKDR GVAPTELQLK
     QENKCIATQS SFLCNPTNLT PKVFLTTNYP GKVACSCVGG DVGKDTFVKV NLSQFDFIES
     NQSRFVPTLS FTSTLNRVNA VQQISLTKPH IYQKFFLSAQ RGSYHLKTKK TLQGSPGLSY
     SGISNYSVSH HRSKKAESHT LPLRLNYAVK KVSRVDEERI PLRKKPKTDI TKVFSIPELD
     WNVKLRLPSI NSPETQGKNK AGVSYLVKLD LGVQRPQTAV SGRVDIFQRG LGDSEKALGF
     KGVAPHSSEK LNVPVFNQTN IFGLADSDAS VKAGAVFETS KYTGEILKSI ESKQQRLFNT
     PLGVVEKGLQ LQNGQTHLSQ LETKTIPSSL SPDSTQISPT QRVLTSDDQK TFNVLGKKPL
     VKVGDFVRYG DFLTCDKKIS VSEPGLVIKI TSSKITIRIA KPVLVSSGGV FHVQHGDFIE
     ENAPLVTLTY TRLKTGDIVQ GIPKIEELFE ARISGTLHNQ LAIIFENYKQ KFSSAYAARK
     SLERIQQIIV ENVLNVYQSQ GVTIADKHVE IIVRQMTSKV RILESGRSGL LRGELVTLES
     VENANKSIHG QKAEYAPVLV GITKAALDID KSFISAASFQ ETTRILSRAA IERKTDFLRG
     LKENVILGQL IPAGTGFSVS FSPEDPNHSK KVVKLVNQYL FSSDNLTSST FSHMSRSSH