RPOC2_PANGI
ID RPOC2_PANGI Reviewed; 1387 AA.
AC Q68S16;
DT 21-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 62.
DE RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324}; ORFNames=PSC0178;
OS Panax ginseng (Korean ginseng).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; campanulids; Apiales; Araliaceae; Panax.
OX NCBI_TaxID=4054;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15500250; DOI=10.1093/dnares/11.4.247;
RA Kim K.-J., Lee H.-L.;
RT "Complete chloroplast genome sequence from Korea ginseng (Panax schinseng
RT Nees) and comparative analysis of sequence evolution among 17 vascular
RT plants.";
RL DNA Res. 11:247-261(2004).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC Rule:MF_01324}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR EMBL; AY582139; AAT98499.1; -; Genomic_DNA.
DR RefSeq; YP_086956.2; NC_006290.1.
DR AlphaFoldDB; Q68S16; -.
DR PRIDE; Q68S16; -.
DR GeneID; 3021491; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Metal-binding;
KW Nucleotidyltransferase; Plastid; Transcription; Transferase; Zinc.
FT CHAIN 1..1387
FT /note="DNA-directed RNA polymerase subunit beta''"
FT /id="PRO_0000067938"
FT BINDING 224
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 295
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 302
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 305
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ SEQUENCE 1387 AA; 157337 MW; 57DBFC7337945F33 CRC64;
MEVLMAERAN LVFHNKVIDG TAMKRLISRL IEHFGMAYTS HILDQVKTLG FQQATATSIS
LGIDDLLTIP SKRWLVQDAE QQSLILEKQH HYGNVHAVEK LRQSIEIWYA TSEFLRQEMK
PNFRMTDPFN PVHIMSFSGA RGNASQVHQL VGMRGLMADP QGQMIDLPIQ SNLREGLSLT
EYIISCYGAR KGVVDTAVRT SDAGYLTRRL VEVVQHIVVR RKDCGTVRGI SVSPRNGMMP
ERVFIQTLIG RVLADDIYLG PRCIATRNQD IGSGLVNRFI TFRAQPIYIR TPFTCRSTSW
ICRLCYGRSP THGDLAELGE AVGIIAGQSI GEPGTQLTLR TFHTGGVFTG GTAEHVRAPS
NGKIKFNEDL VHPTRTRHGH PAFLCSIDLY VTIESEDIIH NVNIPPKSFL LVQNDQYVES
EQVIAEIRAG TSTLNFKEKV RKHIYSDSEG EMHWNTDVYH APAFTYGNVH LLPKTSHLWI
LLGEPCRSDL VALSIHKDQD QMNAHSLSVK RRYISNLSVT NDQARHKFFS SDFSGKKEDR
ITDYSELNRI GHCNLPYLAI LHANSDLLAK RRRNRFIIPL QSIQEHENEL MPSSGISIEI
PIHGIFRKKS IIAYFDDPRY RRKSSGITKY GTIEVHSIVK KEDLIEYRGV KEFRPKYQMK
VDRFFFIPEE VHILPGSSSI MVRNNSIIGV DTQITLTTRS RVGGLVRVER KKKRIELKIF
SGDIHFPGET DKISRHSGVL IPPGTGKTNS KESKKWKNWI YVQRITPTKK KYFVLVRPVV
TYEITDGINL ATLFFPDLLQ ERDNVQLRVV NYILYGTGKP IRGFYDTSIQ LVRTCLVLNW
VQDKKSSSIE EARTSFVEIR INSLIRDFLK IDLAKSPILY TGKRKDPSGS GLISENGSDR
TNINPFSSSF FYSKARIKES LNQNQGTIHT LLNRNKECQS LIILSSSNCF RMGPFNDVKY
HNVIKESIQK DPLIQIRNSL GPLGTALQIA NFYSFSHLIT YNQILVTNYL QLDNLKQSFQ
VLKYYLMDEN GKIYNPDRCC NIILNPFNLN WFFLHHNYCE ETSTTMSLGQ FICENVCIAK
NGPHLKPGQV LIVQVDSVVI RSAKPYLATP GATVHGHYGE ILYEGDTLVT FIYEKSRSGD
ITQGLPKVEQ VLEVRSIDSI SMNLEKRVEG WNECITRNLG IPWGFLIGAE LTIVQSRISL
VNKIQKVYRS QGVQIHNRHI EIIVRQITSK VLVSEDGMSN VFLPGEFIGL LRAERMGRAL
EEAICYQAVL LGITKASLNT QSFISEASFQ ETARVLAKAA LRGRIDWLKG LKENVVLGGM
IPVGTGFKGL VPPSRQHKNI PLETKNKNLF EGEMRDILFH HRKFFDSCLS KNFHDTPEQS
FIGFNDS
