RPOC2_PAUCH
ID RPOC2_PAUCH Reviewed; 1372 AA.
AC B1X3M8;
DT 04-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT 20-MAY-2008, sequence version 1.
DT 03-AUG-2022, entry version 49.
DE RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=DNA-directed RNA polymerase subunit beta' {ECO:0000255|HAMAP-Rule:MF_01324};
DE Short=RNAP subunit beta' {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=RNA polymerase subunit beta' {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=Transcriptase subunit beta' {ECO:0000255|HAMAP-Rule:MF_01324};
GN Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324}; OrderedLocusNames=PCC_0095;
OS Paulinella chromatophora.
OG Plastid; Organellar chromatophore.
OC Eukaryota; Sar; Rhizaria; Imbricatea; Silicofilosea; Euglyphida;
OC Paulinellidae; Paulinella.
OX NCBI_TaxID=39717;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=18356055; DOI=10.1016/j.cub.2008.02.051;
RA Nowack E.C.M., Melkonian M., Gloeckner G.;
RT "Chromatophore genome sequence of Paulinella sheds light on acquisition of
RT photosynthesis by eukaryotes.";
RL Curr. Biol. 18:410-418(2008).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- SUBCELLULAR LOCATION: Plastid, organellar chromatophore.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR EMBL; CP000815; ACB42547.1; -; Genomic_DNA.
DR RefSeq; YP_002048757.1; NC_011087.1.
DR AlphaFoldDB; B1X3M8; -.
DR GeneID; 6481180; -.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0070111; C:organellar chromatophore; IEA:UniProtKB-SubCell.
DR GO; GO:0009536; C:plastid; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 4.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 1.
DR TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Metal-binding; Nucleotidyltransferase;
KW Organellar chromatophore; Plastid; Transcription; Transferase; Zinc.
FT CHAIN 1..1372
FT /note="DNA-directed RNA polymerase subunit beta''"
FT /id="PRO_0000353587"
FT BINDING 252
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 321
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 328
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 331
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ SEQUENCE 1372 AA; 151182 MW; 539EED1ECEE78599 CRC64;
MTPSSNSVKT SRFNKVRTDI IGDTDLPNNL IKSLSKSPPH FYNHIIDKKG LRDIIAWAYK
SHGIAATAEL ADDIKDLGFK YATVAAVSIS IDDLKIPKEK KFLLEQAEEQ ITATEERYRL
GEITEVERHT KVIDTWTETN ERLVLAIKKN FNENDPLNSV WMMANSGARG NISQVRQLVG
MRGLMANPQG EIIDLPIRAN FKEGLTVTEY VISSYGARKG LVDTALRTAD SGYLTRRLVD
VAQDVIVRED DCGTERGIVV EADEKGNYGT KLVGRLAAQS VVVDNENRVL VRRNREIDLI
TACRIEAADI PSVIVRSPLT CEAARSVCRK CYGWALAHNA LVDLGEAVGI IAAQSIGEPG
TQLTMRTFHT GGVSTAETGL VRSTLEGSII FGSKARVRPY RTPHGVEAQQ AETDFVLQVK
PNNGKKSQKV DITNGSLLFV SDSQEVASDT ILAQIISGSS VKKSVEKATK DVICDLAGQV
RYDEALQPKE VIDRQGSATH KATRLGRVWV LSGDVYNLPP NAKPVIQGNS KVEEGEVLAE
SRLSSEHGGV VRLRESTGDS REVQIVTTSM TLKDFKLLGE STHSGEIWHL EAKDTTRYLL
KTQPGSKIGN GEVIAELADE RFRTKTGGLV KYAPGGLSIK KARSAKNGYE VNKGGTLLWI
PQETHEINKD ISLLMIEDGQ WIEDKTEVVK DIFSQIAGIV TVTQKNDILR EITVRPGTLY
PCNESKVIER FKGEGLLVDE EEIISKNLKA EKRVFVQSVE TSEGPQLLIR PVEEYTIPEV
AHLPELAIVK QNSGPYLGLK ATQRLNFKDG ELIKSVEGVE LLKTQLILET FDTTPQMTVD
VEKVTDKRSK TLERLQLVIL ETLLVRRDTI SDASHGSTHT ELQVNDGDLV KSGDVVATTQ
ILCKEVGIVQ LPKQIDNEPI RRIIVERDQD TMTIPLGSAP LVSVGQRLVD GDLLAENDPS
PCCGQVETIK NNTIIIRIGR PYMISSDSTL HVKDRELVQR GDSLALLVFE RQKTGDIVQG
LPRIEELLEA RRPRDSAVLC KQPGVLTLRH DEETDSSIAV IKSANGEETE YPILLGRNLM
VSVGQHIKAG ELLTDGPINP HELLEYLFAD LRSRKPLMEA AREAIAKVQS RLVTEVQNVY
KSQGVTIHNK HIEVIVRQMT SKVRIEDAGE TTLLPGELIE LRHVEKVNKA MFMTSSSPAV
FIPELLGITK ASLNTDSFIS AASFQETTRV LTEAAIEGKT DYLRGLKENV IIGRLIPAGT
GFSGFEEELR SEAGPHPDIL DEDPAGYRRM QNLRPDYTVD MPAAASASPA ALLDDPSDDE
LEATRSRHGI EVTTSNTAAF TRPVIVNKIM EDQILDPDII ASLQEEGLLT RE
