ID   RPOC2_PHAAO             Reviewed;        1388 AA.
AC   Q3BAQ2;
DT   07-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT   22-NOV-2005, sequence version 1.
DT   03-AUG-2022, entry version 54.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE   AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE            Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN   Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324};
OS   Phalaenopsis aphrodite subsp. formosana (Moth orchid).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Asparagales; Orchidaceae;
OC   Epidendroideae; Vandeae; Aeridinae; Phalaenopsis.
OX   NCBI_TaxID=308872;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Taisugar TS-97;
RX   PubMed=16207935; DOI=10.1093/molbev/msj029;
RA   Chang C.-C., Lin H.-C., Lin I.-P., Chow T.-Y., Chen H.-H., Chen W.-H.,
RA   Cheng C.-H., Lin C.-Y., Liu S.-M., Chang C.-C., Chaw S.-M.;
RT   "The chloroplast genome of Phalaenopsis aphrodite (Orchidaceae):
RT   comparative analysis of evolutionary rate with that of grasses and its
RT   phylogenetic implications.";
RL   Mol. Biol. Evol. 23:279-291(2006).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01324}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC       Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01324}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC       Rule:MF_01324}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR   EMBL; AY916449; AAW82493.1; -; Genomic_DNA.
DR   RefSeq; YP_358566.2; NC_007499.1.
DR   AlphaFoldDB; Q3BAQ2; -.
DR   PRIDE; Q3BAQ2; -.
DR   GeneID; 3741668; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.132.30; -; 1.
DR   Gene3D; 1.10.274.100; -; 1.
DR   HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR   InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR   InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR   InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR   InterPro; IPR007083; RNA_pol_Rpb1_4.
DR   InterPro; IPR007081; RNA_pol_Rpb1_5.
DR   InterPro; IPR038120; Rpb1_funnel_sf.
DR   PANTHER; PTHR19376; PTHR19376; 1.
DR   Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR   Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR   TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE   3: Inferred from homology;
KW   Chloroplast; DNA-directed RNA polymerase; Metal-binding;
KW   Nucleotidyltransferase; Plastid; Transcription; Transferase; Zinc.
FT   CHAIN           1..1388
FT                   /note="DNA-directed RNA polymerase subunit beta''"
FT                   /id="PRO_0000225336"
FT   BINDING         224
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT   BINDING         294
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT   BINDING         301
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT   BINDING         304
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ   SEQUENCE   1388 AA;  158314 MW;  038FA81DEC3621F0 CRC64;
     MEVLMAERTN LVFHNKVIDG TAIKRLISRL IDHFGMAYTS HILDQVKALG LRRATATSIS
     LGIDDLLTIP SKRWLVQDAE QQSFLLEKNH HYGNVHVVEK LRQSIEVWYA TSEYLRQEMN
     PNFQMTDPSN PVHLMSFSGA RGNASQVHQL VGMRGLMSDP QGQMIDLPIQ SNLREGLSVT
     EYIISCYGAR KGVVDTAVRT SDAGYLTRRL VEVVQHILVR RTDCGTIRGI SVSPQNGMTE
     KIFVQTLIGR VLADDIYIGL RCIGTRNKDI GIGLVNRFIT FQAHPIYIRT PFTCRSTSWI
     CQLCYGRSPT HGDLVELGEA VGIIAGQSIG EPGTQLTLRT FHTGGVFTGG TAEHVRAPSN
     GKIKFDEGLV HPTRTRHGHP AFLCFIDFYV TIESRDIRHN VNIPTKSLIL VQNDQYVESE
     QVIAEIRAET STFHFKERVQ KHIYSESEGE MHWSTDGYHT PKYPYSNVHL LPKTSHLWIL
     AGGLCRSNSN IVSFSLHKDQ DQMNANSFSL KDLFDLSLTN DQVRHKLLDT FGKKDRESLD
     YSKPYRIISK GHWNLIEPST YIRQENSLAK KRRNRFVIPL QYDQEQENKL IPCFGISIKI
     PINGILRRNS IIAYFDDPRY RRSSSGITKY GIVEVDSIVK KEDLIEYRGA KDFSPKYQTQ
     MKVDQFFFIP EEIHILPGSS PIMVRNNSII GVDTRLVLNI NTRSRVGGLV RVERKKKSLE
     LKIFSGDIHF YGEVDKISRH SGILIPPGIG KKDSKGSKKL KNWIYVQRIT PTKKKYFVLV
     RPVVTYEIAD GINLATLFSQ DLLQEKNNVQ LRIVNYILYE NGKSIRGISH TSIQLVRACL
     VLTWDQEKKS SIEEKVHASF VEIRTNDLLC YFIRIELVKS TLSYTEKRYD TAGSGLISNN
     ELDRIHRKNP FDSKAKIQIF PQHQGTLGTL LNRNKECQSF LILSSSNCSR IGPFNTSRYN
     NATKELDPMT PIRYLLGPLG TIVPRIVNSR SSYYLRTYNQ VFLKKSFLLE NFQQTFQVLQ
     VPPFQYCFLD ENSRIYNPDP CSNIIWNSFH LNWCFLHHDS CEEAWPIISL GQFLCENVYL
     LKYGSRIKKS GQIFIVHVDS LVIRSAKPYL VTPGATVHGH YGETFYEGDT LITFIYEKSR
     SGDITQGLPK VEQILEVRSI DSISMNLERR VEGWDERIRR ILGIPWGFLI GAELTIAQSR
     ISLVNKIQKV YRSQGVQIHN RHLEIIVRQV TSRVLVSEDG MSNVFLPGEF IGLLRAERAG
     RVLAESICYR AILLGITKSS LNTQSFISEA SFQETARVLA KAALRGRIDW LKGLKENVVL
     GGIIPVGTGF NKLVHRSKQD KNIHLKIKRK NLFELEMSDI LLHHRELFCS CAQELSMRHQ
     TNLLRNVS