RPOC2_PINTH
ID RPOC2_PINTH Reviewed; 1224 AA.
AC P41606;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 2.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324};
OS Pinus thunbergii (Japanese black pine) (Pinus thunbergiana).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Pinopsida; Pinidae; Conifers I; Pinales; Pinaceae; Pinus;
OC Pinus subgen. Pinus.
OX NCBI_TaxID=3350;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=7937893; DOI=10.1073/pnas.91.21.9794;
RA Wakasugi T., Tsudzuki J., Ito S., Nakashima K., Tsudzuki T., Sugiura M.;
RT "Loss of all ndh genes as determined by sequencing the entire chloroplast
RT genome of the black pine Pinus thunbergii.";
RL Proc. Natl. Acad. Sci. U.S.A. 91:9794-9798(1994).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC Rule:MF_01324}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR EMBL; D17510; BAA04325.1; -; Genomic_DNA.
DR PIR; T07446; T07446.
DR RefSeq; NP_042367.1; NC_001631.1.
DR AlphaFoldDB; P41606; -.
DR GeneID; 809025; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 2.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Metal-binding;
KW Nucleotidyltransferase; Plastid; Transcription; Transferase; Zinc.
FT CHAIN 1..1224
FT /note="DNA-directed RNA polymerase subunit beta''"
FT /id="PRO_0000067942"
FT BINDING 233
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 308
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 315
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 318
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ SEQUENCE 1224 AA; 139272 MW; DCD355D333A4601B CRC64;
MKIWRFFLMK ERTRLPFDNL PFYNKVMDKT AIKKLISRLI DHFGMTYTSH ILDQLKTSGF
QQATDTAISL GIDDLLTAPS KGWLVQDAEQ QGSVSEKQNH YGNVHAVEKL RQSIEIWYAT
SEYLRKEMNP NFSMTDPLNP VHVMSFSGAR GSTSQVHQLV GMRGLMSDPQ GQIIDLPIRR
NLREGLSLTE YIISCYGARK GVVDTAVRTA DAGYLTRRLV EVVQHIVVRR TDCGTIQGIF
VSPIRGRERD RNEIVVRTQI LIGRVLADDV YINRRCIATR NQDIGVGLAN QLINLRTQPI
YIRTPFTCKS ISRICQLCYG RSTTHSHLIE LGEAVGIIAG QSIGEPGTQL TLRTFHTGGV
FTGDIAEHIR APFNGKIEFN ENLVYPTRTR NGHPAYLCHN NLSITIDGQD QVQNLTIPPQ
SLLLVQNDQY VESEQLIAEV RARTSSFKEK VRKNIYSDLE GEMHWSTNVC HAPEYVQGNV
HPILRTGYLW ILSGGIYGSR VVPFPFHKYQ DQVYVQPFVA KHQSLSDSYV DQVEHRSGDS
NCYEKEEQIF SYSETETDRT ISNKHRDSIY VFYPNNYNIK GKKQMNRFIV SLQCDKEWEK
KIIPCPDAIL RIPKSGILQR NSIFGYSNVE HGIPDGSNMT TPFSLDLSRE GDNLQIQISY
SISYEDGERI QVMSDISIPL VRTCIGFDWE QIDSIESEAY VSLISVRTNK IVNNMVQISL
MKYPPFFMGR RDNKTSPNLM FHNNLDHTNL LSSNGASQLI SKHQGIICSL SNGEEDSGSF
MVLSPSDYFR IVLFNDSKCY DTGNQSNRKD PMRKIIEFSG LLGNLHSITN RFPSSHFLTY
KKVLSKKHSI FHNSFNTFQV PKYYFMDENM IIYHFDPCRN IISNLLGPNW CSSSSESEFC
EKTFPVVSLG QLIPESVWIS EDEPLPESGQ IIAVDEESLV IRSAKPYLAT RKATVHSHYG
KILDKGDTLI TLIYERFKSS DIIQGLPKVE QLSEARLNNS ISMNLKESFE NWTGDMTRFL
GSLWGLFISA RITMEQSQIH LVNQIQKVYR SQGVRIGDKH IEVIVRQMTS KVLISEDGTA
NVFSPGELIV LSRAQRMDRA LEEAIYYQTM LLGITRASLN TQSFISEASF QETARVLAKA
ALQGRIDWLK GLKENVILGG MIPAGTGQHI HRSGKRNGID PRIGNRNLFS NKVKDILFHH
DKVDFFSFQD NSYKYHNILK QQLK
