RPOC2_PIPCE
ID RPOC2_PIPCE Reviewed; 1380 AA.
AC Q06GS0;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 31-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 53.
DE RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324};
OS Piper cenocladum (Ant piper).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Magnoliidae; Piperales; Piperaceae; Piper.
OX NCBI_TaxID=398741;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=17020608; DOI=10.1186/1471-2148-6-77;
RA Cai Z., Penaflor C., Kuehl J.V., Leebens-Mack J., Carlson J.E.,
RA dePamphilis C.W., Boore J.L., Jansen R.K.;
RT "Complete plastid genome sequences of Drimys, Liriodendron, and Piper:
RT implications for the phylogenetic relationships of magnoliids.";
RL BMC Evol. Biol. 6:77-77(2006).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC Rule:MF_01324}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR EMBL; DQ887677; ABI14462.1; -; Genomic_DNA.
DR RefSeq; YP_784463.1; NC_008457.1.
DR AlphaFoldDB; Q06GS0; -.
DR GeneID; 4363711; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Metal-binding;
KW Nucleotidyltransferase; Plastid; Transcription; Transferase; Zinc.
FT CHAIN 1..1380
FT /note="DNA-directed RNA polymerase subunit beta''"
FT /id="PRO_0000277198"
FT BINDING 225
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 295
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 302
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 305
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ SEQUENCE 1380 AA; 156314 MW; C08D24F7F09BF0F9 CRC64;
MEVLMAEQRA DLVFHNKAID GTAMKRLISR LIDHFGMAYT SHILDQVKTL GFQQATATSI
SLGIDDLLTI PSKGWLVRDA EQQGLILEKH HHYGNVHAVE KLRQSIEIWY ATSEYLRQEM
HTNFRMTDPS NPVHIMSFSG ARGNASQVHQ LVGMRGLMSD PQGQMIDLPI QSNLREGLSL
TEYIISCYGA RKGVVDTAVR TSDAGYLTRR LVEVVQHIVV RRTDCGTIRG ISASPRNSME
EKIWIQRLIG HVLAYDIYIG PRCIAARNQD IGIGLVNRFI TFQAKSIYIR TPFICRSTSW
ICRLCYGRSP THGDLVELGE AVGVIAGQSI GEPGTQLTLR TFHTGGVFTG GTAEYVRAPS
NGKIKFHEDL VHPTRTRHGH PAFLCYIDLY VTIESRDILH KVNIPPKSFI LVQNNQYVES
EQVIAEIRAG TSALSFKEKV RKHIYSDSEG EMHWSTNVYH TPQYTYGNVH LLPKTSHLWI
LSGNPCKSNG ALFSLHKDQD QMNLYSLSLS LEQRSISDFS VTNDRVRRKS FSLDPSREGR
GGRFFDYSVP DKSIPNDCWN FIYSAILHEN SYFLAKRRRN RFIIPFQYSQ EGEKEPMPCS
GISIEIPKTG ILRRNTILAY FDDPRYRRSG SGITKYGTVE VDSLVKKEDL VEYRGEKELR
TKYQTKVDRF FFIPEEVHIL PGSSSIMVRN NSIIGVDTEI ALNTRSRVGG LVRVERKKKR
TELKIFSGNI HFPGETDKIS RHSGILLPPG MEKKNSKESK KWKNWIYVQR VTPIKKKYFV
SVRPVVTYEI ADGINLATLF PQDSLQERDN VQLRVVNYIL YGNGKPIRGI SHTSIQLVRT
CLVLNWDQDN KKTSSIEEVF ASFVEVRAND LIRDFIRVDL VKPRVLNTGK RNDTAGYGLN
PDNGSDCPNR NPFYSKIKIQ SFTQDNGTIH TSLNINKQLP PFLILSSSNC FQAGPLKVNG
SKSDNGKKKS IKEDPAILIR NFFGPLGTIA KITNLDSRNY YLTDNQILLK KYLILDNFKN
LFQDVLYYLI DEKGRILNPD PCSNIILNAF DLNWCFLHHN SCEKTFSIIS LGQLFYENGS
IYKYRSHIKS GQIVTVHGDS LVIRSAKLHL ATPGATVHGH YGEIIYEGDT LVTFIYEKSR
SGDITQGLPK VEQVLEVRSI DSISMNLEKR VEGWNENITK ILGIPWGFLI GAELTIVQSR
ISLVNKIQKV YRSQGVQIHN RHIEIIVRQI TSKVLVSEDG MSNAFSPGEL IGLLRAERTG
RALEETICYR AILLGLTRAS LNTQSFISEA SFQETARVLA KAALRGRIDW LKGLKENVVL
GGMIPVGTGF KGFVRRSRQH NNISLEKKRN NLVKGEIGNI LFHHRELFSS CISKNKKKFS
