RPOC2_PLAOC
ID RPOC2_PLAOC Reviewed; 1387 AA.
AC Q09G56;
DT 04-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT 17-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 58.
DE RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324};
OS Platanus occidentalis (Sycamore) (American plane tree).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Proteales; Platanaceae; Platanus.
OX NCBI_TaxID=4403;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16934154; DOI=10.1186/1471-2229-6-17;
RA Moore M.J., Dhingra A., Soltis P.S., Shaw R., Farmerie W.G., Folta K.M.,
RA Soltis D.E.;
RT "Rapid and accurate pyrosequencing of angiosperm plastid genomes.";
RL BMC Plant Biol. 6:17-17(2006).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC Rule:MF_01324}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR EMBL; DQ923116; ABI49768.1; -; Genomic_DNA.
DR RefSeq; YP_740555.1; NC_008335.1.
DR AlphaFoldDB; Q09G56; -.
DR GeneID; 4271287; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Metal-binding;
KW Nucleotidyltransferase; Plastid; Transcription; Transferase; Zinc.
FT CHAIN 1..1387
FT /note="DNA-directed RNA polymerase subunit beta''"
FT /id="PRO_0000353581"
FT REGION 883..903
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 224
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 295
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 302
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 305
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ SEQUENCE 1387 AA; 157374 MW; A9D4C7392E375B65 CRC64;
MEVLMAERAD LVFHNKAIDG TAMKRLISRL IDHFGMAYTS HILDQVKTLG FQQATATSIS
LGIDDLLTIP SKGWLVQDAE QQSLILEKHH HYGNVHAVEK LRQSIEIWYA TSEYLRQEMN
PNFRMTNPSN PVHIMSFSGA RGNASQVHQL VGMRGLMSDP QGQMIDLPIQ SNLREGLSLT
EYIISCYGAR KGVVDTAVRT SDAGYLTRRL VEVVQHIVVR RTDCGTLQGI SVSPRNGMIT
ERILIQTLIG RVLADDIYMG PRCIAARNQD IGIGLVNRFI TFGAQPISIR TPFTCRSISW
ICRLCYGRSP THGDLVELGE AVGIIAGQSI GEPGTQLTLR TFHTGGVFTG GTAEHVRAPS
NGKIKFNEDL VHPTRTRHGH PAFLCYIDLY VTIESQDIIH NVNIPPKSFL LVQNDQYVES
EQVIAEIRAG TSTLNFKERV RKHIYSDSEG EMHWSTNVYH APEYTYSHVH LLPKTSHLWI
LSGGSYRSSI VPLSLHKDQD QMNVHSLSVE RRYISNFSVT NDQVRHKLFN SYPSSKKGER
ILDYSGTDRI ICNGHCNFIY PSILHENSDL LAKRRRNRFI IPFQSNQERE KELMSRSDIS
IEIPINGIFR RNSILAYFDD PRYRRNNSGI TKYGTIEVHA IVKKEDLIEY RRAKEFRPKY
QMKVDRFFFI PEEVHILPGS SSIMVRNNSI IGVDTRITLN ARSRVGGLVR VERKKKRIEL
KIFSGDIHFP GETDKISRHS GILIPPGTGK KNSKKSKKKL QNWIYVQRIT PIKKKYFVFV
RPVVTYEIAD GINLATLFPQ DLLQERDNVQ LRVVNYILYA NGKPIRGISH TTIQLVRTCL
VLNWDQGKNG SSIKEVHASF VEVRANDLIQ DFIRMDLVKS TISHTGKRND PAGSGLIPDN
GSDRTNINPF YSKARIQQSL SRHQGTLRSL LNRNKECQSL IILSSSNCSQ MGPLNTLKYH
NVTKESIKRD PPIPIRNSLG PLGTVPKITN FYSSYYQLIT HNQILVSKYL LLANLKQTLQ
VFKYYLMDEN GKIYNPDPCS NIIFNPFNLN WYFLHHDYCE ETSTIISLGQ FFCENVCISK
KGPHLKSGQV LIVHVDSLVI RSAKPYLATP GATVHGHYGE ILYKGDTLVT FIYEKSRSGD
ITQGLPKVEQ VLEVRSIDSI SMTLENRVEG WNERITRILG IPWEYLIGAE LTIAQSRISL
VNKIQKVYRS QGVQIHNRHI EIIVRQITSK VLVSEDGMSN VFSPGELIGL FRAERTGRAL
EEAICYRAVL LGITRASLNT QSFISEASFQ ETARVLAKAA LRGRIDWLKG LKENVVLGGM
IPVGTGFKGL VHRSREHNNI PLEIKKKNLF EGEMRDTLFH HRELFDSCIP KNFHDTSKQS
FTGFNDF
