RPOC2_PROMM
ID RPOC2_PROMM Reviewed; 1374 AA.
AC Q7V5P3;
DT 16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=DNA-directed RNA polymerase subunit beta' {ECO:0000255|HAMAP-Rule:MF_01324};
DE Short=RNAP subunit beta' {ECO:0000255|HAMAP-Rule:MF_01324};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=RNA polymerase subunit beta' {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=Transcriptase subunit beta' {ECO:0000255|HAMAP-Rule:MF_01324};
GN Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324}; OrderedLocusNames=PMT_1505;
OS Prochlorococcus marinus (strain MIT 9313).
OC Bacteria; Cyanobacteria; Synechococcales; Prochlorococcaceae;
OC Prochlorococcus.
OX NCBI_TaxID=74547;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MIT 9313;
RX PubMed=12917642; DOI=10.1038/nature01947;
RA Rocap G., Larimer F.W., Lamerdin J.E., Malfatti S., Chain P., Ahlgren N.A.,
RA Arellano A., Coleman M., Hauser L., Hess W.R., Johnson Z.I., Land M.L.,
RA Lindell D., Post A.F., Regala W., Shah M., Shaw S.L., Steglich C.,
RA Sullivan M.B., Ting C.S., Tolonen A., Webb E.A., Zinser E.R.,
RA Chisholm S.W.;
RT "Genome divergence in two Prochlorococcus ecotypes reflects oceanic niche
RT differentiation.";
RL Nature 424:1042-1047(2003).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- SUBUNIT: In cyanobacteria the RNAP catalytic core is composed of 2
CC alpha, 1 beta, 1 beta', 1 gamma and 1 omega subunit. When a sigma
CC factor is associated with the core the holoenzyme is formed, which can
CC initiate transcription. {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR EMBL; BX548175; CAE21680.1; -; Genomic_DNA.
DR RefSeq; WP_011130873.1; NC_005071.1.
DR AlphaFoldDB; Q7V5P3; -.
DR STRING; 74547.PMT_1505; -.
DR EnsemblBacteria; CAE21680; CAE21680; PMT_1505.
DR KEGG; pmt:PMT_1505; -.
DR eggNOG; COG0086; Bacteria.
DR HOGENOM; CLU_000524_1_0_3; -.
DR OMA; IEGKSDW; -.
DR OrthoDB; 4373at2; -.
DR Proteomes; UP000001423; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR007066; RNA_pol_Rpb1_3.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 4.
DR Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Metal-binding; Nucleotidyltransferase;
KW Reference proteome; Transcription; Transferase; Zinc.
FT CHAIN 1..1374
FT /note="DNA-directed RNA polymerase subunit beta'"
FT /id="PRO_0000067906"
FT REGION 1..47
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1344..1374
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 12..26
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 258
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 325
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 332
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 335
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ SEQUENCE 1374 AA; 149538 MW; BABF9347E67FCC17 CRC64;
MTSTSPKSRK PSTKTTKSKS KSKSKSKAAK AAAAGASPAL ARTPPQFRNR VIDKKALKQL
VAWAYKTHGT AVTASMADNL KDLGFRYATQ AAVSISVEDL KVPEAKQDLL GQAEAQITAT
EECYRLGEIT EVERHTKVID TWTETNERLV DAVKKNFNQN DPLNSVWMMA NSGARGNMSQ
VRQLVGMRGL MANPQGEIID LPIRTNFREG LTVTEYVISS YGARKGLVDT ALRTADSGYL
TRRLVDVAQD VIVREDDCGT TRGIIVKAED GGFGSRLVGR LTAEQVVNVD GEILAERNTE
IDPPLSKRFE KAAITEVMVR SPLTCEANRS VCRKCYGWAL AHNELADLGE AVGIIAAQSI
GEPGTQLTMR TFHTGGVSTA ETGVVRSTVA GTVEFGPKAR VRGYRTPHGL EAQQSEVDFT
LTVKPSGKGR AQRIDITTGS LLFVSDGQEI EADVTVVQIA AVAVKKSVEK ATKDVICDLA
GQVRYEQVIQ PREVKDRQGN ITLKAQRLGR LWVLAGDVYN LPPNAEPVVQ GNVKVERGQV
LAEASQASEF GGEVRLRDSI GDSREVQIVT TSMTMKDFKL LGESTHSGEL WHLEAKDGTR
YRLNTIPGSK IGNGEVVAEL ADDRFRTQTG GLVRFAPGLA IKKARSAKNG FEVNKGGTLL
WIPQETHEIN KDISLLMIED GQWIEAGTEV VKDIFSQTAG IVTVTQKNDI LREIIVRSGS
FHLCTETKAL ERFTGDGQIV NPGETIAKGI NSEAMVFVQT VDTPEGTGLL LRPMEEYTIP
NEAQLPELTH VKQPKGPHLG IKATQRLAFK DGELIKSVEG VELLKTQLIL ETFDTTPQMT
VDVEAVRDKR AKTIERLRLV ILESILVRRD TISDSSHGST HTELQIEDGQ SVKASDVVAT
TQILCKQEGI AQLPVVQEGD PVRRLIVERD EDTITVTTNG SPLVEVGQRL VDGDSLAKDE
PSSCCGEVEE VDGKAITLRL GRPYMVSPDS VLHVRDGDLV QRGDGLALLV FERQKTGDIV
QGLPRIEELL EARRPRESAV LCKKPGTVEI KQGEDDESIT VTVIEADDAI GEYPILLGRN
VMVSNGQQVH AGELLTDGPI NPHELLDCFF EDLRGRKPLM DAAQEAIAKL QHRLVTEVQN
VYKSQGVSID DKHIEVIVRQ MTSKVRIEDA GDTTLLPGEL IELRQVEDTN QAMAITGGAP
SEFTPVLLGI TKASLNTDSF ISAASFQETT RVLTEAAIEG KSDWLRGLKE NVIIGRLIPA
GTGFSGFQEE LRAEAGPHPD ILAEDPAGYR RMQNLRPDYT VDMPAAPAAS STAVLADPSD
ADLEATRSRH GIDPAASNFA AFVRPTGENE LEEEQLPDPS ALEGLQQEGL LTEE
