RPOC2_PSINU
ID RPOC2_PSINU Reviewed; 1408 AA.
AC Q8WI26;
DT 16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 72.
DE RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324};
OS Psilotum nudum (Whisk fern) (Lycopodium nudum).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Polypodiopsida; Ophioglossidae; Psilotales; Psilotaceae; Psilotum.
OX NCBI_TaxID=3240;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Kingyoku;
RX PubMed=15240838; DOI=10.1093/molbev/msh203;
RA Nishiyama T., Wolf P.G., Kugita M., Sinclair R.B., Sugita M., Sugiura C.,
RA Wakasugi T., Yamada K., Yoshinaga K., Yamaguchi K., Ueda K., Hasebe M.;
RT "Chloroplast phylogeny indicates that bryophytes are monophyletic.";
RL Mol. Biol. Evol. 21:1813-1819(2004).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC Rule:MF_01324}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- CAUTION: The universally conserved zinc-binding site of this subunit is
CC not present in this sequence. {ECO:0000305}.
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DR EMBL; AP004638; BAB84206.1; -; Genomic_DNA.
DR RefSeq; NP_569619.2; NC_003386.1.
DR AlphaFoldDB; Q8WI26; -.
DR SMR; Q8WI26; -.
DR GeneID; 2545169; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Nucleotidyltransferase; Plastid;
KW Transcription; Transferase.
FT CHAIN 1..1408
FT /note="DNA-directed RNA polymerase subunit beta''"
FT /id="PRO_0000067944"
SQ SEQUENCE 1408 AA; 161758 MW; 7A2478C5A4271895 CRC64;
MMVDQIKLLF YNKMMDRTAI KQLISRLIAQ FGITYTTNIL DQLKTLGFKQ ATNASVSLGI
DDLLEAPSKA WLIQDAERQG SIFEQHHRFG SVHAVEKLRQ LIETWYATSE YLKREMIPNF
RIIDPLNPVH MMSFSGARGS TSQVHQLVGM RGLMSDPQGQ IIDLPIRSNL REGLSLTEYI
ISCYGARKGV VDTAVRTSDA GYLTRRLVEV VQHIVVRKMD CGSTRGIPFK MTQDRFKRNL
YQQRLIGRVL ADNVYLEMRC IAMRNQDIGN ELANRLITIQ KQLVYVRSPL TRKSISWVCQ
LCYGWSLTHQ NLVELGEAVG IIAGQSIGEP GTQLTLRTFH TGGVFTGDIA EHIRIPFNGI
ISFAEDSVHP IRTRHGHPAW ICQDNLSVSV KNKNRIHNVI IPYQSLILVQ NNQYVESKQV
IAEVRINQSL PKERVKKHIY SNSEGEMHWS TIVRHSPQHR QSNVYPVLKT GHIWILSGSL
CNVIETSSSF YKEQDRFNIQ SVFTKPEFLP YSFGRNKGEK NQLTNLHKKG QELNHLKFDS
SATIKTYKFP YLTSLFYCKI RKDKTENKVI LSIKPIQRRN KYYRKTPYRD FVFQTPTNGI
LNRGDILAIH ENPEYRIHIS GVIKYGTLKI DSIVENERIP NDREKTTFGS RYKVLRGGNF
FFLPGEIYIV HESSAYILVS NNSIVQAGTQ ITPTLTSQLG GLVQIKNIQK SFEIRILPGT
IHHPKRIPSI SKQNNMLIPP GQSVFDNLKF DHWIYLQWIT SPRKKTFALA RPVIEYFVRK
GSYPPILNLL KEQNTLRVKF LDYMLYEDGE EIQIKNNMSI QLVQTCLLLD WEKKSPIKAA
KTSILELRIN KIIKTFLQIS LLNSFDFYVK GSKFKRFFNN KKSFVADSIP KTLESQLSIK
HQGTIRSVSN RKTSFLVLSP SDSFQNSLST NFQYYDSKNR YGKKKELKWN TFFDTKKVSR
SLKKNYLSSI KDSEKRSLNS KVGLTSVSSF DHTRQLQGMK ILGLLGYLYS IANCFLYPKG
IFRNEVFFHR DSSIDDLVGF DKLPNWYFLD ENRKIYSFHL RNLIGKRFFY WTRNLSNFNE
IPLVNLGQFI CEGARLFENQ ISSQSGQIIA LSPEFLIIRL AKLYLATKGA TIHNHYGDLL
REGDTLITLT YERFKSGDII QGLPKVEQLL EARSVNAVSR NIEDNFKKCN RVIAKFIESP
WSFFFSAKIS TEQSRKDLVD QIQGVYQSQG VQISDKHIEI IVRQMTSKVL TLEDGIATVF
LPGELIELPR AQRMNRALKQ LIYYKPILLG ITKASLNTTS FLSEASFQET TRVLARAAIR
GRIDWLKGLK ENVIIGGIVP TGTGSREVTC QMDLEKHKKG SNLKIKNTHS FSCEIKDLLF
NHGKVSLTFK RSNIHRELKK PLSENDSD