RPOC2_RHDSA
ID RPOC2_RHDSA Reviewed; 1303 AA.
AC A6MVX2;
DT 04-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-JUL-2007, sequence version 1.
DT 03-AUG-2022, entry version 47.
DE RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324};
OS Rhodomonas salina (Cryptomonas salina).
OG Plastid; Chloroplast.
OC Eukaryota; Cryptophyceae; Pyrenomonadales; Pyrenomonadaceae; Rhodomonas.
OX NCBI_TaxID=52970;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CCMP1319 / NEPCC76 / CS-174;
RX PubMed=17522086; DOI=10.1093/molbev/msm101;
RA Khan H., Parks N., Kozera C., Curtis B.A., Parsons B.J., Bowman S.,
RA Archibald J.M.;
RT "Plastid genome sequence of the cryptophyte alga Rhodomonas salina
RT CCMP1319: lateral transfer of putative DNA replication machinery and a test
RT of chromist plastid phylogeny.";
RL Mol. Biol. Evol. 24:1832-1842(2007).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC Rule:MF_01324}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR EMBL; EF508371; ABO70813.1; -; Genomic_DNA.
DR RefSeq; YP_001293551.1; NC_009573.1.
DR AlphaFoldDB; A6MVX2; -.
DR PRIDE; A6MVX2; -.
DR GeneID; 5228635; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 4.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Metal-binding;
KW Nucleotidyltransferase; Plastid; Transcription; Transferase; Zinc.
FT CHAIN 1..1303
FT /note="DNA-directed RNA polymerase subunit beta''"
FT /id="PRO_0000353588"
FT BINDING 225
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 299
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 306
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 309
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ SEQUENCE 1303 AA; 146349 MW; 24BBB73A193A2794 CRC64;
MKEKYLFIPP KPKFTNKTID KKELKKLMAW AFSNYGTGRA SYLADKIKDL GFQYATKAGL
SLSVEDLRVP PTKRELLKRT NEEINLTQQK YERGEITTVE RFQKVIDTWN NASEELKDEV
VKYFKETDPL NTIYIMAFSG ARGNISQVRQ LVGMRGLMAD PQGQIIDLPI KSNFREGLTV
TDYIISSYGA RKGLVDTALR TADSGYLTRR LVDVAQDIII REIDCDTDRG ILLKDMVSNN
QILIPLQNRL LGRVLFETLH SPDSANVIAH INQDLDHNTA EFIVKSGIKS VIVRSPLTCE
SSRSVCQFCY GWNLAHGSLV DLGEAVGIIA AQSIGEPGTQ LTMRTFHTGG VFTGELAEQI
RAPFDGVLRI PKSFRTRLIR TRHGEEAFVL EDSYKLDLYD SSYKKHKIEF KQGTILFLND
NEQFKKSQVI GELSTKSSMI TERVTKDLNT ENSGEVCFSN LYIEEKIDRQ GNSITNTPKG
GCLWILSGEV YNLPSYADIK IKEKQFVEED EVLATSKVIS DYGGLVRLNQ SNQTSNITEL
QIVTSSVSLD NATIVTDDQK STNSDPSYTL EMECGIKFHM LCSPGNKIVN SQIIAELIDT
KYQTSTGGIV KYDGFEVNKK NKNKKGYEIL GEGALLWIPE ETHEINKDIS LLLVEEGQCI
TAGTQIVKEL YSLTEGYIQI IQENEIVKEV IVKPGKEHVR STTSNNLNEY PKIVKPNDPD
YAEYNAQGTI YIEELHYKKG NALLIRPVIE FRIDNEKIDL KTNYLTNENH HITIKPTKRV
LFKDGQRVKS KYGVDLLKTY LIMSVDFDKP HLSADVEFVP AHEDDIYKLH LTVLETLQIK
QDDFGESKKK STITSLCVKQ NELIKAGSTV AETHLLAHSA GFVQSINQTS QSTCKVLILT
NSDEKSIDIY NQTPQVSQGD FIRSGDQIAN GIIAETSGQI VEIETNKIKI RSGTPYLVSS
NAILQVKNGN LVETGDTLAI LVFERSKTGD IVQGLPRIEE ILEARKPKEP SKLSQRPGKI
TLNYDSEDNK CIRILSSNGE YNEYIMSGIQ KIIVSNGENI LLAEPITDGA PNPHEMLGLF
FNFYKERMPL YEAAKLALQK VQIYLVNEVQ NVYQSQNVDI SDKHIEVIVR QMTSKVKVED
GGDTTLLPGE LVELQQIENI NEAMTLTKGL PARYSPVLLG ITKSSLNTDS FISAASFQET
TRVLTEAAIE GKADWLRGLK ENVIIGRLIP AGTGFNSYND ISKSFVLEKK NQMPDSYEQN
QNEEQNIEDI ILDDNIARNY ALIENPINNF DTIEQNINQS KDI
