RPOC2_SACOF
ID RPOC2_SACOF Reviewed; 1534 AA.
AC Q6ENX1;
DT 21-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 16-AUG-2004, sequence version 1.
DT 03-AUG-2022, entry version 70.
DE RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324};
OS Saccharum officinarum (Sugarcane).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC Panicoideae; Andropogonodae; Andropogoneae; Saccharinae; Saccharum;
OC Saccharum officinarum complex.
OX NCBI_TaxID=4547;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15449542; DOI=10.1093/dnares/11.2.93;
RA Asano T., Tsudzuki T., Takahashi S., Shimada H., Kadowaki K.;
RT "Complete nucleotide sequence of the sugarcane (Saccharum officinarum)
RT chloroplast genome: a comparative analysis of four monocot chloroplast
RT genomes.";
RL DNA Res. 11:93-99(2004).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC Rule:MF_01324}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR EMBL; AP006714; BAD27285.1; -; Genomic_DNA.
DR AlphaFoldDB; Q6ENX1; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Metal-binding;
KW Nucleotidyltransferase; Plastid; Transcription; Transferase; Zinc.
FT CHAIN 1..1534
FT /note="DNA-directed RNA polymerase subunit beta''"
FT /id="PRO_0000067946"
FT REGION 644..698
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 719..800
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 644..666
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 676..694
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 719..745
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 746..789
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 220
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 296
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 303
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 306
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ SEQUENCE 1534 AA; 177035 MW; EB90786F4A493E5C CRC64;
MAERANLVFH NKEIDGTAIK RLISRLIDHF GMGYTSHILD QIKTLGFHQA TTTSISLGIE
DLLTIPSKGW LVQDAEQQSF LLEKHYYYGA VHAVEKLRQS VEIWYATSEY LKQEMNSNFR
ITDPSNPVYL MSFSGARGNA SQVHQLVGMR GLMADPQGQM IDLPIQSNLR EGLSLTEYII
SCYGARKGVV DTAVRTADAG YLTRRLVEVV QHIIVRRRDC GTIQGISVSP QNGMTEKLFV
QTLIGRVLAD DIYIGSRCIA SRNQDIGIGL VNRFITAFRA QPFRAQPIYI RTPFTCRSTS
WICQLCYGRS PTHGDLVELG EAVGIIAGQS IGEPGTQLTL RTFHTGGVFT GGTADLIRSP
SNGKIQFNED LVHPTRTRHG QPAFLCYIDL HVTIQSQDIL HSVNIPLKSL ILVQNDQYVE
SEQVIAEIRA GMSTLHFKEK VQKHIYSESD GEMHWSTDVY HAPEYQYGNL RRLPKTSHLW
ILSVSMCRSS IASFSLHKDQ DQMNTYSFSV DGRYIFDFSM ANDQVSHRLL DTFGKKDREI
LDYLTPDRIV SNGHWNCFYP SILQDNSDLL AKKRRNRFVV PLQYHQEQEK ERISCLGISM
EIPFMGVLRR NTIFAYFDDP RYRKDKRGSG IVKFRYRTLE EEYRTQEEEY RTREEEYRTR
EEEYRTREED SEDEYESPEN KYRTREGEGE YEILEDEYRT LEDEYETLED EYGILEDEYR
TLEKDSEEEY GSLENKYRTR EGEGEYEILE EDSEEEYGSS EDGSEKEYGT LEEDSEEDSE
EDSEDEYGSP EENSILKKEG FIEHRGTKEF SLKYQKEVDR FFFILQELHI LPRSSSLKVL
DNSIIGVDTQ LTKNTRSRLG GLVRVKRKKS HTELKIFSGD IHFPEEADKI LGGSLIPPER
EKKDSKESKK RKNWVYVQRK KILKSKEKYF VSVRPAVAYE MDEGRNLATL FPQDLLQEED
NLQLRLVNFI SHENSKLTQR IYHTNSQFVR TCLVVNWEQE EKEGARASLV EVRTNDLIRD
FLRIELVKST ISYTRRRYDR TSVGLIPNNR LDRNNTNSFY SKAKIQSLSQ HQEVIGTLLN
RNKEYPSLMI LLASNCSRIG LFKNSKYPNA VKESNPRIPI RDIFGLLGVI VPSISNFSSS
YYLLTHNQIL LKKYLFLDNL KQTFQVLQGL KYSLIDENKR ISNFDSNIML EPFHLNWHFL
HHDSWEETLA IIHLGQFICE NLCLFKSHIK KSGQIFIVNM DSFVLRAAKP YLATIGATVH
GHYGKILYKG DRLVTFIYEK SRSSDITQGL PKVEQIFEAR SIDSLSPNLE RRIEDWNERI
PRILGVPWGF LIGAELTIAQ SRISLVNKIQ KVYRSQGVQI HNRHIEIIIR QVTSKVRVSE
DGMSNVFLPG ELIGLLRAER AGRALDESIY YRAILLGITR ASLNTQSFIS EASFQETARV
LAKAALRGRI DWLKGLKENV VLGGIIPVGT GFQKFVHRSP QDKNLYFEIQ KKNLFASEMR
DILFLHTELV SSDSDVTNNF YETSETPFTP IYTI
