RPOC2_SILLA
ID RPOC2_SILLA Reviewed; 1373 AA.
AC Q589B8;
DT 21-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 56.
DE RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324};
OS Silene latifolia (White campion) (Bladder campion).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC Caryophyllales; Caryophyllaceae; Sileneae; Silene;
OC Silene subgen. Behenantha; Silene sect. Melandrium.
OX NCBI_TaxID=37657;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Kejnovsky E., Kubat Z., Hobza R., Lengerova M., Sato S., Tabata S.,
RA Fukui K., Matsunaga S., Vyskot B.;
RT "A partial chloroplast genome of Silene latifolia.";
RL Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC Rule:MF_01324}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR EMBL; AB189069; BAD93459.1; -; Genomic_DNA.
DR AlphaFoldDB; Q589B8; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Metal-binding;
KW Nucleotidyltransferase; Plastid; Transcription; Transferase; Zinc.
FT CHAIN 1..1373
FT /note="DNA-directed RNA polymerase subunit beta''"
FT /id="PRO_0000067947"
FT BINDING 220
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 291
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 298
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 301
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ SEQUENCE 1373 AA; 156521 MW; 1DA4F2539501ED8C CRC64;
MAERANLVFH NKSIDGTAMK RLISRLIDHF GMAYTAHILD QVKTLGFQQA TATSISLGID
DLLTIPSKGW LVQDAEQQSL ILEKHHHYGN VHAVEKLRQS IEIWYSTSEY LRQEMNPNFR
MTDPYNPVHI MSFSGARGNV SQVHQLVGMR GLMSDPQGQM IDLPIQSNLR EGLSLTEYII
SCYGARKGVV DTAVRTSDAG YLTRRLVEVV QHIVVRRTDC GTIRGISVSP RNGRMPERIF
TQILIGRVLA DDIYLGSRCI ATRNQDIGVG LVNRFITFRT QPIAIRTPFT CRSTSWICRL
CYGRSPTHGD LVELGEAVGI IAGQSIGEPG TQLTLRTFHT GGVFTGGTAE HVRAPYNGKI
KFNEGLVHPT RTRHGHPAFL CYIDLYVTIE SEDSLHNVNI PPKSFLLVQN DQYVESEQVI
AEIRAGASTL NFKEKVRKHI YSDSEGEMHW STDVYHAPEF TYGNVHLLPK ASRLWILSGR
PYRSSVVSFS LHKDQDQTTG NSLSFEQKIY NLSMTNDQVQ NKFLDPYIKK SSKKEDRSPN
YSELNGMSHC NHIYPAKNSD LLAKRRKNRL IIPFQFQLSQ EREKELMSLS NGISIEIPIN
GIFRRNSIFA YFNDPRYRTK SSGITKYETI ETHSSVKKED LIEYRGVKEF RPKYQMKVDR
FFFISQEVHI LPGSSSIMVR NNSIIGVDTQ ITLNTRSRVG GVVRVERKKK KIELKIFSGD
IYFPGETDKI SRHSGILIPP GKTNYKESKN IKNWLYVQRI TPTKKKYFVL VRPVVTYEIT
DGINLATLLP QDLLQERGNV QLRVFNYILY GNGKVTRGIY DTSIQLVRTC LVLNWNQDKK
DSSIEEARAS FVEVRTNGII RYFLKIGLMN RALSYIGKRN NPPLFSDDGL EYTNMNPFFS
IYSKPKLQQA FNPNQGTVRM LVGKNKECQS FIILSSSNCF RMGPFTLNGV KYPKESIKKD
RLILIKNSFG PLGTVLNFVN FFFFYHLITH NQILVNNYLQ LDNLKQTCQV LKYQYYLMDE
NGIIYNPDFC SNIILNPFNL HWDFLHYNFC DETSTKMRLG QFICENICIK KNGLHLKPGQ
IIIVQFDSVV IRSAKPCLAT PGATVHGHYG EIIYEGDTLV TFIYEKSRSG DITQGLPKVE
QVLEVRSVES ISMNLEKRIN GWNERIKKIL GIPWGFLVGA ELTIAQSRIS LVNKIQKVYR
SQGVQIHDRH IEIIVRQITS KVLVSEDGMS NIFLPGELIG LFRAERTGRA LEEAICYRTI
LLGITRASLN TQSFISEASF QETARVLAKA ALRGRIDWLK GLKENVVLGG MIPVGTGFKG
FVHRSNQHKS ISIPLKIKNK NRLEGEMRDI LFYHRELLDS CFLKNLGDRS KQQ
