RPOC2_SINAL
ID RPOC2_SINAL Reviewed; 1384 AA.
AC Q9THV5;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 84.
DE RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324};
OS Sinapis alba (White mustard) (Brassica hirta).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Sinapis.
OX NCBI_TaxID=3728;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 6-20, AND
RP CHARACTERIZATION.
RC STRAIN=cv. Albatros; TISSUE=Cotyledon;
RX PubMed=10601874; DOI=10.1046/j.1432-1327.2000.00991.x;
RA Pfannschmidt T., Ogrzewalla K., Baginsky S., Sickmann A., Meyer H.E.,
RA Link G.;
RT "The multisubunit chloroplast RNA polymerase A from mustard (Sinapis alba
RT L.). Integration of a prokaryotic core into a larger complex with
RT organelle-specific functions.";
RL Eur. J. Biochem. 267:253-261(2000).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC Rule:MF_01324}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR EMBL; AJ243754; CAB48415.2; -; Genomic_DNA.
DR AlphaFoldDB; Q9THV5; -.
DR SMR; Q9THV5; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Direct protein sequencing; DNA-directed RNA polymerase;
KW Metal-binding; Nucleotidyltransferase; Plastid; Transcription; Transferase;
KW Zinc.
FT CHAIN 1..1384
FT /note="DNA-directed RNA polymerase subunit beta''"
FT /id="PRO_0000067948"
FT BINDING 224
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 297
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 304
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 307
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ SEQUENCE 1384 AA; 157742 MW; 9A7B4D02053F4A80 CRC64;
MEVLMAERAN LVFHNKVIDG TAIKRLISRL IDHFGMAYTS HILDQVKTLG FQQATATSIS
LGIDDLLTIP SKGWLVQDAE QQSLILEKHH HYGNVHAVEK LRQSIEIWYA TSEYLRQEMN
PNFRMTDPFN PVHMMSFSGA RGNASQVHQL VGMRGLMSDP QGQMIDLPIQ SNLREGLSLT
EYIISCYGAR KGVVDTAVRQ SDAGYLTRRL VEVVQHIVVR RTDCGTIRGI SVSPRNKSRM
MSERIFIQTL IGRVLADDIY IGSRCVAFRN QDLGIGLVNR FITFGTQPIS IRTPFTCRST
SWICRLCYGR SPTHGDLVEL GEAVGIIAGQ SIGEPGTQLT LRTFHTGGVF TGGTAEHVRA
PYNGKIKFNE DLVHSTRTRH GHPAFLCYID LSVIIESEDI IHSVTIPPKS FLLVQNDQYV
ESEQVIAEIR EGTYTFQFKE RVRKYIYSDS EGGMHWSTDV SHAPEFTYSN VHLLPKTSHL
WILSGGSCGS SLILFSIHKD QDQMNIPFLS VERKSISSLS VNNDQVSQKF FSSDFSDKKI
LIPNYSELNG IVGTSHYNFI YSAIFHENSD LLAKRRRNRF LIPFQSIQEQ EQEKEFIPHS
GISVEIPING IFRRNSIFAF FDDPRYRRKS SGILKYGTLK ADSIIQKEDM IEYRGVQKFK
TKYEMKVDRF FFIPEEVHIL PESSAIMVEN YSIIGVDTRI TLNIRSQVGG LIRVERKKKR
IELKIFSGDI HFPDKTDKIS RHSGILIPPG RGKTNSKESK IVKNWIYVQR ITPTKKKFFV
LVRPVATYEI ADSINLATLF PKDLFREKDN IQLRVFNYIL YGNGKPTRGI SDTSIQLVRT
CLVLNWDQDN KNSSLEEVRA FFVEVNTKGL IRDFIRIGLV KSHISYIRKR NNPSSEKKEG
SDHSMNPFYS ISPKAGILHQ SLRQNHGTIR MFLNRNRNPI LLILSSSNCF RIGPFNHVKY
HNVINQSIKK KTSNYIKNCR APYGTAIQIL IFIHFAFTKL INQISCIKYL HLQTSNVFFP
VIHSYLIDEN GRIFNLDPYS NLVLNPFKLN WYFLHQNYNN NYCEETSTII SLGQFFCENV
CIAKKEPYLK SGQVLIVQRD SVVIRSAKPY LATPGAKVHG HYREILYEGD TLVTFIYEKS
RSGDITQGLP KVEQVLEVRS IDSISLNLEK RIKGWNRCIT RILGIPWGFL IGAELTIVQS
RISLVNKIKQ VYRSQGVQIH NRHIEIIVRQ ITSKVLVSEE GMSNVFLPGE LIGLLRAERT
GRALEEAICY RAVLLGITRA SLNTQSFISE ASFQETARVL AKAALRGRID WLKGLKENVV
LGGVIPPGPG SQRIGALSTA TYQHSFGKNK NYLIRGRYER YFILPQGILC LFYQIQNLPF
LELH
