ID   RPOC2_SOLBU             Reviewed;        1392 AA.
AC   Q2MIJ7;
DT   06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT   07-FEB-2006, sequence version 1.
DT   03-AUG-2022, entry version 50.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE   AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE            Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN   Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324};
OS   Solanum bulbocastanum (Wild potato).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum.
OX   NCBI_TaxID=147425;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. PT29;
RX   PubMed=16575560; DOI=10.1007/s00122-006-0254-x;
RA   Daniell H., Lee S.-B., Grevich J., Saski C., Quesada-Vargas T., Guda C.,
RA   Tomkins J., Jansen R.K.;
RT   "Complete chloroplast genome sequences of Solanum bulbocastanum, Solanum
RT   lycopersicum and comparative analyses with other Solanaceae genomes.";
RL   Theor. Appl. Genet. 112:1503-1518(2006).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01324}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC       Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01324}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC       Rule:MF_01324}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR   EMBL; DQ347958; ABC56203.1; -; Genomic_DNA.
DR   RefSeq; YP_538838.1; NC_007943.1.
DR   AlphaFoldDB; Q2MIJ7; -.
DR   PRIDE; Q2MIJ7; -.
DR   GeneID; 3989541; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.132.30; -; 1.
DR   Gene3D; 1.10.274.100; -; 1.
DR   HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR   InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR   InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR   InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR   InterPro; IPR007083; RNA_pol_Rpb1_4.
DR   InterPro; IPR007081; RNA_pol_Rpb1_5.
DR   InterPro; IPR038120; Rpb1_funnel_sf.
DR   PANTHER; PTHR19376; PTHR19376; 1.
DR   Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR   Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR   TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE   3: Inferred from homology;
KW   Chloroplast; DNA-directed RNA polymerase; Metal-binding;
KW   Nucleotidyltransferase; Plastid; Transcription; Transferase; Zinc.
FT   CHAIN           1..1392
FT                   /note="DNA-directed RNA polymerase subunit beta''"
FT                   /id="PRO_0000277201"
FT   BINDING         224
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT   BINDING         295
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT   BINDING         302
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT   BINDING         305
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ   SEQUENCE   1392 AA;  157124 MW;  71368FEEE841B8BF CRC64;
     MEVLMAERAN LVFHNKAIDG TAMKRLISRL IEHFGMAYTS HILDQVKTLG FQQATATSIS
     LGIDDLLTIP SKGWLVQDAE QQSLILEKHH HYGNVHAVEK LRQSIEIWYA TSEYLRQEMN
     PNFRMTDPFN PVHIMSFSGA RGNASQVHQL VGMRGLMSDP QGQMIDLPIQ SNLREGLSLT
     EYIISCYGAR KGVVDTAVRT SDAGYLTRRL VEVVQHIVVR RTDCGTARGI SVSPRNGIMP
     ERIFSQTLIG RVLADDIYMG SRCIATRNQA IGIGLVNRFI TFRAQPISIR TPFTCRSTSW
     ICRLCYGRSP THGDLVELGE AVGIIAGQSI GEPGTQLTLR TFHTGGVFTG GTAEHVRAPS
     NGKIKFNEDL VHPTRTRHGH PAFLCSIDLY VTIESEDILH NVNIPPKSLL LVQNDQYVES
     EQVIAEIRAG ISTLNFKEKV RKHIYSDSDG EMHWSTDVYH APEFTYGNVH LLPKTSHLWI
     LLGGPCRSSL VYLSIHKDQD QMNAHSLSGK PRYTSNLSVT NDQARQKLFS SDFYGQKEDR
     IPDYSDLNRI ICTGQYNLVY SPILHGNSDL LSKRRRNKFI IPLHSIQELE NELMPCSGIS
     IEIPVNGIFR RNSILAYFDD PRYRRKSSGI IKYGTIETHS VIKKEDLIEY RGVKEFRPKY
     QMKVDRFFFI PEEVHILPGS SSIMVRNNSI VGVDTQITLN LRSRVGGLVR VERKKKRIEL
     KIFSGDIHFP GETDKISRHT GVLIPPGTGK RNSKEYKKVK NWIYVQRITP SKKRFFVLVR
     PVVTYEITDG INLGTLFPPD PLQERDNVQL RIVNYILYGN GKPIRGISDT SIQLVRTCLV
     LNWNQDKKSS SCEEARASFV EIRTNGLIRH FLRINLVKSP ISYIGKRNDP SGSGLLSDNG
     SDCTNINPFS AIYSYSKAKI QQSLNQPQGT IHTLLNRNKE CQSLIILSAA NCSRMEPFKD
     VKYHSVIKES IKKDPLIPIR NSLGPLGTCL PIENFYSSYH LITHNQILVT KYLQLDNLKQ
     TFQVIKLKYY LMDENGKIFN PDPCRNIILN PFNLNWSFLH HNYCAETSKI ISLGQFICEN
     VCIAKNGPPL KSGQVILVQV DSIVIRSAKP YLATPGATVH GHYGETLYEG DTLVTFIYEK
     SRSGDITQGL PKVEQVLEVR SIDSISMNLE KRVESWNKCI PRILGIPWGF LIGAELTIAQ
     SRISLVNKIQ QVYRSQGVQI HNRHIEIIVR QITSKVLISE DGMSNVFSPG ELIGLLRAER
     MGRALEEAIC YRVVLLGITR ASLNTQSFIS EASFQETARV LAKAALRGRI DWLKGLKENV
     VLGGVIPVGT GFKGLVHPSK QHNNIPLETK KTNLFEGEMR DILFHHRKLF DSCLSKKFHD
     IPEQSFIGFN DS