RPOC2_STAPU
ID RPOC2_STAPU Reviewed; 1250 AA.
AC Q32RY2;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 06-DEC-2005, sequence version 1.
DT 03-AUG-2022, entry version 56.
DE RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324};
OS Staurastrum punctulatum (Green alga) (Cosmoastrum punctulatum).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Zygnemophyceae; Zygnematophycidae;
OC Desmidiales; Desmidiaceae; Staurastrum.
OX NCBI_TaxID=102822;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16236178; DOI=10.1186/1741-7007-3-22;
RA Turmel M., Otis C., Lemieux C.;
RT "The complete chloroplast DNA sequences of the charophycean green algae
RT Staurastrum and Zygnema reveal that the chloroplast genome underwent
RT extensive changes during the evolution of the Zygnematales.";
RL BMC Biol. 3:22-22(2005).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC Rule:MF_01324}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR EMBL; AY958085; AAX45749.1; -; Genomic_DNA.
DR RefSeq; YP_636394.1; NC_008116.1.
DR AlphaFoldDB; Q32RY2; -.
DR PRIDE; Q32RY2; -.
DR GeneID; 4108611; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 4.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 1.
DR TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Metal-binding;
KW Nucleotidyltransferase; Plastid; Transcription; Transferase; Zinc.
FT CHAIN 1..1250
FT /note="DNA-directed RNA polymerase subunit beta''"
FT /id="PRO_0000277203"
FT BINDING 224
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 314
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 321
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 324
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ SEQUENCE 1250 AA; 141907 MW; 78042AFAC2C940DC CRC64;
MARLTAEFGD RIYYNQVMDR GAMRQLIGRL IIYLGSNCTS QILDQLKTLG FRHATQAGIS
LGVDDLITTP SKTWLIRDAE YQAYTSEEQY RRGSIHAVEK LRHVVETWHT TSEYLKREMT
SHFRITDPLN PVHIMSFSGA RGNVSQVHQL VGMRGLMANP QGNIIDLPIQ SNLREGLSLT
EYIISCYGAR KGVVDTAVRT ADAGYLTRRL VEVVQHVVIR NPDCETNQGI RLHSIRDRKT
TRDAGGNSLL SLQERLIGRV LARNVFLGKR CIAIKNQDLA PDLVNSLVNF PKALLSPGSE
GGHCEILVRS PLTCKSMPRA CQKCYGWDLS CGNLVEIGAA IGIVAGQSIG EPGTQLTLRT
FHTGGVFTGG VAEHVRAPFN GIVHFSISSS THKTKTIQPT RNRHGRPAWT CPEAFSVIIE
DRIGKKKVLN VPQYSLLLVK NHQYVQSRQI IAEVRASIAP LKEKIEKNIY SHLQGEVFYN
PIGLRASCLK QRHFLHFLCS ASRMNLWIEF ASIRSYLSPL VEKTSYIWIF SGRLNKFFNA
TLNLSFFYQT QDYIQRDLPI GIQNHVFNYS KVFLLSIYNR QRSVTFSVSR RDIRLGRKKK
FTDYIPLSRI STDKIFSKHT GSIHTISEQN EWILTLSFVD QFSKKMFSSV QTPGTSKVSE
KSEEQIIDFQ EQLILNYPLS KTTIFLKEPS ILAKNYSIAE KDSCIGLLGE FFIFLNELSI
VSVRTFISTS QINLFLKKNV FSLLCITHGN TFKGKEKIFF INDSKNIYNI KNWLISVNFL
IWCHFSNRIL NKNQTLATIF LSNRSLRNEK DLQVKEFKIG QSICPSRDLG EKIFEPFTHS
ILPESGHLLS LNEGNLLIRL AKLYLIPTGG IAHTFHKQTI NEGDTLVTLA YERLKASDII
QGLPKAEQLL EARVGNQVVI NLYMRFEILV ARIKEQLRNY IGSLTQMDLM LSQISVNRAS
KALEYSQLET VDQVQKVYLS QGVYISDKHF EVIVRQMSSK IAIVENTDFT AFSPKAVIRT
AFPYDVLGVF FPGEILDISK AQKMNRVLYK PLPCKPVLLG ITQASLNANS FLSEASFERT
ITVLSRSALQ GRMDWLKGLK ENVLLSKMIP AGTGLKQAPV LLSPNGTGTL FSNNEQNIFQ
KKNVTSLSKS KGQKVQIRKI FSFQKSPQRL SFLYQETSSS NIRRKIQPTV CLCKPFITKR
NWLHISLKQL TEQSLNYETK KFCLQELTNN FIPRKKENIF YLKKKTIKTT
