ID   RPOC2_STIHE             Reviewed;        2430 AA.
AC   Q06SF2;
DT   04-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT   31-OCT-2006, sequence version 1.
DT   03-AUG-2022, entry version 52.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE   AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE   AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE            Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN   Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324};
OS   Stigeoclonium helveticum (Green alga).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC   OCC clade; Chaetophorales; Chaetophoraceae; Stigeoclonium.
OX   NCBI_TaxID=55999;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=UTEX 441;
RX   PubMed=16944205; DOI=10.1007/s00438-006-0156-2;
RA   Belanger A.-S., Brouard J.-S., Charlebois P., Otis C., Lemieux C.,
RA   Turmel M.;
RT   "Distinctive architecture of the chloroplast genome in the chlorophycean
RT   green alga Stigeoclonium helveticum.";
RL   Mol. Genet. Genomics 276:464-477(2006).
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01324}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC       Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC   -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC       composed of four subunits: alpha, beta, beta', and beta''. When a
CC       (nuclear-encoded) sigma factor is associated with the core the
CC       holoenzyme is formed, which can initiate transcription.
CC       {ECO:0000255|HAMAP-Rule:MF_01324}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC       Rule:MF_01324}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR   EMBL; DQ630521; ABF60191.1; -; Genomic_DNA.
DR   RefSeq; YP_764414.2; NC_008372.1.
DR   AlphaFoldDB; Q06SF2; -.
DR   GeneID; 4308371; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.132.30; -; 1.
DR   Gene3D; 1.10.274.100; -; 1.
DR   HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR   InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR   InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR   InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR   InterPro; IPR007083; RNA_pol_Rpb1_4.
DR   InterPro; IPR007081; RNA_pol_Rpb1_5.
DR   InterPro; IPR038120; Rpb1_funnel_sf.
DR   PANTHER; PTHR19376; PTHR19376; 5.
DR   Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR   Pfam; PF04998; RNA_pol_Rpb1_5; 2.
PE   3: Inferred from homology;
KW   Chloroplast; DNA-directed RNA polymerase; Metal-binding;
KW   Nucleotidyltransferase; Plastid; Transcription; Transferase; Zinc.
FT   CHAIN           1..2430
FT                   /note="DNA-directed RNA polymerase subunit beta''"
FT                   /id="PRO_0000353585"
FT   BINDING         336
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT   BINDING         455
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT   BINDING         462
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT   BINDING         465
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ   SEQUENCE   2430 AA;  280075 MW;  38C74C37BAD1B380 CRC64;
     MYLYKSIKSY KMQGKSKRKK EAFPTSKACG KLGKNRIFPL EEKRSGKKER PLALSFQASF
     FSPFFLFANC TRKTKTLKQN SLSLSSSYLN IFSDFSLYSL SPKKKTSLTD NKIVSFATKD
     FPQVFWNLSF NKGSLKNFVF WCFVNYGQRE TIKILEKLQI IGFGYATKAG ISLSIDDLKI
     PPTKAILLAD ADKSIEAGFL SYKKSQLTGL ERFQRIIETW HKTSESLKDE MITNFRQTDS
     LNPVYMMAFS GARGNVSQVR QLVAMRGLMS DPQGKILDFP IRSNFREGLT LTEYVISCYG
     ARKGVVDTAL RTANAGYLTR RLVDVAQHVI VLNFDCGTKK GFYLTEMKKF NKTLYPLRQR
     LLGRVLAANL YNVNPFHLKK RIEIGEKEKN LSSLPSSKGK MEFFPSFPQA SLAGKASCFP
     EPQIVAFRNQ EISEPLAEKI SKITNKVFIR SPLTCDTPRL VCQLCYGWSL SEGYLVSIGE
     AVGIIAAQSI GEPGTQLTMR TFHTGGVFAG EMFDQLMAPY DGIVQYSSSI PGTLVRTTQG
     KIAFLTKVEG KLSVFEKNNS TFLSNSNNGD NHSLISHTIS HKSFKLPAYT LLFIRNNETI
     SKDQLIAELA FLSSKGKKKG EIAEFIVKSE IEGQLYSGSV NILEKYTDYN DIMTKSIDWG
     SVWILSGKIC QLPVNSSFFA LPGDLVDENA ILSQIQWVVP MKSLLDTNSL IKPNLKKEIY
     FDFSLNQTKW FQKRNLENLN TLFNLKFGIE KKNRTKKRVL SQEKKSDFLN PSFSHSSRNY
     LYFSLPSKKT FSYYPFAYFS KIKEKNGGRE TKSSFAMSGW GKKSLSFFPS LASDVPKLLS
     DFYPTSKKRN FKSFEKKELK LSSFSQTIFF NQDKSLLLKM KIKIQTISDF TKVSSKMITS
     RELKKNGMEK GKNGEKENSS LQRTFFSQGA KAPAFPELFS FFSSRLFPIL PFKSSLVSST
     FSHNSQLCTI RRQNKLVLSG NSNQFENFAY LKDKITINLP LIFLCIDNIY YKKIGYFFSL
     FPPESKIPRE KFLPEEEGFS PPSFGRTFKN KNLAKLKDVF FVPKLLEQRN IISTKKKNSF
     KKNNQISNQL LHWFPKSYLT MKGGICVYRL THDFFCQYIN FHSSSFLFEK KRGTLICNVD
     GEMNKPVSSN ILGRICWVNQ DYKKTKLYTL KFFSYSKFIQ QLTDRTKRLQ RQIKREKKIG
     QADFFQTVLS MSQAKEKSSL QRTFFSQGAK SRKKVPPKTL LSGEMPIFSL SPSLCGLKPA
     KRSEKEKIWV KGEKAELFSL SNAFHQPQHS KKHKYSSFSL NFYKNLTSYC LKNKDKSVAN
     CFSEKKKRQF LFFSEPKLSL FPDFLFSKSE KNIFYNANKK KYSFFYLKKT NSSKKSLTNN
     SIMNGFIYKP KDVVSAISLH KKFFLPGQKI IDDLSFDNFL IYVECIPEDN LSSLSETLYK
     SRFDKKICFN LKKLITNKMP RFIKIPTLLS TIPFAFLKSE FKEQNEKIAQ RKADRQKNNK
     KSEAFFFKSN TLVSDFDSPF LSHLVVLITK VIEYPLYKPN HYKKALYNRQ KTSEQSFFHY
     SCKVKSKMLN KIFKNNYHSN VLNKQTSSLR LLDKFPNTDL VLALKCKGIQ KDLMENFLKN
     PLNLNLYVKL GKQKSFGKEK DKTFSVSQPF FKSSAFKKEK KSNSFSQAKA FDFNLTSKKS
     RKKANKLVEN LANGKKSRAF PASKACGKLG KNYIFPLEER KNGKKERAKA SSFPGLLSFS
     HLAFPIFFRK SFSKFFCMKF FSKSSIQLLE LVLVGVQKNS VNNKIIGITV FNFFQKNKLK
     EEEKKRQEEK EIFRSLSLSP LFLSHLTRKK NSDFFSVSKS EALEKKKLQL LFFSHAAFPL
     KEISKNYSSK ASFLSFFHFL DKKIKKQTRR QANFVVSAFE KSDFSFDPNI NRKKKYFSNK
     AFNYENIKIM NNNISQSILQ PNFYFNYYQK YPFGYYLINN INVFAPSSFS FSSLFSRNTA
     NFKNYFLFYQ LLSMFNRPNF QLHKSMEENL LQVYEQSTSY FSLISCFFQQ PCFDAFFTQQ
     VSFGFNEVKN VNHYSKNLTF SKKIGKRREF YLSPFLRSKI YFKKNSFSEQ GEIALTQYLS
     PFMGEILNHE NYYWSQNTQK NRYLLLTKKD QISFLSTNYN TQKESINNSS FFILKNKKNT
     PNLGEFLTKG DCCFISKSQN SIISSESGLI VHSNKSKITL RKAQSFFLSP NCIFHYSHGD
     LVEKNKSILS LPYEQLKTGD IVQGIPKVEQ LLEARSTFKG KEEEDNLHKL LKYAFELYKT
     KFSLKLSVRK SFSFIQLILV NSVQRIYRSQ GVSISDKHLE VIVKQMTSKV QITSRGDSSF
     FRGEHVDLYI VETWNALHPQ LKKICYKPIL LGISRSSLEV NSFLSAASFQ HTKKVLSRSA
     FKTNIDFLNG LKENVIIGNL ISAGTGNLNN