ID   RPOC2_SYNP2             Reviewed;        1331 AA.
AC   B1XHW1;
DT   04-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT   20-MAY-2008, sequence version 1.
DT   03-AUG-2022, entry version 88.
DE   RecName: Full=DNA-directed RNA polymerase subunit beta' {ECO:0000255|HAMAP-Rule:MF_01324};
DE            Short=RNAP subunit beta' {ECO:0000255|HAMAP-Rule:MF_01324};
DE            EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE   AltName: Full=RNA polymerase subunit beta' {ECO:0000255|HAMAP-Rule:MF_01324};
DE   AltName: Full=Transcriptase subunit beta' {ECO:0000255|HAMAP-Rule:MF_01324};
GN   Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324};
GN   OrderedLocusNames=SYNPCC7002_A2043;
OS   Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) (Agmenellum
OS   quadruplicatum).
OC   Bacteria; Cyanobacteria; Synechococcales; Synechococcaceae; Synechococcus;
OC   unclassified Synechococcus.
OX   NCBI_TaxID=32049;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 27264 / PCC 7002 / PR-6;
RA   Li T., Zhao J., Zhao C., Liu Z., Zhao F., Marquardt J., Nomura C.T.,
RA   Persson S., Detter J.C., Richardson P.M., Lanz C., Schuster S.C., Wang J.,
RA   Li S., Huang X., Cai T., Yu Z., Luo J., Zhao J., Bryant D.A.;
RT   "Complete sequence of Synechococcus sp. PCC 7002.";
RL   Submitted (FEB-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC       DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC       {ECO:0000255|HAMAP-Rule:MF_01324}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC       Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC   -!- SUBUNIT: In cyanobacteria the RNAP catalytic core is composed of 2
CC       alpha, 1 beta, 1 beta', 1 gamma and 1 omega subunit. When a sigma
CC       factor is associated with the core the holoenzyme is formed, which can
CC       initiate transcription. {ECO:0000255|HAMAP-Rule:MF_01324}.
CC   -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR   EMBL; CP000951; ACB00030.1; -; Genomic_DNA.
DR   RefSeq; WP_012307652.1; NC_010475.1.
DR   AlphaFoldDB; B1XHW1; -.
DR   SMR; B1XHW1; -.
DR   STRING; 32049.SYNPCC7002_A2043; -.
DR   EnsemblBacteria; ACB00030; ACB00030; SYNPCC7002_A2043.
DR   KEGG; syp:SYNPCC7002_A2043; -.
DR   eggNOG; COG0086; Bacteria.
DR   HOGENOM; CLU_000524_1_0_3; -.
DR   OMA; IEGKSDW; -.
DR   Proteomes; UP000001688; Chromosome.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.132.30; -; 1.
DR   Gene3D; 1.10.274.100; -; 1.
DR   HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR   InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR   InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR   InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR   InterPro; IPR007083; RNA_pol_Rpb1_4.
DR   InterPro; IPR007081; RNA_pol_Rpb1_5.
DR   InterPro; IPR038120; Rpb1_funnel_sf.
DR   PANTHER; PTHR19376; PTHR19376; 4.
DR   Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR   Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR   TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE   3: Inferred from homology;
KW   DNA-directed RNA polymerase; Metal-binding; Nucleotidyltransferase;
KW   Reference proteome; Transcription; Transferase; Zinc.
FT   CHAIN           1..1331
FT                   /note="DNA-directed RNA polymerase subunit beta'"
FT                   /id="PRO_0000353533"
FT   REGION          1236..1257
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1294..1331
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         220
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT   BINDING         293
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT   BINDING         300
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT   BINDING         303
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ   SEQUENCE   1331 AA;  145422 MW;  C9D101BC915BB278 CRC64;
     MTKEKPAVFY NRIIDKGRLK KLMSWAYTSF GSAHCATMAD ELKTLGFRYA TQAGVSISVD
     DLQVPPIKRQ MLDSAEQEIK TTEARYSRGE ITEVERFQKV IDTWNSTSES LKDEVVKNFR
     ETNPLNSVYM MAFSGARGNL SQVRQLVGMR GLMADPQGEI IDLPIKTNFR EGLTVTEYII
     SSYGARKGLV DTALRTADSG YLTRRLVDVS QDVIVREEDC GTARGLKLRA MTDGEREQIS
     LEDRLFGRVL NADVVDPKTG EVIAQRNQDI DADLAKKIAT TVAEVEVRSP LTCEAARSVC
     RKCYGWSLAH GHMVDMGEAV GIIAAQSIGE PGTQLTMRTF HTGGVFTKEA ARTIKASKAG
     TIQFKDGLST RRMRTPHGDE VEQVEVAGTL VLKPSDNGKM VSHALSPGSF VLVAEGASVK
     KGDLLVEVGA GQKTQKSTER ATKDVSSDLA GEVLFDNLIA EEKTDRQGNT TRSAQRSGLM
     WVLAGDVYNL PAGAEPVVEN GTHVNVGDIL AETKLVSLSG GVVRLIPNSR EIEIVTASVL
     LDEAKVLHET GGGSEQYIIE TSKGDQFLLK TAPGTKVQNN ANIAELIDDR YRTTTGGIIK
     YSGVEVAKGT KKQGYEVLKG GTLLWIPEET HEVNKDSSLR IVEDGQYVEA GTEVVKDIFS
     QSAGVAEVIE KNDILREVII KPGELHLTEE AIADKYHEQL IQPGEEVIPG VTIDKLSYGE
     KVISTEGVAL LVRPVEEFQV EDTPVEPSQG SINEQGAGRN IELHAVQRLF FKDGERVKSV
     DGVSLLSTQL IIEIGAIEGE DEDVLANLYA DIELQDDPTD DEVKRLQLVI LESLILRRDS
     DSDPFGGQVQ TRLMVEDGQE IVPGAVVART EIQCKEPGEV RGIRSGQEAI RRLLIVRDSD
     RQTLAIDGKA KVKENTLVVA GTEIAEGVVI EDSAQVLKVS DKEIVLRHAR PYRVSGGAVL
     HIDEGDLVQR GDNLVLLVFE RAKTGDIIQG LPRIEELLEA RKPKEAAVLA RRPGTCQVEY
     LDDETVDVKV IEDDGVISEY PVSLNQSVMV VDGQRVGPAE PLTDGLNNPH EILEIFFDYY
     AESKGIYEAA LIGLRESQRF LVEEVQRVYQ SQGIDISDKH IEVIVRQMTA KVRIDDGGDT
     TMLPGELIEL RQVEQVNEAM SITGGAPARY TPVLLGITKA SLNTDSFISA ASFQETTRVL
     TEAAIEGKSD WLRGLKENVI IGRLIPAGTG FASQNDFVDE GTSRSPNGYS NVVTNDNGAG
     LSSRTYDDLD GSEILDDQTA RAFTEGKSNR KDIISGDELI SDDTPIPSDV QGKAPVIDDD
     AMIDDNWMKD Q