RPOC2_SYNY3
ID RPOC2_SYNY3 Reviewed; 1317 AA.
AC P73334;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=DNA-directed RNA polymerase subunit beta' {ECO:0000255|HAMAP-Rule:MF_01324};
DE Short=RNAP subunit beta' {ECO:0000255|HAMAP-Rule:MF_01324};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=RNA polymerase subunit beta' {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=Transcriptase subunit beta' {ECO:0000255|HAMAP-Rule:MF_01324};
GN Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324}; OrderedLocusNames=sll1789;
OS Synechocystis sp. (strain PCC 6803 / Kazusa).
OC Bacteria; Cyanobacteria; Synechococcales; Merismopediaceae; Synechocystis;
OC unclassified Synechocystis.
OX NCBI_TaxID=1111708;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PCC 6803 / Kazusa;
RX PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T.,
RA Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.,
RA Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence analysis of the genome of the unicellular cyanobacterium
RT Synechocystis sp. strain PCC6803. II. Sequence determination of the entire
RT genome and assignment of potential protein-coding regions.";
RL DNA Res. 3:109-136(1996).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- SUBUNIT: In cyanobacteria the RNAP catalytic core is composed of 2
CC alpha, 1 beta, 1 beta', 1 gamma and 1 omega subunit. When a sigma
CC factor is associated with the core the holoenzyme is formed, which can
CC initiate transcription. {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR EMBL; BA000022; BAA17364.1; -; Genomic_DNA.
DR PIR; S77517; S77517.
DR AlphaFoldDB; P73334; -.
DR SMR; P73334; -.
DR IntAct; P73334; 2.
DR STRING; 1148.1652442; -.
DR PaxDb; P73334; -.
DR EnsemblBacteria; BAA17364; BAA17364; BAA17364.
DR KEGG; syn:sll1789; -.
DR eggNOG; COG0086; Bacteria.
DR InParanoid; P73334; -.
DR OMA; IEGKSDW; -.
DR Proteomes; UP000001425; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR007066; RNA_pol_Rpb1_3.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 4.
DR Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase; Metal-binding; Nucleotidyltransferase;
KW Reference proteome; Transcription; Transferase; Zinc.
FT CHAIN 1..1317
FT /note="DNA-directed RNA polymerase subunit beta'"
FT /id="PRO_0000067910"
FT REGION 1279..1317
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1288..1317
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 214
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 286
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 293
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 296
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ SEQUENCE 1317 AA; 144777 MW; 27B6970469E7E551 CRC64;
MTFYNYTIDK GRLKKLIALA YRRYGSARCS QLADELKELG FRFATKAGVS ISVDDLTIPP
EKKQMLEAAE KEIRTTEERY ARGEITEVER FQKVIDTWNG TSEELKDQVV VNFRKTDPLN
SVYMMAFSGA RGNMSQVRQL VGMRGLMADP QGEIIDLPIK TNFREGLTVT EYVISSYGAR
KGLVDTALRT ADSGYLTRRL VDVSQDVIVR EQDCGTERSL RVTAMTDGDQ VKISLADRLF
GRLLAKDVVG PDGEIIAKRN DEIDEALANR IAAVTDEVYV RSPLTCEAAR SVCQNCYGWS
LAHGHKVDLG EAVGIIAAQS IGEPGTQLTM RTFHTGGVFT GEVARQEKAP EDGTVKWGKG
LSTRKVRTRH GEDAEQVEIA GDLIWKGEGK KAATQTYSLT PGSLLFVQDG QTVTAGQLMT
EISLSKTQRS TERATKDVAG DLAGEVLFDR LVPEEKTDRQ GNTTRIAQRG GLVWILSGEV
YNLPPGAEPV VKNDEQVEVG SIMAETKLVT NDGGVVRLVS NREIEIITAS VLLDQAQVKL
ESSGGREQYV IYTADKQRFL LKAAPGTKVQ NHSIVAELID DRYRTTTGGM IRYAGVEVAK
GGRKQGYEVT KGGTLLWIPE ETHEINKDIS LLIVEDGQYV EAGTEVVKDI FCQSSGIVEV
VQKNDILREI IIKPGDFYQD VDPGSVKIES GQLLQPGQDV FPGVTVSTLS QAEWIESPEG
NGLLLRPVEE YKVFDEPAAP SQGSQNEEGG RQIELRSVQR LFYKDGDRVK SVEGAPLLST
QLVLEIYGSG NEGISHLSAD IELQDDEEED CQRLQLVILE SLVLRRDQES DPLGGASKTR
LLVQDGDQIP PGAVVARTEI QCKEAGTVRG IKEGQESIRR VLLERAADRL VVDLPSAPEV
KPGQLLVAGQ ELVPGVKLEE SGKVLEINGK GDNYQLVLRR ARPYRVSPGA VLHIEDGDLV
QRGDNLVLLV FERAKTGDIV QGLPRIEELL EARKPKEACV LARAPGVCQV EYLEDESVDI
KVVEDDGTVS EYPLLPGQNA MVTDGQRIDV GHALTDGYNN PHEILDVFFS YYVDKDGCYQ
AALRGLQAAQ KFLVNEVQTV YQSQGVDISD KHIEVIVRQM TAKVRIDDGG DTTMLPGELV
ELRQVEQVNE AMGITGSAPA RYTPVLLGIT KASLNTDSFI SAASFQETTR VLTEAAIEGK
SDWLRGLKEN VIIGRLIPAG TGFSSHEEVL GLIETQDDIQ GYMIEPIELP TTKKKASATK
VKTKKVEADD DLLDDTRARA YAGTQLSQDD EEFEETYDTD EDDFDMDDDD DFGDDED
