RPOC2_TETOB
ID RPOC2_TETOB Reviewed; 2552 AA.
AC Q1KVX8;
DT 04-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2006, sequence version 1.
DT 03-AUG-2022, entry version 54.
DE RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324};
OS Tetradesmus obliquus (Green alga) (Acutodesmus obliquus).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Sphaeropleales; Scenedesmaceae; Tetradesmus.
OX NCBI_TaxID=3088;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=UTEX 393;
RX PubMed=16638149; DOI=10.1186/1471-2148-6-37;
RA de Cambiaire J.-C., Otis C., Lemieux C., Turmel M.;
RT "The complete chloroplast genome sequence of the chlorophycean green alga
RT Scenedesmus obliquus reveals a compact gene organization and a biased
RT distribution of genes on the two DNA strands.";
RL BMC Evol. Biol. 6:37-37(2006).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC Rule:MF_01324}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR EMBL; DQ396875; ABD48228.1; -; Genomic_DNA.
DR RefSeq; YP_635946.2; NC_008101.1.
DR AlphaFoldDB; Q1KVX8; -.
DR PRIDE; Q1KVX8; -.
DR GeneID; 4099813; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 4.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 2.
DR TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Metal-binding;
KW Nucleotidyltransferase; Plastid; Transcription; Transferase; Zinc.
FT CHAIN 1..2552
FT /note="DNA-directed RNA polymerase subunit beta''"
FT /id="PRO_0000353584"
FT BINDING 335
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 414
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 421
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 424
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ SEQUENCE 2552 AA; 299524 MW; 8F6AB847E23FD46A CRC64;
MNKFKNKKIL NTVLKKEFSK NTVSFQQKFV CSVFSQKNSF GIIQTNKQKF LEFEPKSTKQ
FFGSKNSYDQ KNQMSSSGFI FWKQNFKTKV AFQKENTIFL SALKNQKHFP LFVGKENSLL
EPIFFNFAFD KNRLKNVVAW FLERYGQYKT IQFLEKLKEF GFGYATKGGL SLGIDDLKIP
NNKISLIAKA ETKVSKDLMD FRNAKITGVE RMQRLIYTWN QTNDMLKQEV IKYFETMDLF
NPIYMMAFSG ARGNMSQVRQ LVGMRGLMSD PQGQIIDFPI QSNFREGLTL TEYLISTYGA
RKGIVDTALR TATAGYLTRR LVDVAQHVIV SQFDCETSRG IFLFDMKEAN KTIYSFQNRL
VGRVLAQDIF STKEINKISD KKLIASRNQE IDSKLAFAIS KVTKKALVRS PLTCETPRLI
CQLCYGWSLS QGKLVSVGEA VGVIAAQSIG EPGTQLTMRT FHTGGVFAGG LTDQILAPFD
GKIQYFHNIP GSCIRISLSE IAFFTKTPGS FSVIRGSKAS ENIHDLSFNP HCDETYVSQF
ENNWKQKNKN SNFSEIYKIP AYAVLFMRNH EFVQKKQVLA QFSTVAKKQL QYGSAEETLY
SILAGEFYLS TSSVPFQKKD QFQKLQLLIE TKSSDSFDLS ALELKNDVLW KVKDWTNLWI
LSGKVLYNSF DSNLFIKNGD FLKKTTALNR ILWKKTKTWN LFGFKKQNDF KKKKKRNMFY
SMALENFKNS KPFPFFVEKS LSSSFILNST FQQNTQIFPI FSFRKNFFSL KKNNSKKRKN
SLFNIWKISS KQRAFQKEIF KKNKKSFKKN NLTWKIFFDF SKNRSIFKFQ LLPFRPFSAN
FQNSYKKQLD KNQKFLKPKK DIIFSGNFQR FFVFSQKQKK FKKLFFFKKK QQQNRENLNC
FIYKRKNPLK QIFFKFSTFS QNSPFFKVYH FAKKFNTSQK FVKRTSFSKN FFSEKNYLLS
KFLLSKKYST KIFYKSEQSS QKEHFDLQKI EKKCFFFKTK ATLKLFSVQN ANKFFPTSFS
SENKNLTFLF STHSLKFPFF NALNFFHSQK IRKTFSQSLS FQKTNSQKII LKKTLLCLNY
EKVRYKKFGY VFSFSDSLTT QNSKNSQLCF RTFTTLKQTV FDENKQANFG KQQTSLAIGN
ENFFGKDIPT FLTYCFPKNY QTKTNGIFTI FVFENFSKKQ KFTQFLCVQE KSSFDFFKIL
KTGKNQNKKD DRKKNNFFVQ PQKNLFLTIS PFACVLQNKS AFFENLFSIQ KTKNVFENFL
RTNSKQKELF THSFLNLKKR KEVDSNNLKF DLFFGLKKLQ TKSTFNRFNS ELYTEEIFWI
PQENYTFSVL NFSNKDQYNF GVKTNQNFKE QEKMKSYVFD IIFQKTPLFY SSNRQGIKKL
FFPVFEGIWT YSTFSNQLKY QQLNSFQKKN FSPKNNKKLQ QKISFYQHKD KKVQSQRKDY
SSFFYNFKKN KKTKSIQKIS LQKKNFSLRF LQWKFLLQNF LKTKIARKNS FLFKQKNRKF
FKFSPKFYFS KKLNKQLKNN ISLSFFKNKQ NKSNFEKSQK IFNKVQQSTF FKKVKIQDTV
GQFVNSNCFT CFPLQKVNQN SNFSSQNFMN QFENTFIPNS KFAVSHNTNI DSSQSNLLKK
SKLDSKQIHL TIQSGWLCIF ENSSFVLNSH QTTANQGQFF AKDLCFEQNK IFKEAILLKR
SRLSSKPQNM LLKQISLNSE FKVVSLFFKE KQQKNKNFSV TSVQKNLYCR FQNNESLDAL
ENFASSFEEN KAFNNTKIFD GQRKRMVQNI NFDKKEFKMN STLSKQMKKL ALFIRPIHYK
LLENPKNYKK FFLTATIQKS SQKNSILYNS SLKIYKKYHS TLLNSQHPQK NFLYHSKNPD
SFVSSAFEKI HSLKKFRKIL ISRMAFFQTF KPSFKKNQSF LDFRLSKKDT LKKSLNFKLY
SKKTVLSNFR NVLNRDFSKH FLHGKDQPQN LNLKQASFQF SQKNLKKNFQ NNSFFSFYPI
QFLPLSISHS CSTNSSFHFQ TSQTSPTLFF NAVNSTIDFS ICEKKAVFLN QRFVFFKKSF
ETVSFWLNKD GLKFLFPEIY DQNTSKNFWK QSFSLFVNKN TIEKNKKNFF SPKNKPSKTP
QIVQNFIFKK NPVFYANTKF LSPFEGELIP MHMNETNWWK KASEISTIQK LNNLFSVVTK
NDLFTVDFSK PNSQNFFSSF LTLSQQNGKN QHSAENEKKY LFQNKQRHLL ELYKFVLNKQ
KIVENDELFG LQKQEEFLSH SFKDNSIFVK KQKTANFEIS SFSTKYENKL YTFKNLKIGY
PKLSKKPILG KFFVYGDCVS NLAIRKPGQI VHISSMKITL RRGQPFLVSP KGILHFANTP
YIQKNIPILT LAYQTVQSGD IVQGIPKVEQ LFEARTTIQG RLFLSSLPIL LKGIFERYKT
MLPLEQAVRQ SFLKIQQLIV DGVQRVYRSQ GVSIVDKHLE VIVRQMTTKV QILHGAQTGF
FPGELVNLDL VERINKFLLV KIRYEPVVLG ITRSSLEVDS FLSASSFQQT TKILALASIS
RKKDFLKGLK ENILVGNLMP SGTGYMVLRK NL
