RPOC2_THAPS
ID RPOC2_THAPS Reviewed; 1446 AA.
AC A0T0Q9;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 1.
DT 03-AUG-2022, entry version 58.
DE RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324};
OS Thalassiosira pseudonana (Marine diatom) (Cyclotella nana).
OG Plastid; Chloroplast.
OC Eukaryota; Sar; Stramenopiles; Ochrophyta; Bacillariophyta;
OC Coscinodiscophyceae; Thalassiosirophycidae; Thalassiosirales;
OC Thalassiosiraceae; Thalassiosira.
OX NCBI_TaxID=35128;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CCMP1335 / NEPCC58 / CCAP 1085/12;
RX PubMed=17252281; DOI=10.1007/s00438-006-0199-4;
RA Oudot-Le Secq M.-P., Grimwood J., Shapiro H., Armbrust E.V., Bowler C.,
RA Green B.R.;
RT "Chloroplast genomes of the diatoms Phaeodactylum tricornutum and
RT Thalassiosira pseudonana: comparison with other plastid genomes of the red
RT lineage.";
RL Mol. Genet. Genomics 277:427-439(2007).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC Rule:MF_01324}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR EMBL; EF067921; ABK20744.1; -; Genomic_DNA.
DR RefSeq; YP_874521.1; NC_008589.1.
DR AlphaFoldDB; A0T0Q9; -.
DR SMR; A0T0Q9; -.
DR STRING; 35128.Thapsdraft2050; -.
DR PRIDE; A0T0Q9; -.
DR GeneID; 4524727; -.
DR eggNOG; ENOG502RUNH; Eukaryota.
DR InParanoid; A0T0Q9; -.
DR OMA; IEGKSDW; -.
DR Proteomes; UP000001449; Chloroplast.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0005666; C:RNA polymerase III complex; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 4.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 1.
DR TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Metal-binding;
KW Nucleotidyltransferase; Plastid; Reference proteome; Transcription;
KW Transferase; Zinc.
FT CHAIN 1..1446
FT /note="DNA-directed RNA polymerase subunit beta''"
FT /id="PRO_0000277204"
FT BINDING 217
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 285
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 292
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 295
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ SEQUENCE 1446 AA; 166689 MW; 980304EF523A79A9 CRC64;
MKDYIYQNTL INKKQLKELL AWSFSKYDSM QASLLADELK YLGFKYATQA GISISIEDLK
VPATKNEMLE KANKDILNAE KICLKGKITD VERFQKIIDT WSIASESLKD NVVSYFKTYD
PLNSVYIMAF SGARGNLSQV RQLVGMRGLM ADPSGEIMRV PIKKNFREGL TITDYLMSGY
GARKGIVDTA LKTANSGYLT RRLIDIAQDI VIREKDCLTS SSFLIDSQTK FDTEQLIGRV
LLKSVYDSKT EKLIAKANTQ LTLDLLNLFN QKQISKFYIR SPLTCNLYHS ICQMCYGWDL
SNQNLVDLGE AVGILAGQSI GEPGTQLTMR TFHTGGIFTS EARQQIISPK NGIIKFSKIL
KTIILRTNRG EDVLLTKNSG SLILIPEKHT DEIIQIELLR NTMLFVKSNQ YIKQSAIIGE
LISTDKQTLT ERKPILSDTA GEIFIPRLKT RMNLITQNRL LWILSGQVYQ APSNSFLNFY
TDHKINKNSY IFRTKLINQY SGYTKVLNQN KNLLEQKIQI TNETYFLENS YVQKILKPIT
NKNYLLNFNN LTYLITLKDS NSKNWKRCKK YKPFATSFNN NFKTLTGGIM YYDQRIKQKF
DSFNTLISYN IAYEISKEDY DKVSKDHYEN YLKKLENKID KNGFIFYQNF LNYQEMESIF
LKFKLKKKIV HNSLIWLSEE TYKLNCDKNI LLVENGNFIA KNFEIIPNII SKTAGIITVS
QKNNMIEEIA IKAGFVYQGK QFEQLDKKVY YPGEIIFDNI KITQPSLCEH INNKSNNQLL
IRPFAIYEIP KEKTVKSLFS DKNNSKSIFT ITNKITYLYK TNQKIKTSNH INLISQNINL
KAKNSVQNNT IIDLSNSFKT KRLNLRISEN LLLKHYIPAH LKYTNLQYCL LVEPTQFIDS
YTTLGYLESL ITNSLEIVKF KSKRANKKQV FLISNKDCIT VRKEQGKNKT ANELIIDNIN
VNQTGKVLID NGQFLTVQKG RPYFFPNCKS DDSKEKLDLQ YKLIGLNNTI SNNTNTFLNY
YDVTKRSLVE RLGPNKKSYG FKVKFSKMFI KKNGKFYSSP IPLFLNNFSI IKEEQKRLNE
GKNEINNFII KNTQLKIKQC LPLLLKSSEL VTNKIKRKTP FNNNLTGLKF LKYPFNKSIG
IHSLTEDYFE QEVNNVYCKN GEFIEKGEVI GLLNFEKEIT GDIVQGLPRI EELLEARKKK
PTNKHLATNQ KKSLLIQKTS IDSSFEFQKL GTTIKENDKI NPHNLLKIYF NYYGIKKQFF
SNKEMFALSY RLTDNYEASY RTFKKIQLLI LNAVQSVYES QGVSIANKHL EVIIKQMTTK
VLITHEGHTP LLPREVVDLY HIQYINKIIE AHNKRPAYYV PLLLGITKAA LNNPSFISAA
SFQETTRVLT KAAIEGRVDW LRGLKENIII GHLIPSGTGY QSYSNCFNQL AQSKTKINLE
KIKIKI
