RPOC2_TRICV
ID RPOC2_TRICV Reviewed; 1481 AA.
AC P49468;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324};
OS Trieres chinensis (Marine centric diatom) (Odontella sinensis).
OG Plastid; Chloroplast.
OC Eukaryota; Sar; Stramenopiles; Ochrophyta; Bacillariophyta; Mediophyceae;
OC Biddulphiophycidae; Eupodiscales; Parodontellaceae; Trieres.
OX NCBI_TaxID=1514140;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Kowallik K.V., Stoebe B., Schaffran I., Kroth-Pancic P., Freier U.;
RT "The chloroplast genome of a chlorophyll a+c-containing alga, Odontella
RT sinensis.";
RL Plant Mol. Biol. Rep. 13:336-342(1995).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC Rule:MF_01324}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR EMBL; Z67753; CAA91746.1; -; Genomic_DNA.
DR PIR; S78373; S78373.
DR AlphaFoldDB; P49468; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 4.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 1.
DR TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Metal-binding;
KW Nucleotidyltransferase; Plastid; Transcription; Transferase; Zinc.
FT CHAIN 1..1481
FT /note="DNA-directed RNA polymerase subunit beta''"
FT /id="PRO_0000067934"
FT BINDING 217
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 291
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 298
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 301
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ SEQUENCE 1481 AA; 170975 MW; 184936FB2A577228 CRC64;
MKTYTYQNTL ISKKQLKQLL SWSFTTYDSM QACSLADELK YLGFKYASKA GISISIEDLK
VPYNKNLLLK IAHQEINSSE KIYLKGKLTD VERFQKLIDT WNLTSESLKD ELVSYFKRYD
PLNSVYIMAF SGARGNLSQV RQLVGMRGLM SDPSGELLKL PIKKNFREGL TITDYLMSGY
GARKGIIDTA LKTANSGYLT RRLIDVAQDI IIREKDCLTK HSCLIINFES NLRIKKSVYE
KILGRLLNKS IYDEKTKELI AEVNTQVTPN LIRTLKQKQI KHFYVRSPLT CSLYRSICQK
CYGWDLANEN LIDIGERIGI IAGQSIGEPG TQLTMRTFHT GGIFTSEIRG TIRSPISGIV
KFSKRLKRIP IRTNRGEDVL LTKNAGSLII IPEEKNSKPV KLELFRNTVV FHKNNQYIQK
NAIIAELVDD EKQTRTEIKP VLTTTSGEVF IPRIINKLDN INKTKLLWIL SGNVYQAPQH
SFLNFYNDYK INKNSYIFRT KLINDFSGFT VFTNSKYNLF QRILQLVSNS CWILPNSKIQ
KLQSSLNKKP YFLNIKKLKY LIDLKLLNSK YFIEISSNKH FGTLVTNNYQ TLTGGIGYYD
FRCRDRIISD NKTISYFLPW EPKKKSISLE FLTLLESQFL KSFDILSLIL KKNPDLEDCR
LAFFPNNFNL HSKVETFSHY SKQTNSLNLA WFSIVKQFPY RSVIWMSEEN YELNCDKNLL
LVEHGNFISK NFEIVPGIVS KTGGIVMISD KNKLTQEITI KTGSVYEGSL FNYFKNKIFY
PGEIILDSLK ITQPSFCECF EGRFNDQLLI RPLQVYEVPQ FKSLIQIFGT KFQTDLPFNL
TNNISYRCKS NKIIKESRNF TIIDNILDVN IRTFAKKQFE IELFSDVKKN HLNFLVSEKL
SLNHFILPQL RYTDIQSCLL VQTHQFIDSY TILGYLEVMI SKSLEIVQFK SKYKDSKQIC
LISNEDCRTI PKNSVKNKTI DNLLINNANV NYTGKILMDN DEFVTIQKGR PYFFPNCKNE
EAIINTDLIY KPLQSSSFLD NSKLKTNRLV YLNYFDITKG LINQRIHSQD IICKFSKMFI
KKNGKLYSSL LAGLINRIAI INKQLDSQQI PSCPTSIRKY REENSSKKLK KRMKRKYKKI
TGIKTLLFIK NSELNLNPLK DTQDRKNSLT VATFMLSKFY KFTGGIHSIT EDYFDEEVNS
VFCKNGEFVK NGQTIGLLNF EKEITGDIVQ GLPRVEQLLE ARKKKQITKN LPINKKKGLL
TQTTSIDSYF EFKKLGTSIK ENEKINPHNL LKVYFNYYGL IKTFFCENSY TKDSYRLFNN
YEASYRSFKK VQSLILNSVQ SVYKSQGVSI ADKHLEVIIK QMTTKVLITH QGNTSLLPRE
VIDLYHIEYI NKIARIHKKH PALYVPLLLG ITKAALNNPS FISAASFQET TRVLTKAAIE
GRVDWLRGLK ENIIIGHLMP AGTGSPVYTN CFKKSFENNL N
