RPOC2_WELMI
ID RPOC2_WELMI Reviewed; 1297 AA.
AC B2Y1V7; B7ZI51;
DT 04-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-2008, sequence version 1.
DT 03-AUG-2022, entry version 49.
DE RecName: Full=DNA-directed RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE EC=2.7.7.6 {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=PEP {ECO:0000255|HAMAP-Rule:MF_01324};
DE AltName: Full=Plastid-encoded RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
DE Short=RNA polymerase subunit beta'' {ECO:0000255|HAMAP-Rule:MF_01324};
GN Name=rpoC2 {ECO:0000255|HAMAP-Rule:MF_01324};
OS Welwitschia mirabilis (Tree tumbo) (Welwitschia bainesii).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Gnetopsida; Gnetidae; Welwitschiales; Welwitschiaceae;
OC Welwitschia.
OX NCBI_TaxID=3377;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=18452621; DOI=10.1186/1471-2148-8-130;
RA McCoy S.R., Kuehl J.V., Boore J.L., Raubeson L.A.;
RT "The complete plastid genome sequence of Welwitschia mirabilis: an
RT unusually compact plastome with accelerated divergence rates.";
RL BMC Evol. Biol. 8:130-130(2008).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=19166950; DOI=10.1016/j.ympev.2008.12.026;
RA Wu C.-S., Lai Y.-T., Lin C.-P., Wang Y.-N., Chaw S.-M.;
RT "Evolution of reduced and compact chloroplast genomes (cpDNAs) in
RT gnetophytes: Selection toward a lower-cost strategy.";
RL Mol. Phylogenet. Evol. 52:115-124(2009).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01324};
CC Note=Binds 1 Zn(2+) ion per subunit. {ECO:0000255|HAMAP-Rule:MF_01324};
CC -!- SUBUNIT: In plastids the minimal PEP RNA polymerase catalytic core is
CC composed of four subunits: alpha, beta, beta', and beta''. When a
CC (nuclear-encoded) sigma factor is associated with the core the
CC holoenzyme is formed, which can initiate transcription.
CC {ECO:0000255|HAMAP-Rule:MF_01324}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255|HAMAP-
CC Rule:MF_01324}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family. RpoC2
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01324}.
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DR EMBL; EU342371; ABY26787.1; -; Genomic_DNA.
DR EMBL; AP009568; BAH11231.1; -; Genomic_DNA.
DR RefSeq; YP_001876574.1; NC_010654.1.
DR AlphaFoldDB; B2Y1V7; -.
DR SMR; B2Y1V7; -.
DR PRIDE; B2Y1V7; -.
DR GeneID; 6276210; -.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003899; F:DNA-directed 5'-3' RNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006351; P:transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.274.100; -; 1.
DR HAMAP; MF_01324; RNApol_bact_RpoC2; 1.
DR InterPro; IPR012756; DNA-dir_RpoC2_beta_pp.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR PANTHER; PTHR19376; PTHR19376; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 1.
DR TIGRFAMs; TIGR02388; rpoC2_cyan; 1.
PE 3: Inferred from homology;
KW Chloroplast; DNA-directed RNA polymerase; Metal-binding;
KW Nucleotidyltransferase; Plastid; Transcription; Transferase; Zinc.
FT CHAIN 1..1297
FT /note="DNA-directed RNA polymerase subunit beta''"
FT /id="PRO_0000353586"
FT REGION 1278..1297
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 220
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 293
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 300
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
FT BINDING 303
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01324"
SQ SEQUENCE 1297 AA; 149498 MW; 5C28BAA0F205CD0F CRC64;
MKLKKQIRFY NKAMSRTALN ELIRRLIDRF GMMCTSHILD QLKTLGFHQA TDASFSLGID
DLLAAPSKWW LVKEEDQQGF FSEKQNLSGN LHTVETLRQL MERWHTTSEY LKEEMYPKFQ
LTNSFNPVYM MSFSGARGNK SQVHQLLGIR GLMSDPQGKI VDLPIQGNFQ EGLSLTEYII
SSYGARKGVV DTAVRTADAG YLTRRLVEVV QHIIVRKTDC GTTQGIFISP IQDRDRSKEN
VFLQKITGRV LADDVYINRR CIATRNQDIS AGLANQFKNL LIQAISIRTP FTCKSLSWIC
QLCYGRSLSH NNLIELGEAV GIIAGQSIGE PGTQLTLRTF HTGGVFTGNI VGTIRAPFNG
RIQFNSNLVY STRTFFGHPA FICRKKLFII IDDGGDKVDY SLLPTKSWVF VQNHQYVESE
QLIGEFRTTT SLLKEKVLKY IYSELNGEMH WSTFVCHALK HTQGNAHFTF GAVHLWILSG
GIYNPKVVLS FPFPKDQDQV TIAFLSTKQK NCFDPSVNLL QSNSVSFQNP KLEEEKLEEE
KLEEEPDQSS ISVPIFLENF NIQGKKQINR VLVPFFYAPK RKTKRKKRKN YPNCILKIPR
SGFLHRNTTF CIWNDSQYKS PSPGFLEYSD SPEKFVLIPE EVHFFEKSSP IAIQNKSIIR
ADTQITAKKK SQVGGLVQIH KKKTKLVVKI LPGYIDFYRK KNKKYWYKTF VSPRQNLGED
FLSEKNYFQT LNKPHRKKSF VLLRTAVEYK IPNPKEIKVP FCPDLLREED NLHIQMSNCF
YGDGGKKLQK GIQLIQTCLI FNWEQIDSTE SETSISITSI RIKNIVINTI QFSLRKHSDS
AWSQKKNQIS SKRTFPPVLD KTQSFSLSGK IQLPSNYTAT FRSLNNEKNM FLILSTSDCF
RIHLFQTKKN DNIQNKSNPN KPINNHFVGF FGHLHSIENL YPSSHFLNYN KILFNKFCYN
FNIFEIPNCY ILDEFQKVLI YPRINHLWNP KKMRYRRYSK NRYPTMNLGQ LLWENFAICK
NESFSESGQI IAVREESLVV RLAKPYLATP KATIHGNFAE IINQGDPLIT FFYERFKSSD
ITQGLPKVEQ LAEAQSNNPV VQNIEENFRI WNQDMTRTFG SFWGLFISTK ITMEQGRIHF
VDQIQKVYQS QGVHICEKHI ELIIRQMTSR VLVSDDVIFN VFLPGELIGL SRAQRIDRAF
DEAIHYKTKL LGITKASFDT PSFISEASFQ ETARVLAKAA IQGRIDWLKG LKENVILSNI
IPAGTGKKTN YSLFARRRRR KQNTKTRKNN LFSLNEK
